Podcast
Questions and Answers
Which temperature should the autoclave be at before opening?
Which temperature should the autoclave be at before opening?
- 100°C
- 0°C
- 65°C (correct)
- 121°C
What should be done after using the autoclave?
What should be done after using the autoclave?
- Empty the bucket collecting steam water (correct)
- Leave it on overnight
- Keep the lid open
- Fill it with tap water
What should be done to ensure the autoclave reaches the right pressure?
What should be done to ensure the autoclave reaches the right pressure?
- Keep the lid open
- Ensure the air vent is closed
- Move the lid counterclockwise (correct)
- Fill it with tap water
What does the pressure gauge popping out indicate?
What does the pressure gauge popping out indicate?
When can the air vent be opened after the sterilization process?
When can the air vent be opened after the sterilization process?
What temperature should the duran bottle reach before it can be opened?
What temperature should the duran bottle reach before it can be opened?
How often should the consumables of the Sartorius Arium Mini Water System be changed?
How often should the consumables of the Sartorius Arium Mini Water System be changed?
Which program has a stay warm option?
Which program has a stay warm option?
What is the temperature for Program 20?
What is the temperature for Program 20?
How long does Program 2 run for?
How long does Program 2 run for?
What should be the volume of water in the duran when autoclaving 3x 1L agar bottles?
What should be the volume of water in the duran when autoclaving 3x 1L agar bottles?
How often should the temperature probe be cleaned?
How often should the temperature probe be cleaned?
When should the start time be set if the run needs to finish at 9AM?
When should the start time be set if the run needs to finish at 9AM?
What should be done with the probe when the autoclave is not being used?
What should be done with the probe when the autoclave is not being used?
Which agar needs to be supplemented to make it selective?
Which agar needs to be supplemented to make it selective?
What is the purpose of gently swirling the agar after autoclaving?
What is the purpose of gently swirling the agar after autoclaving?
How can bubbles form in the agar?
How can bubbles form in the agar?
What is the temperature used to heat the media when sterilizing by boiling?
What is the temperature used to heat the media when sterilizing by boiling?
What is the purpose of filtering the media?
What is the purpose of filtering the media?
What is the recommended filter size for filtering the media?
What is the recommended filter size for filtering the media?
What should be done with the broths after they are prepared?
What should be done with the broths after they are prepared?
According to the text, what is the recommended temperature for pouring agar into petri dishes?
According to the text, what is the recommended temperature for pouring agar into petri dishes?
What is the suggested amount of agar to be poured into a petri dish?
What is the suggested amount of agar to be poured into a petri dish?
How many plates can be made from 1L of agar if pouring 20ml per petri dish?
How many plates can be made from 1L of agar if pouring 20ml per petri dish?
What is the recommended drying time for plates in the biosafety cabinet after setting?
What is the recommended drying time for plates in the biosafety cabinet after setting?
What is the consequence of placing wet plates in the fridge before use?
What is the consequence of placing wet plates in the fridge before use?
What is the recommended temperature for pouring agar on live bacterial samples?
What is the recommended temperature for pouring agar on live bacterial samples?
Where should plates be placed for incubation lasting 3+ days?
Where should plates be placed for incubation lasting 3+ days?
How long can plates be stored in a cool dry place?
How long can plates be stored in a cool dry place?
Where should media with selective agents be stored?
Where should media with selective agents be stored?
Which container should be filled with water if not washed immediately to prevent the agar from setting?
Which container should be filled with water if not washed immediately to prevent the agar from setting?
What should be added to the contaminated or more than a month old media in durans before autoclaving?
What should be added to the contaminated or more than a month old media in durans before autoclaving?
What needs to be removed from the duran bottle before washing all the components thoroughly?
What needs to be removed from the duran bottle before washing all the components thoroughly?
