Podcast
Questions and Answers
Which test directly assesses the size of red blood cells?
Which test directly assesses the size of red blood cells?
- Hematocrit (Hct)
- Hemoglobin (Hb)
- Red Blood Cell Count
- Mean Corpuscular Volume (MCV) (correct)
What does MCHC reflect?
What does MCHC reflect?
- White blood cell count
- Platelet count
- RBC count
- Hemoglobin concentration in RBCs (correct)
Which test measures the variation in RBC size?
Which test measures the variation in RBC size?
- Hematocrit (Hct)
- Hemoglobin (Hb)
- Red Cell Distribution Width (RDW) (correct)
- Mean Corpuscular Volume (MCV)
What is indicated by an increased reticulocyte count?
What is indicated by an increased reticulocyte count?
What is the purpose of a peripheral blood smear?
What is the purpose of a peripheral blood smear?
What's the role of serum ferritin in anemia diagnosis?
What's the role of serum ferritin in anemia diagnosis?
Deficiencies in which vitamin(s) lead to macrocytic anemia?
Deficiencies in which vitamin(s) lead to macrocytic anemia?
What is TIBC a measure of?
What is TIBC a measure of?
In hemoglobin electrophoresis, what causes proteins to migrate at different rates?
In hemoglobin electrophoresis, what causes proteins to migrate at different rates?
The CBC includes measurement of which of the following?
The CBC includes measurement of which of the following?
What does a low hemoglobin level typically indicate?
What does a low hemoglobin level typically indicate?
Which of the following is used in supravital staining for reticulocyte count?
Which of the following is used in supravital staining for reticulocyte count?
What term describes the variation in RBC shape?
What term describes the variation in RBC shape?
What is determined by measuring Mean Corpuscular Volume (MCV)?
What is determined by measuring Mean Corpuscular Volume (MCV)?
In hemoglobin electrophoresis, what pH is used with cellulose acetate?
In hemoglobin electrophoresis, what pH is used with cellulose acetate?
Which of the following reflects Hb concentration in RBCs?
Which of the following reflects Hb concentration in RBCs?
Anemia severity is indicated by which measurements in CBC?
Anemia severity is indicated by which measurements in CBC?
What does a reticulocyte count measure?
What does a reticulocyte count measure?
Which of these is a cause of macrocytic anemia?
Which of these is a cause of macrocytic anemia?
Serum iron along with what other test helps assess iron availability?
Serum iron along with what other test helps assess iron availability?
Flashcards
RBC count, Hemoglobin (Hb), Hematocrit (Hct)
RBC count, Hemoglobin (Hb), Hematocrit (Hct)
Indicates the severity of anemia.
Mean Corpuscular Volume (MCV)
Mean Corpuscular Volume (MCV)
Determines red blood cell size (microcytic, normocytic, or macrocytic).
Mean Corpuscular Hemoglobin Concentration (MCHC)
Mean Corpuscular Hemoglobin Concentration (MCHC)
Reflects the concentration of hemoglobin in red blood cells.
Red Cell Distribution Width (RDW)
Red Cell Distribution Width (RDW)
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Reticulocyte Count
Reticulocyte Count
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Peripheral Blood Smear
Peripheral Blood Smear
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Anisocytosis
Anisocytosis
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Poikilocytosis
Poikilocytosis
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Serum Ferritin
Serum Ferritin
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Serum Iron and TIBC
Serum Iron and TIBC
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Vitamin B12 and Folate Levels
Vitamin B12 and Folate Levels
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Hemoglobin Electrophoresis Principle
Hemoglobin Electrophoresis Principle
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Study Notes
- Routine diagnostic tests are performed to diagnose anemia in patients
Learning Objectives
- To explain the purpose and significance of routine diagnostic tests to evaluate anemia
- Diagnostic tests discussed are:
- Complete Blood Count (CBC)
- Reticulocyte Count
- Peripheral Blood Smear
- Serum Ferritin
- Serum Iron
- TIBC (Total Iron-Binding Capacity)
- Vitamin B12/Folate Levels
- Hemoglobin Electrophoresis
Complete Blood Count (CBC)
- RBC Count, Hemoglobin (Hb), and Hematocrit (Hct) indicate anemia severity
- Mean Corpuscular Volume (MCV) determines RBC size such as:
- Microcytic anemia
- Normocytic anemia
- Macrocytic anemia
- Mean Corpuscular Hemoglobin Concentration (MCHC) reflects Hb concentration in RBCs
- Red Cell Distribution Width (RDW) measures variation in RBC size, indicating anisocytosis
Reticulocyte Count
- Reticulocyte Count measures bone marrow response to anemia
- Increased in cases of hemolysis or blood loss
- Decreased in production-related anemias
- Performed using supravital staining such as:
- New Methylene Blue
- Brilliant Cresyl Green
Peripheral Blood Smear
- A peripheral blood smear examines RBC morphology - size, shape, and inclusions
- It also identifies:
- Anisocytosis
- Poikilocytosis
Serum Chemistry
- Serum Ferritin, Serum Iron, and Total Iron-Binding Capacity (TIBC) are assessed
- Ferritin indicates iron stores, while serum iron and TIBC indicate iron availability
- Vitamin B12 and Folate Levels are assessed; deficiencies lead to macrocytic anemia
Hemoglobin Electrophoresis
- Differently charged proteins migrate at different rates across the charged agarose or cellulose acetate gel in Hemoglobin Electrophoresis
- Alkaline pH: Cellulose Acetate (pH 8.4)
- Acidic pH: Agarose gel (pH 6.0)
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