Analytical Sensitivity Comparison: NanoVue vs NanoDrop
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Questions and Answers

Which method shows more variability among its measurements?

  • Method B (correct)
  • Both methods show the same variability
  • Method A
  • Cannot be determined from the given information

    • Which method suggests lower precision compared to the other?

  • Cannot be determined from the given information
  • Method A
  • Both methods have the same precision
  • Method B (correct)

    • At a 95% interval, which method is more accurate?

  • Both methods have the same accuracy
  • Cannot be determined from the given information
  • Method B
  • Method A (correct)

    • What is the purpose of the 'Albumin Tester' developed by the researchers?

    <p>To detect early occurrence of kidney disease</p> Signup and view all the answers

    What is the Lower Limit of Detection (LLOD)?

    <p>The lowest concentration of an analyte that can be reliably detected but not necessarily quantified</p> Signup and view all the answers

    What do blank measurements using a saline solution help to establish?

    <p>Variability of the urine sample measurements</p> Signup and view all the answers

    What is the purpose of performing control measurements using a saline solution?

    <p>To establish a baseline for detecting analyte concentration</p> Signup and view all the answers

    What is indicated by a larger standard deviation in Method B's measurements?

    <p><strong>Lower</strong> precision compared to Method A</p> Signup and view all the answers

    What is the purpose of the blocking step in the ELISA process?

    <p>To prevent nonspecific binding on any remaining surface of the microplate</p> Signup and view all the answers

    Which enzyme is commonly used in ELISA as an enzyme-linked antibody?

    <p>Horseradish peroxidase (HRP)</p> Signup and view all the answers

    What substance is commonly used as a blocking agent in the ELISA process?

    <p>Bovine serum albumin (BSA)</p> Signup and view all the answers

    During which step is the microplate coated with an antigen or an antibody specific to the cytokine of interest?

    <p>Coating</p> Signup and view all the answers

    What is the purpose of washing the plate in the ELISA process?

    <p>To remove unbound substances and reduce background noise</p> Signup and view all the answers

    What occurs if the cytokine is present in the samples during the ELISA process?

    <p>Formation of an immune complex with the coated antibody or antigen</p> Signup and view all the answers

    What is added to the plate as enzyme-conjugated antibodies in the ELISA process?

    <p>Horseradish peroxidase (HRP)</p> Signup and view all the answers

    What is measured using antibodies and enzymes in the ELISA process?

    <p>Concentration of a specific antigen</p> Signup and view all the answers

    What does a flatter curve in terms of analytical sensitivity indicate?

    <p>Lower analytical sensitivity</p> Signup and view all the answers

    In the context of analytical sensitivity, what does a steeper slope indicate?

    <p>Higher analytical sensitivity</p> Signup and view all the answers

    What is the purpose of comparing the performance of a new microliter-based spectrophotometer to the well-established NanoDrop?

    <p>To assess the performance relative to an established instrument</p> Signup and view all the answers

    In the given context, what does it mean when larger changes in concentration are needed to produce noticeable differences in absorbance?

    <p>Lower analytical sensitivity</p> Signup and view all the answers

    What does the steepness of curves in spectrophotometry graphs indicate?

    <p>The speed of the absorbance changes with respect to concentration changes</p> Signup and view all the answers

    What is the significance of a steeper curve on a concentration-absorbance graph?

    <p>Higher sensitivity at lower concentrations</p> Signup and view all the answers

    How is analytical sensitivity related to the steepness of the curves on concentration-absorbance graphs?

    <p>Low analytical sensitivity leads to steeper curves</p> Signup and view all the answers

    What is the relationship between concentration and measured absorbance typically represented as in spectrophotometry?

    <p>Curve on a graph</p> Signup and view all the answers

    How does a more sensitive instrument behave towards small variations in absorbance?

    <p>It captures small variations in absorbance at all concentrations</p> Signup and view all the answers

    What does a flat curve on a concentration-absorbance graph indicate about the analytical sensitivity?

    <p>Low analytical sensitivity</p> Signup and view all the answers

    In spectrophotometry, what does the ability of the instrument to detect and respond to changes in concentration refer to?

    <p>Analytical sensitivity</p> Signup and view all the answers

    What kind of change in absorbance does a steeper curve on a graph indicate for each incremental change in concentration?

    <p>Rapid change</p> Signup and view all the answers

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