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Questions and Answers
How is light intensity controlled? ______ of the lamp from the set up.
Adjust the distance
What is the function of the muslin cloth? To ______ out any debris in the ground leaf mixture but allowing chloroplasts to pass through.
filter
Why are the stalks of leaves removed before grinding? The stalks do not contain many ______.
chloroplasts
Outline the procedure of investigating the effect of light intensity, after chloroplast extract has been obtained. 1. 2. 3. 4. Set the ______ to the red filter. Zero using a cuvette containing chloroplast extract and distilled water. Place test tube in the rack 30cm from light source and add DCPIP.
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How is light intensity controlled? Perform the practical in a ______ so that the only light source is the lamp.
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Why are the stalks of leaves removed before grinding? The stalks do not contain many ______.
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What is the function of dehydrogenase in chloroplasts? It catalyses the acceptance of electrons by NADP in the light dependent ______
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What is the function of DCPIP in the experimental setup?
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Why is the plant extract chilled in an ice-water bath before the experiment?
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What is the role of dehydrogenase in chloroplasts during the light-dependent reactions?
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How does DCPIP indicate the reduction of electrons in the experiment?
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Explain the setup of the control in the experiment.
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What is the purpose of performing the practical in a dark room with the lamp as the only light source?
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Why is it important to adjust the distance of the lamp from the set up during the experiment?
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What role does the muslin cloth play in the experiment involving the ground leaf mixture?
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Why are the stalks of leaves removed before grinding?
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After obtaining the chloroplast extract, what steps are involved in investigating the effect of light intensity?
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Study Notes
Dehydrogenase Activity in Chloroplasts
- Dehydrogenase catalyses the acceptance of electrons by NADP in the light dependent reactions.
DCPIP
- DCPIP is a redox indicator dye that acts as an alternate electron acceptor instead of NADP.
- It turns from blue to colourless when reduced.
Preparing Plant Extract
- Remove stalks from leaves before grinding because they do not contain many chloroplasts.
- Use muslin cloth to filter out debris from the ground leaf mixture, allowing chloroplasts to pass through.
- Chill the plant extract in an ice-water bath to lower the activity of enzymes and prevent them from breaking down the chloroplasts.
Investigating Light Intensity
- To control light intensity, adjust the distance of the lamp from the setup and perform the practical in a dark room.
- Set up a control by filling a cuvette with chloroplast extract and distilled water.
- Procedure to investigate the effect of light intensity:
- Set the colorimeter to the red filter.
- Zero using a cuvette containing chloroplast extract and distilled water.
- Place the test tube in the rack 30cm from the light source and add DCPIP.
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Description
Test your knowledge on the function of dehydrogenase in chloroplasts with this flashcard set from PMT Education. Learn about its role in catalyzing the acceptance of electrons by NADP in the light-dependent process.
