Amino Acids & Protein Biochemistry Quiz
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Questions and Answers

Which of the following is NOT a component of an amino acid?

  • Hydrogen (correct)
  • Central carbon
  • Carboxyl group
  • Amino group
  • What is the charge of the amino group at neutral pH?

  • Positive (correct)
  • Neutral
  • Negative
  • Variable
  • Which of the following statements about amino acids is true?

  • They have a positive charge at neutral pH (correct)
  • They are achiral compounds
  • They are not building blocks of proteins
  • Their side groups are always the same
  • What is the classification of amino acids based on?

    <p>Side chain</p> Signup and view all the answers

    Which of the following is true about protein synthesis?

    <p>It involves the formation of proteins</p> Signup and view all the answers

    What type of compounds are amino acids?

    <p>Chiral compounds</p> Signup and view all the answers

    At neutral pH, what is the charge of the carboxyl group in an amino acid?

    <p>Negative</p> Signup and view all the answers

    What is the overall charge of an amino acid at neutral pH?

    <p>Neutral</p> Signup and view all the answers

    What is the primary structure of a protein?

    <p>The sequence of amino acids in the protein</p> Signup and view all the answers

    What are amino acids considered as in the context of protein synthesis?

    <p>Building blocks</p> Signup and view all the answers

    Which group determines the functional differences of amino acids?

    <p>Side-group</p> Signup and view all the answers

    What are the two categories of amino acids based on their side-group charge?

    <p>Charged or neutral</p> Signup and view all the answers

    Which type of side-chain interaction determines the polarity of an amino acid?

    <p>Hydrogen bonding</p> Signup and view all the answers

    Which type of side-chain interaction causes hydrophobic amino acids to cluster together in the center of proteins?

    <p>Van der Waals interactions</p> Signup and view all the answers

    Which type of side-chain interaction allows the formation of disulfide bonds in proteins?

    <p>Covalent bonding</p> Signup and view all the answers

    What is the primary structure of a protein?

    <p>The sequence of amino acids in a polypeptide</p> Signup and view all the answers

    Which secondary structure of a protein is stabilized by hydrogen bonds between amino acids?

    <p>α-helix</p> Signup and view all the answers

    What is the tertiary structure of a protein?

    <p>The additional folding due to interactions of side chains</p> Signup and view all the answers

    What is the quaternary structure of a protein?

    <p>The formation of more than one polypeptide</p> Signup and view all the answers

    What is the process of joining amino acids together in a protein called?

    <p>Peptide bonding</p> Signup and view all the answers

    Which of the following is true about amino acids?

    <p>Amino acids have no charge at neutral pH</p> Signup and view all the answers

    What is the classification of amino acids based on?

    <p>Amino acid structure</p> Signup and view all the answers

    What is the process of joining amino acids together in a protein called?

    <p>Protein synthesis</p> Signup and view all the answers

    What is the primary structure of a protein?

    <p>The sequence of amino acids in a protein</p> Signup and view all the answers

    Which secondary structure of a protein is stabilized by hydrogen bonds between amino acids?

    <p>Alpha helix</p> Signup and view all the answers

    What is the tertiary structure of a protein?

    <p>The overall three-dimensional shape of a protein</p> Signup and view all the answers

    What type of compounds are amino acids?

    <p>Chiral compounds</p> Signup and view all the answers

    Which type of side-chain interaction determines the polarity of an amino acid?

    <p>Ionic interactions</p> Signup and view all the answers

    What are the two categories of amino acids based on their side-group charge?

    <p>Acidic and basic</p> Signup and view all the answers

    What is the quaternary structure of a protein?

    <p>The interaction between multiple protein subunits</p> Signup and view all the answers

    Which type of side-chain interaction allows hydrophobic amino acids to cluster together in the center of proteins?

    <p>Van der Waals interactions</p> Signup and view all the answers

    Which of the following is NOT a characteristic of polar amino acids?

    <p>They have sulfur groups in their side chains</p> Signup and view all the answers

    What is the primary structure of a protein?

    <p>The sequence of amino acids in a polypeptide</p> Signup and view all the answers

    Which type of side-chain interaction determines the polarity of an amino acid?

