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Questions and Answers
What type of pathogen is Bacillus anthracis?
Gram-positive, rod-shaped bacterium
Which of these statements about anthrax is NOT correct?
What does HEPA stand for in HEPA filter?
High-Efficiency Particulate Air
What factor applies to Class III mode?
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In Class I mode, should the night panel be removed or attached?
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Should we put on the side panel in Class I mode?
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Why is Bacillus anthracis classified as Hazard Group 3?
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Which facilities must be used if we have an Ebola sample?
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Why is it important that negative air pressure is maintained inside the BSL-3 lab?
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When should gloves and oversleeves be worn in a BSL-3 or CL3 lab?
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Which of these factors is NOT important regarding your buddy Marie after training?
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When should the CLEAN sign be used on the BSC?
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Why is it important that a sterility test is carried out?
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What should all work surfaces be cleaned with at the end of each working day?
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Which of these statements about personal protection equipment is correct?
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Why do all staff need to sign in and out of the lab?
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When fumigating the lab, which of the following should NOT be carried out?
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Study Notes
Bacillus anthracis and Anthrax
- Bacillus anthracis is a Gram-positive, rod-shaped bacterium responsible for Anthrax.
- Anthrax is not highly contagious, contradicting the belief that it easily spreads between individuals.
- Classified as Hazard Group 3 due to its potential to spread to the community, but effective treatments are available.
HEPA Filters and Containment Levels
- HEPA stands for High-Efficiency Particulate Air, indicating its effectiveness in filtering airborne particles.
- Containment Level 3 (BSL-3) is the standard for handling Anthrax, while Ebola requires Containment Level 4 (BSL-4).
Safety in BSL-3 Laboratories
- Class III mode provides a protective barrier against microbial contamination.
- In Class I mode, the night panel should be removed for optimal airflow into the cabinet.
- Side panels in Class I mode should remain on to enhance airflow into the cabinet's front aperture.
- Negative air pressure in BSL-3 labs is crucial to prevent microorganisms from escaping.
Personal Protective Equipment (PPE) and Cleanliness
- Gloves must be worn at all times in BSL-3 labs; oversleeves are necessary only when working within the cabinet.
- Personal protection equipment must be donned and removed in designated areas.
- Overshoes are put on in anterooms and removed inside the lab for contamination control.
Lab Protocols and Sterility Testing
- Sterility tests are essential to confirm cultures are free from contamination before leaving the lab.
- A 5% Biocleanse solution must be used for cleaning work surfaces at the end of the working day.
- The CLEAN sign on the biological safety cabinet is displayed when no hazardous materials are present.
Lab Access and Safety Measures
- Signing in and out of the lab is mandatory to maintain access control and track authorized personnel.
- During fumigation, fridge and freezer doors should not be opened or cleaned to ensure safety protocols are followed.
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Description
Test your knowledge on Bacillus anthracis, its role in anthrax, and the safety measures required in BSL-3 laboratories. This quiz covers effective treatments, containment levels, and laboratory safety protocols. Understand the importance of HEPA filters and airflow in preventing contamination.