Podcast
Questions and Answers
What is the role of RAG-1 and RAG-2 in V(D)J recombination?
What is the role of RAG-1 and RAG-2 in V(D)J recombination?
- Creating signal joint and coding joint at the DNA level
- Recognizing and cutting DNA at the immunoglobulin-encoding region and the RSS (correct)
- Adding N-nucleotides to the cleaved strands
- Removing nonmatching nucleotides and filling in gaps
What enzyme primarily adds up to 20 N-nucleotides (non-template encoded) to the cleaved strands during V(D)J recombination?
What enzyme primarily adds up to 20 N-nucleotides (non-template encoded) to the cleaved strands during V(D)J recombination?
- Exonuclease
- Artemis
- RAG-1
- Terminal Deoxynucleotidyl Transferase (TdT) (correct)
Which process leads to diversity in the length of Complementarity Determining Regions (CDRs)?
Which process leads to diversity in the length of Complementarity Determining Regions (CDRs)?
- Repair enzymes trimming off nonmatching nucleotides
- Addition of palindromic nucleotides at overhangs
- Terminal Deoxynucleotidyl Transferase (TdT) activity (correct)
- Exonuclease removing nucleotides
What is the result of a deficiency in Terminal Deoxynucleotidyl Transferase (TdT) during V(D)J recombination?
What is the result of a deficiency in Terminal Deoxynucleotidyl Transferase (TdT) during V(D)J recombination?
Which enzyme is responsible for opening DNA hairpins during V(D)J recombination?
Which enzyme is responsible for opening DNA hairpins during V(D)J recombination?
What is the function of Covalently closed DNA hairpin ends?
What is the function of Covalently closed DNA hairpin ends?
What is notable about the Junctional Diversity process during V(D)J recombination?
What is notable about the Junctional Diversity process during V(D)J recombination?
Which proteins are crucial for creating signal joint and coding joint during V(D)J recombination?
Which proteins are crucial for creating signal joint and coding joint during V(D)J recombination?
'Many copies of V, D, and J are encoded in the germline' - This statement refers to which type of diversity in V(D)J recombination?
'Many copies of V, D, and J are encoded in the germline' - This statement refers to which type of diversity in V(D)J recombination?
What is the process by which segments are rearranged in every single B cell to create different possible antibodies?
What is the process by which segments are rearranged in every single B cell to create different possible antibodies?
In B cells, which region of the antibody defines the specificity of the binding sites?
In B cells, which region of the antibody defines the specificity of the binding sites?
What is the function of recombinase enzymes like RAG1/2 in B cell development?
What is the function of recombinase enzymes like RAG1/2 in B cell development?
Which region of the Heavy chain gene segments is exclusive to heavy chains only?
Which region of the Heavy chain gene segments is exclusive to heavy chains only?
What dictates the pairing of different segments together during gene rearrangement of BCRs?
What dictates the pairing of different segments together during gene rearrangement of BCRs?
Which rule states that a 12-bp RSS must pair with a 23-bp RSS for recombination to occur?
Which rule states that a 12-bp RSS must pair with a 23-bp RSS for recombination to occur?
In the light chain gene rearrangement, what is unique about the constant regions?
In the light chain gene rearrangement, what is unique about the constant regions?
How many different Ig isotypes are associated with the light chains of B cell receptors?
How many different Ig isotypes are associated with the light chains of B cell receptors?