    <p>Hydrogen bonds</p> Signup and view all the answers

    What is the charge of the amino group at neutral pH?

    <p>Positive</p> Signup and view all the answers

    What is the quaternary structure of a protein?

    <p>The interaction between multiple polypeptides</p> Signup and view all the answers

    Which type of side-chain interaction allows the formation of disulfide bonds in proteins?

    <p>Disulfide bonds</p> Signup and view all the answers

    What is the tertiary structure of a protein?

    <p>The additional folding of a protein</p> Signup and view all the answers

    Which of the following statements about amino acids is true?

    <p>Amino acids can have different side-chain structures</p> Signup and view all the answers

    What is the process of joining amino acids together in a protein called?

    <p>Peptide bond formation</p> Signup and view all the answers

    Which of the following is true about amino acids?

    <p>Amino acids have a neutral charge at neutral pH.</p> Signup and view all the answers

    What is the charge of the amino group at neutral pH?

    <p>Positive charge</p> Signup and view all the answers

    What is the charge of the carboxyl group in an amino acid at neutral pH?

    <p>Negative charge</p> Signup and view all the answers

    What is the primary structure of a protein?

    <p>The sequence of amino acids in a protein.</p> Signup and view all the answers

    What are amino acids considered as in the context of protein synthesis?

    <p>Building blocks</p> Signup and view all the answers

    What type of compounds are amino acids?

    <p>Chiral compounds</p> Signup and view all the answers

    What is the classification of amino acids based on?

    <p>Their side chain (R-group)</p> Signup and view all the answers

    Which type of side-chain interaction allows the formation of disulfide bonds in proteins?

    <p>Covalent bonds</p> Signup and view all the answers

    Which secondary structure of a protein is stabilized by hydrogen bonds between amino acids?

    <p>Alpha helix</p> Signup and view all the answers

    Which type of side-chain interaction determines the polarity of an amino acid?

    <p>Ionic interactions</p> Signup and view all the answers

    Which of the following is NOT a characteristic of polar amino acids?

    <p>They have aliphatic side chains.</p> Signup and view all the answers

    What is the charge of the carboxyl group in an amino acid at neutral pH?

    <p>Negative</p> Signup and view all the answers

    What are the two categories of amino acids based on their side-group charge?

    <p>Charged and neutral</p> Signup and view all the answers

    Which secondary structure of a protein is stabilized by hydrogen bonds between amino acids?

    <p>Alpha-helix</p> Signup and view all the answers

    What is the primary structure of a protein?

    <p>The sequence of amino acids in a polypeptide.</p> Signup and view all the answers

    What is the tertiary structure of a protein?

    <p>The additional folding due to interactions of side chains.</p> Signup and view all the answers

    What is the quaternary structure of a protein?

    <p>Proteins made from more than one polypeptide.</p> Signup and view all the answers

    What type of compounds are amino acids?

    <p>Proteins</p> Signup and view all the answers

    What type of side-chain interaction causes hydrophobic amino acids to cluster together in the center of proteins?

    <p>Van der Waals interactions</p> Signup and view all the answers

    What are amino acids considered as in the context of protein synthesis?

    <p>Building blocks</p> Signup and view all the answers

    Which of the following is true about amino acids?

    <p>Amino acids are chiral compounds.</p> Signup and view all the answers

    What is the overall charge of an amino acid at neutral pH?

    <p>Neutral</p> Signup and view all the answers

    What is the classification of amino acids based on?

    <p>Side chain (R-group)</p> Signup and view all the answers

    What type of compounds are amino acids?

    <p>Chiral compounds</p> Signup and view all the answers

    Which type of side-chain interaction determines the polarity of an amino acid?

    <p>Polar interactions</p> Signup and view all the answers

    What is the primary structure of a protein?

    <p>The sequence of amino acids</p> Signup and view all the answers

    Which secondary structure of a protein is stabilized by hydrogen bonds between amino acids?

    <p>Beta sheet</p> Signup and view all the answers

    What is the process of joining amino acids together in a protein called?

