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Questions and Answers
What is the purpose of removing the connections between the amino acid side chains of the antigen and the formol?
What is the purpose of removing the connections between the amino acid side chains of the antigen and the formol?
- To enhance the staining process
- To increase the binding capacity of the antigen
- To reveal the antigenic regions in IHC application (correct)
- To reduce the temperature of the solution
What is the optimal temperature range for applying the solutions in the physical method of antigen unmasking?
What is the optimal temperature range for applying the solutions in the physical method of antigen unmasking?
- 100-105°C
- 90-95°C
- 95-100°C (correct)
- 80-90°C
Which of the following enzymes is NOT used in the chemical method of antigen unmasking?
Which of the following enzymes is NOT used in the chemical method of antigen unmasking?
- Pepsin
- Trypsin
- Proteinase K
- Lipase (correct)
What is the most widely used chromogen in histochemistry?
What is the most widely used chromogen in histochemistry?
What is the primary aim of antigen unmasking in IHC?
What is the primary aim of antigen unmasking in IHC?
What is the purpose of incubating the sample at 37°C for 10-20 minutes in a humid box during the chemical method of antigen unmasking?
What is the purpose of incubating the sample at 37°C for 10-20 minutes in a humid box during the chemical method of antigen unmasking?
What is the purpose of incubating the primary antibody for 1 hour?
What is the purpose of incubating the primary antibody for 1 hour?
What is the role of DAB in immunohistochemistry?
What is the role of DAB in immunohistochemistry?
Why are sections treated with hydrogen peroxide?
Why are sections treated with hydrogen peroxide?
What is the purpose of using PBS solution in immunohistochemistry?
What is the purpose of using PBS solution in immunohistochemistry?
What is the purpose of dehydrating sections through graded alcohols?
What is the purpose of dehydrating sections through graded alcohols?
Why are slides coated with Poly-L-lysine solution?
Why are slides coated with Poly-L-lysine solution?
What is a major disadvantage of using avidin in the avidin-biotin method?
What is a major disadvantage of using avidin in the avidin-biotin method?
What is the primary difference between avidin and streptavidin in the avidin-biotin method?
What is the primary difference between avidin and streptavidin in the avidin-biotin method?
What is the labeled antigen method used for in immunohistochemistry?
What is the labeled antigen method used for in immunohistochemistry?
What is the purpose of the avidin-biotin-enzyme complex in the avidin-biotin method?
What is the purpose of the avidin-biotin-enzyme complex in the avidin-biotin method?
What is the peroxidase-antiperoxidase (PAP) method used for in immunohistochemistry?
What is the peroxidase-antiperoxidase (PAP) method used for in immunohistochemistry?
What is the most widely used chromogen in histochemistry?
What is the most widely used chromogen in histochemistry?
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