Podcast
Questions and Answers
What is the potential issue with using an isotype control antibody in an experiment?
What is the potential issue with using an isotype control antibody in an experiment?
The nonspecific binding can lead to false-positive results.
How can you prevent unspecific binding of the antibody Fc-region to Fc-receptor?
How can you prevent unspecific binding of the antibody Fc-region to Fc-receptor?
You can block the FC-receptors with a blocking agent like bovine serum albumin or milk.
Why is Alexa-fluor 633 chosen as the optimal election for envoplakin staining?
Why is Alexa-fluor 633 chosen as the optimal election for envoplakin staining?
Because it is a better option than Alexa-fluor 555 for visualization of envoplakin.
What is the potential consequence of nonspecific binding in immunostaining and fluorescence microscopy?
What is the potential consequence of nonspecific binding in immunostaining and fluorescence microscopy?
What can the available microscope contain to ensure proper visualization of the indicated dyes?
What can the available microscope contain to ensure proper visualization of the indicated dyes?
What is the emission peak of DAPI dye?
What is the emission peak of DAPI dye?
What is the emission peak of Propidium Iodide (PI)?
What is the emission peak of Propidium Iodide (PI)?
What is the emission peak of the secondary antibody used for actin visualization?
What is the emission peak of the secondary antibody used for actin visualization?
What is the emission peak of the secondary antibody used for envoplakin visualization?
What is the emission peak of the secondary antibody used for envoplakin visualization?
Why is it essential to choose a counterstain with minimal spectral overlap with the fluorophores?
Why is it essential to choose a counterstain with minimal spectral overlap with the fluorophores?
Why is DAPI considered a suitable choice for counterstaining the cell nuclei?
Why is DAPI considered a suitable choice for counterstaining the cell nuclei?
What does spectral overlap with fluorophores cause?
What does spectral overlap with fluorophores cause?
How does DAPI's emission peak minimize interference with other fluorophores?
How does DAPI's emission peak minimize interference with other fluorophores?
What is the purpose of using a counterstain in this context?
What is the purpose of using a counterstain in this context?
How does DAPI's emission peak align with the emission peaks of the secondary antibodies used?
How does DAPI's emission peak align with the emission peaks of the secondary antibodies used?
What is the purpose of creating gates on negative and positive populations in flow cytometric analysis?
What is the purpose of creating gates on negative and positive populations in flow cytometric analysis?
How is the stain index calculated in flow cytometric analysis?
How is the stain index calculated in flow cytometric analysis?
What is the purpose of creating a forward scatter/side scatter gate in flow cytometric analysis?
What is the purpose of creating a forward scatter/side scatter gate in flow cytometric analysis?
What is the significance of determining the optimal concentration of the CD25 antibody in flow cytometric analysis?
What is the significance of determining the optimal concentration of the CD25 antibody in flow cytometric analysis?
What is the purpose of titrating the CD25 antibody at different concentrations?
What is the purpose of titrating the CD25 antibody at different concentrations?
What is the function of the CD25 antibody in flow cytometric analysis?
What is the function of the CD25 antibody in flow cytometric analysis?
What is the purpose of creating fluorescence histograms gated on forward/side scatter in flow cytometric analysis?
What is the purpose of creating fluorescence histograms gated on forward/side scatter in flow cytometric analysis?
What surface markers need to be detected on adipose-derived stem cells (ASCs)?
What surface markers need to be detected on adipose-derived stem cells (ASCs)?
What is the expression of CD73, CD90, CD105, and CD31 on ASCs?
What is the expression of CD73, CD90, CD105, and CD31 on ASCs?
What is the setting of the flow cytometer used in the lab?
What is the setting of the flow cytometer used in the lab?
Which antibody and detector should be selected for each surface antigen? Provide an explanation.
Which antibody and detector should be selected for each surface antigen? Provide an explanation.
What is the spillover source to be analyzed?
What is the spillover source to be analyzed?
What is the fixable viability dye used in the 'Viability' folder?
What is the fixable viability dye used in the 'Viability' folder?
How many samples are there in the 'Viability' folder?
How many samples are there in the 'Viability' folder?
What is the surface marker stained in the 'dilution' folder?
What is the surface marker stained in the 'dilution' folder?
Where can the information about the optimal concentration to stain the lymphocytes be found?
Where can the information about the optimal concentration to stain the lymphocytes be found?