    <p>Protein synthesis</p> Signup and view all the answers

    Which type of side-chain interaction allows the formation of disulfide bonds in proteins?

    <p>Covalent interactions</p> Signup and view all the answers

    What are the two categories of amino acids based on their side-group charge?

    <p>Basic and acidic</p> Signup and view all the answers

    Which of the following is NOT a characteristic of polar amino acids?

    <p>They have aliphatic side chains.</p> Signup and view all the answers

    What is the overall charge of an amino acid at neutral pH?

    <p>Neutral</p> Signup and view all the answers

    Which type of side-chain interaction allows the formation of disulfide bonds in proteins?

    <p>Covalent bonding</p> Signup and view all the answers

    Which of the following statements about protein synthesis is true?

    <p>Peptide bonds are formed between amino acids.</p> Signup and view all the answers

    Which type of side-chain interaction causes hydrophobic amino acids to cluster together in the center of proteins?

    <p>Hydrophobic interaction</p> Signup and view all the answers

    What are the two categories of amino acids based on their side-group charge?

    <p>Neutral and charged</p> Signup and view all the answers

    Which secondary structure of a protein is stabilized by hydrogen bonds between amino acids?

    <p>Beta-sheet</p> Signup and view all the answers

    What is the primary structure of a protein?

    <p>The sequence of amino acids in a polypeptide</p> Signup and view all the answers

    What is the quaternary structure of a protein?

    <p>Proteins made from more than one polypeptide</p> Signup and view all the answers

    Which of the following statements about amino acids is true?

    <p>Amino acids can be polar or non-polar.</p> Signup and view all the answers

    Which enzyme targets neutral nonpolar amino acids during protein digestion?

    <p>Exopeptidase</p> Signup and view all the answers

    Which transporter is responsible for the absorption of dipeptides and tripeptides in the small intestine?

    <p>PEPT1</p> Signup and view all the answers

    Which amino acids have the quickest transporter affinity during protein absorption?

    <p>Met, leu, ile, val</p> Signup and view all the answers

    What is the process called when an amino group is transferred from an amino acid to a keto acid to form a new amino acid?

    <p>Transamination</p> Signup and view all the answers

    Which organ is responsible for ammonia excretion during urea synthesis?

    <p>Liver</p> Signup and view all the answers

    What are the end products of deamination?

    <p>Ammonia and keto acid</p> Signup and view all the answers

    Which of the following is NOT a function of keto acid produced during deamination?

    <p>Cholesterol</p> Signup and view all the answers

    Which expert consultation report provides information on protein and amino acid requirements in human nutrition?

    <p>WHO/FAO/UNU Expert Consultation</p> Signup and view all the answers

    What is the URL of the WHO Technical Report Series on protein and amino acid requirements in human nutrition?

    <p><a href="http://whqlibdoc.who.int/trs/WHO_TRS_935_eng">http://whqlibdoc.who.int/trs/WHO_TRS_935_eng</a></p> Signup and view all the answers

    What is the full name of the author of the lecture on protein digestion and metabolism?

    <p>Aifric O’Sullivan BSc, MSc, PhD</p> Signup and view all the answers

    Which enzyme is responsible for the hydrolysis of proteins into peptides during protein digestion?

    <p>Endopeptidase</p> Signup and view all the answers

    Which transporter is responsible for the absorption of dipeptides and tripeptides in the small intestine?

    <p>PEPT1 (H+)</p> Signup and view all the answers

    Which amino acids have the slowest transporter affinity during protein absorption?

    <p>Glutamate and Aspartate</p> Signup and view all the answers

    Which process involves the transfer of an amino group from an amino acid to a keto acid to form a new amino acid?

    <p>Transamination</p> Signup and view all the answers

    Which amino acid is formed from the transamination of glutamate and pyruvate?

    <p>Alanine</p> Signup and view all the answers

    Which organ is responsible for the excretion of ammonia during urea synthesis?

    <p>Liver</p> Signup and view all the answers

    Which expert consultation report provides information on protein and amino acid requirements in human nutrition?

    <p>WHO/FAO/UNU Expert Consultation</p> Signup and view all the answers

    Which amino acids are classified as dispensable?

