Podcast
Play an AI-generated podcast conversation about this lesson
Questions and Answers
What is the method to synthesize antisense cDNA or RNA?
What is the method to synthesize antisense cDNA or RNA?
- Reverse transcription with a primer corresponding to the antisense strand of the target mRNA (correct)
- Polymerase chain reaction with a primer corresponding to the sense strand of the target mRNA
- Transcription using a primer complementary to the sense strand of the target mRNA
- Ligation of random DNA fragments to form the antisense sequence
Why is a phosphodiester bond preferred for joining nucleotides in DNA oligonucleotides?
Why is a phosphodiester bond preferred for joining nucleotides in DNA oligonucleotides?
- It enhances the flexibility of the DNA strand
- It allows for easier separation of the nucleotides during replication
- It provides greater structural stability and resistance to nucleolytic degradation (correct)
- It increases the rate of transcription of the DNA strand
What are interfering RNAs (iRNAs) primarily composed of?
What are interfering RNAs (iRNAs) primarily composed of?
- DNA molecules with specific gene sequences
- Long non-coding RNA molecules
- mRNA molecules with specific gene sequences
- Small RNA molecules, including siRNA and miRNA (correct)
Which method is a nucleic acid delivery method?
Which method is a nucleic acid delivery method?
Signup and view all the answers
What is an example of using antisense oligonucleotides to study gene function?
What is an example of using antisense oligonucleotides to study gene function?
Signup and view all the answers
Flashcards are hidden until you start studying
