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Antimicrobial Resistance Testing

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Questions and Answers

What gene is responsible for the production of altered PBP2a in MRSA?

erm gene

mecA gene (correct)

van A gene

bla gene

Which test is specifically used for VRSA identification?

Etest (correct)

Cefoxitin Disk test

Modified Hodge Test

B-lactamase test

Which of the following tests is used for detecting Extended Spectrum Beta-Lactamases (ESBL)?

Etest

Cefoxitin Disk test

Test with and without clavulanic acid (correct)

Van A PCR

What is the purpose of the Modified Hodge Test?

To test for carbapenemase production Signup and view all the answers

What does the Cefoxitin Disk test primarily assess?

Methicillin resistance in Staphylococcus species Signup and view all the answers

Which test is used to detect the presence of the Erm gene associated with Clindamycin resistance?

van A PCR Signup and view all the answers

For testing ESBL-producing organisms, which combination of substances is important?

Clavulanic acid and cefotaxime Signup and view all the answers

What is the primary use of the Etest in antimicrobial resistance testing?

To determine minimum inhibitory concentration (MIC) Signup and view all the answers

Which method is employed to identify the presence of carbapenemase?

Modified Hodge Test Signup and view all the answers

Which gene is associated with methicillin resistance in Staphylococcus aureus?

mecA gene Signup and view all the answers

Study Notes

Antimicrobial Resistance Testing

Methicillin-resistant Staphylococcus aureus (MRSA): The mecA gene alters penicillin-binding protein 2a (PBP2a).
Vancomycin-resistant Staphylococcus aureus (VRSA): Testing involves the Etest method.
Extended-spectrum beta-lactamases (ESBL): Testing includes both a standard test and one with clavulanic acid.
Clindamycin resistance: The erm gene is associated with this resistance.
Carbapenemase production: Detection relies on the Modified Hodge Test.
Glycopeptide resistance: The vanA gene is tested for using PCR.
Oxacillin resistance: The cefoxitin disk diffusion test can help identify this.
Penicillin resistance: Often due to beta-lactamase production.

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Description

Test your knowledge on various methods of antimicrobial resistance testing. This quiz covers key pathogens like MRSA and VRSA, along with specific resistance mechanisms and laboratory techniques. Perfect for students and professionals in microbiology and infectious diseases!