    <p>Asp, Glu, Ser, Gly</p> Signup and view all the answers

    What are the two categories of amino acids based on their side-group charge?

    <p>Neutral and Charged</p> Signup and view all the answers

    What is the overall charge of an amino acid at neutral pH?

    <p>Neutral</p> Signup and view all the answers

    Which type of process rheometer measurement is considered the most desirable?

    <p>In-line</p> Signup and view all the answers

    What is the critical shear stress that causes the onset of flow called?

    <p>Yield stress</p> Signup and view all the answers

    What is the deformation resulting from the applied stress, at which the flow starts, called?

    <p>Shear deformation</p> Signup and view all the answers

    Which type of fluid has the same viscosity at low shear rates as at high shear rates?

    <p>Newtonian fluid</p> Signup and view all the answers

    Which type of fluid has a nonlinear and sometimes time-dependent relation between shear stress and strain rate?

    <p>Non-Newtonian fluid</p> Signup and view all the answers

    Which of the following is NOT a type of probe used in process control for measuring processing conditions and product quality parameters?

    <p>Rheometers</p> Signup and view all the answers

    Which of the following sensors is used to measure the density of liquid food by vibrating like a tuning fork?

    <p>Density sensors</p> Signup and view all the answers

    Which of the following is NOT a property measured by viscometers in rheology?

    <p>Texture</p> Signup and view all the answers

    Which of the following is a challenge in process rheometry for in-line measurement?

    <p>Periodic sampling is required</p> Signup and view all the answers

    What does rheology study in relation to foods?

    <p>Deformation and flow</p> Signup and view all the answers

    What type of viscometer consists of a probe vibrating at a known frequency and amplitude to measure viscosity?

    <p>Vibrational viscometer</p> Signup and view all the answers

    Which viscometer is used for highly viscous materials that would not flow into the annular space of a rotational viscometer?

    <p>Cone and plate</p> Signup and view all the answers

    Which viscometer measures viscosity by allowing a piston or cylinder to fall through the process liquid within a tube and measuring the time of fall?

    <p>Falling Cylinder</p> Signup and view all the answers

    Which viscometer measures viscosity by measuring the terminal velocity of a falling sphere through a liquid?

    <p>Falling Sphere Viscometer</p> Signup and view all the answers

    Which viscometer uses a funnel flow from a beaker or cup to measure viscosity?

    <p>Funnel flow from beaker or cup</p> Signup and view all the answers

    Which of the following factors does NOT influence the viscosity of skim milk concentrate?

    <p>pH level</p> Signup and view all the answers

    What is the recommended viscosity for skim milk concentrate in falling film evaporators?

    <p>100 mPa/s at 100/s shear rate</p> Signup and view all the answers

    Which energy source is primarily used in spray dryers to remove water from skim milk concentrate?

    <p>Natural gas</p> Signup and view all the answers

    Which of the following is true about protein content in skim milk during the months of April/May and June/July?

    <p>Protein content is higher in April/May and lower in June/July</p> Signup and view all the answers

    What is the primary objective of continuous monitoring of skim milk concentrate viscosity during skim milk production?

    <p>To prevent blockage in pipelines</p> Signup and view all the answers

    Which equation represents the relationship between shear stress and shear rate in the power law model?

    <p>$\tau = \eta \cdot \gamma^n$</p> Signup and view all the answers

    Which model is commonly used to describe the flow behavior of molten chocolate?

    <p>Casson model</p> Signup and view all the answers

    Which equation represents the relationship between shear stress and shear rate in the Casson model?

    <p>$\tau = \tau_0 + \eta \cdot \gamma^{0.5}$</p> Signup and view all the answers

    Which model is a generalized model of a non-Newtonian fluid?

    <p>Herschel-Bulkley model</p> Signup and view all the answers

    Which equation represents the relationship between shear stress and shear rate in the Herschel-Bulkley model?

