Autoclaving and Media Preparation

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Which temperature should the autoclave be at before opening?

100°C

0°C

65°C (correct)

121°C

What should be done after using the autoclave?

Empty the bucket collecting steam water (correct)

Leave it on overnight

Keep the lid open

Fill it with tap water

What should be done to ensure the autoclave reaches the right pressure?

Keep the lid open

Ensure the air vent is closed

Move the lid counterclockwise (correct)

Fill it with tap water

What does the pressure gauge popping out indicate?

The autoclave has reached the right pressure Signup and view all the answers

When can the air vent be opened after the sterilization process?

After the tick button lights up Signup and view all the answers

What temperature should the duran bottle reach before it can be opened?

60°C Signup and view all the answers

How often should the consumables of the Sartorius Arium Mini Water System be changed?

Every 6 months Signup and view all the answers

Which program has a stay warm option?

Program 1 Signup and view all the answers

What is the temperature for Program 20?

131°C Signup and view all the answers

How long does Program 2 run for?

15 minutes Signup and view all the answers

What should be the volume of water in the duran when autoclaving 3x 1L agar bottles?

1L Signup and view all the answers

How often should the temperature probe be cleaned?

Once a week Signup and view all the answers

When should the start time be set if the run needs to finish at 9AM?

6AM Signup and view all the answers

What should be done with the probe when the autoclave is not being used?

Take it out of water Signup and view all the answers

Which agar needs to be supplemented to make it selective?

Agar that is sold as base Signup and view all the answers

What is the purpose of gently swirling the agar after autoclaving?

To prevent settling at the bottom Signup and view all the answers

How can bubbles form in the agar?

By shaking the hot agar Signup and view all the answers

What is the temperature used to heat the media when sterilizing by boiling?

100°C Signup and view all the answers

What is the purpose of filtering the media?

To remove particulates Signup and view all the answers

What is the recommended filter size for filtering the media?

0.2 µm Signup and view all the answers

What should be done with the broths after they are prepared?

Store them in allocated boxes on the shelving Signup and view all the answers

According to the text, what is the recommended temperature for pouring agar into petri dishes?

60°C Signup and view all the answers

What is the suggested amount of agar to be poured into a petri dish?

20ml Signup and view all the answers

How many plates can be made from 1L of agar if pouring 20ml per petri dish?

40 plates Signup and view all the answers

What is the recommended drying time for plates in the biosafety cabinet after setting?

30-45 mins Signup and view all the answers

What is the consequence of placing wet plates in the fridge before use?

All of the above Signup and view all the answers

What is the recommended temperature for pouring agar on live bacterial samples?

45°C Signup and view all the answers

Where should plates be placed for incubation lasting 3+ days?

At the bottom and left-hand side of the incubator Signup and view all the answers

How long can plates be stored in a cool dry place?

Up to a month Signup and view all the answers

Where should media with selective agents be stored?

In a cool dark place Signup and view all the answers

Which container should be filled with water if not washed immediately to prevent the agar from setting?

Duran Signup and view all the answers

What should be added to the contaminated or more than a month old media in durans before autoclaving?

Autoclave tape with "X" Signup and view all the answers

What needs to be removed from the duran bottle before washing all the components thoroughly?

Drip ring Signup and view all the answers