Antimicrobial Agent Use Procedure Quiz

A specific type of agar used for testing antimicrobial sensitivity, providing a standardized environment for bacterial growth. It is formulated with beef extract, casein hydrolysate, and starch, creating a nutrient-rich media that allows for the growth of many bacterial species.

The process of applying a uniform layer of bacteria onto the Mueller-Hinton agar surface. The swabbing should be done in a controlled manner to ensure even distribution of the bacteria for accurate results.

The clear area surrounding the antibiotic disk where bacteria cannot grow. The size of the zone indicates the effectiveness of the specific antibiotic against the bacteria.

The susceptibility of bacteria to a specific antibiotic is determined by measuring the zone of inhibition and comparing it to predetermined standards. The result will indicate if the bacterium is susceptible, intermediate, or resistant to the antibiotic.

The application of antibiotic disks onto the inoculated agar plate, ensuring the disks are properly placed at equal distances for uniform results.

The period of time the plates with antibiotic disks are incubated at a specific temperature to allow bacterial growth and the formation of zones of inhibition.

The precise measurement of the zone of inhibition for each antibiotic disk, using millimeters as the unit of measurement. This data is crucial for determining the susceptibility of bacteria to specific antibiotics.

The classification of bacteria based on their sensitivity to the antibiotic. Bacteria can be classified as "susceptible", "intermediate", or "resistant" depending on the size of the zone of inhibition.