    <p>$\tau = \tau_0 + K \cdot \gamma^n$</p> Signup and view all the answers

    According to Stoke's law, the terminal velocity (vt) of a spherical particle in a fluid is given by the equation $vt = \frac{{d^2(\rho_s - \rho)g}},{{18\mu}}$, where d is the diameter of the particle, ρs is the density of the sphere, ρ is the density of the liquid, g is the acceleration due to gravity, and μ is the viscosity of the fluid. If the diameter of the particle is 0.01 m, the density of the sphere is 1000 kg/m3, the density of the liquid is 800 kg/m3, the acceleration due to gravity is 9.81 m/s2, and the viscosity of the fluid is $0.01 Ns/m^2$, what is the terminal velocity of the particle?

    <p>0.1 m/s</p> Signup and view all the answers

    According to Stoke's equation, the settling area (A) in a tank can be calculated using the equation $A = \frac{{F \cdot (dw/dt)}},{{vr}}$, where F is the mass ratio of liquid to solid in the feed, dw/dt is the mass rate of feed of the solids, v is the velocity, and r is the density of the liquid. If F is 2, dw/dt is 500 kg/h, v is 1 m/s, and r is 1000 kg/m3, what is the settling area?

    <p>0.5 m2</p> Signup and view all the answers

    According to Stoke's equation, the terminal velocity (vt) of a spherical particle in a fluid can also be calculated using the equation $vt = \frac{{d^2(\rho_s - \rho)g}},{{18\mu}}$. If the terminal velocity of a particle is 0.1 m/s, the diameter of the particle is 0.02 m, the density of the sphere is 1200 kg/m3, the density of the liquid is 900 kg/m3, and the viscosity of the fluid is $0.02 Ns/m^2$, what is the acceleration due to gravity (g)?

    <p>9.8 m/s2</p> Signup and view all the answers

    According to Stoke's equation, the terminal velocity (vt) of a spherical particle in a fluid can be calculated using the equation $vt = \frac{{d^2(\rho_s - \rho)g}},{{18\mu}}$. If the terminal velocity of a particle is 0.05 m/s, the diameter of the particle is 0.03 m, the density of the sphere is 1500 kg/m3, the density of the liquid is 1000 kg/m3, and the viscosity of the fluid is $0.03 Ns/m^2$, what is the terminal velocity in cm/h?

    <p>2 cm/h</p> Signup and view all the answers

    According to Stoke's equation, the terminal velocity (vt) of a spherical particle in a fluid can be calculated using the equation $vt = \frac{{d^2(\rho_s - \rho)g}},{{18\mu}}$. If the terminal velocity of a particle is 0.02 m/s, the diameter of the particle is 0.04 m, the density of the sphere is 1800 kg/m3, the density of the liquid is 1200 kg/m3, and the viscosity of the fluid is $0.04 Ns/m^2$, what is the terminal velocity in mm/s?

    <p>60 mm/s</p> Signup and view all the answers

    Which of the following is NOT an attribute related to chromatic colour measurement?

    <p>Geometric</p> Signup and view all the answers

    What is the better approach to define colour in food manufacturing operations?

    <p>Objectively</p> Signup and view all the answers

    What is the Munsell system used for in relation to colour measurement?

    <p>To compare colours visually</p> Signup and view all the answers

    What are the limitations of using the Munsell system for colour measurement?

    <p>All of the above</p> Signup and view all the answers

    What have been developed to overcome the limitations of the Munsell system in colour measurement?

    <p>Objective measurements</p> Signup and view all the answers

    What is the relationship between a change in one attribute and the perceived change in colour?

    <p>Direct</p> Signup and view all the answers

    What are the two attributes related to colour measurement?

    <p>Geometric and chromatic</p> Signup and view all the answers

    Which illuminant represents light from an incandescent lamp?

    <p>Illuminant A</p> Signup and view all the answers

    What is the color temperature of Illuminant B?

    <p>4800 K</p> Signup and view all the answers

    Which illuminant represents average daylight from the entire sky?

    <p>Illuminant C</p> Signup and view all the answers

    What is the color temperature of Illuminant C?

    <p>6500 K</p> Signup and view all the answers

    What is the purpose of the CIE 20 standard observation curves?

    <p>To quantify the average response of the human eye to red, green, and blue</p> