Anthrax and BSL-3 Safety Procedures

Bacillus anthracis and Anthrax

• Bacillus anthracis is a Gram-positive, rod-shaped bacterium responsible for Anthrax.
• Anthrax is not highly contagious, contradicting the belief that it easily spreads between individuals.
• Classified as Hazard Group 3 due to its potential to spread to the community, but effective treatments are available.

HEPA Filters and Containment Levels

• HEPA stands for High-Efficiency Particulate Air, indicating its effectiveness in filtering airborne particles.
• Containment Level 3 (BSL-3) is the standard for handling Anthrax, while Ebola requires Containment Level 4 (BSL-4).

Safety in BSL-3 Laboratories

• Class III mode provides a protective barrier against microbial contamination.
• In Class I mode, the night panel should be removed for optimal airflow into the cabinet.
• Side panels in Class I mode should remain on to enhance airflow into the cabinet's front aperture.
• Negative air pressure in BSL-3 labs is crucial to prevent microorganisms from escaping.

Personal Protective Equipment (PPE) and Cleanliness

• Gloves must be worn at all times in BSL-3 labs; oversleeves are necessary only when working within the cabinet.
• Personal protection equipment must be donned and removed in designated areas.
• Overshoes are put on in anterooms and removed inside the lab for contamination control.

Lab Protocols and Sterility Testing

• Sterility tests are essential to confirm cultures are free from contamination before leaving the lab.
• A 5% Biocleanse solution must be used for cleaning work surfaces at the end of the working day.
• The CLEAN sign on the biological safety cabinet is displayed when no hazardous materials are present.

Lab Access and Safety Measures

• Signing in and out of the lab is mandatory to maintain access control and track authorized personnel.
• During fumigation, fridge and freezer doors should not be opened or cleaned to ensure safety protocols are followed.