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Antimicrobial Agent Use Procedure Quiz

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Questions and Answers

What is the purpose of rotating the Mueller-Hinton plate after each streaking?

To enhance the growth of all bacteria on the plate

To create a uniform layer of culture across the surface (correct)

To ensure complete drying of the agar surface

To facilitate equal distribution of antibiotics later

Why should the antibiotic disk not be pressed into the agar during the dispensing process?

It enhances the effectiveness of the antibiotics

It may lead to inaccurate measurement of inhibition zones (correct)

It could cause the antibiotic to be absorbed improperly

It can increase the spread of bacteria on the plate

What is the recommended temperature and time for incubating the agar plates?

40°C for 10 hours

35°C for 16-18 hours (correct)

25°C for 12 hours

30°C for 24 hours

What should be done before removing excess culture from the cotton swab?

Squeeze the swab against the inner side of the tube (A) Signup and view all the answers

How should the zones of inhibition be measured after the incubation period?

To the nearest mm for each antibiotic (C) Signup and view all the answers

What is the first step in the procedure prior to inoculating the agar plates?

Mark the lid with the date and the bacterium's name (D) Signup and view all the answers

What is the significance of allowing the culture to dry for 5-10 minutes at room temperature?

To prevent dilution of bacteria during antibiotic application (A) Signup and view all the answers

During the antibacterial sensitivity analysis, what does a zone of inhibition indicate?

Susceptibility of bacteria to the antibiotic (D) Signup and view all the answers

Study Notes

Antimicrobial Agent Use Procedure

Mark each Mueller-Hinton agar plate with date and bacterium name.
Use a separate sterile swab for each bacterium.
Immerse swab in culture tube, squeeze excess culture.
Streak swab across plate surface three times, rotating 60 degrees after each.
Streak swab around agar edge to ensure complete seeding.
Allow culture to dry for 5-10 minutes at room temperature.
Dispense antibiotics, avoiding contact with culture.
Use alcohol-flamed forceps to gently press disks onto agar.
Do not press disks into agar or move them after placement.
Incubate plates for 16-18 hours at 35°C.
Do not invert plates during incubation.
Measure zones of inhibition to nearest mm.
Determine if bacteria are resistant or susceptible to each antibiotic.

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Description

Test your knowledge on the procedure for using antimicrobial agents in laboratory settings. This quiz will cover the essential steps and techniques for properly conducting an antimicrobial susceptibility test using Mueller-Hinton agar plates. Assess your understanding of the processes involved from plating to measuring inhibition zones.