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Questions and Answers
What is the method to synthesize antisense cDNA or RNA?
What is the method to synthesize antisense cDNA or RNA?
- Reverse transcription with a primer corresponding to the antisense strand of the target mRNA (correct)
- Polymerase chain reaction with a primer corresponding to the sense strand of the target mRNA
- Transcription using a primer complementary to the sense strand of the target mRNA
- Ligation of random DNA fragments to form the antisense sequence
Why is a phosphodiester bond preferred for joining nucleotides in DNA oligonucleotides?
Why is a phosphodiester bond preferred for joining nucleotides in DNA oligonucleotides?
- It enhances the flexibility of the DNA strand
- It allows for easier separation of the nucleotides during replication
- It provides greater structural stability and resistance to nucleolytic degradation (correct)
- It increases the rate of transcription of the DNA strand
What are interfering RNAs (iRNAs) primarily composed of?
What are interfering RNAs (iRNAs) primarily composed of?
- DNA molecules with specific gene sequences
- Long non-coding RNA molecules
- mRNA molecules with specific gene sequences
- Small RNA molecules, including siRNA and miRNA (correct)
Which method is a nucleic acid delivery method?
Which method is a nucleic acid delivery method?
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What is an example of using antisense oligonucleotides to study gene function?
What is an example of using antisense oligonucleotides to study gene function?
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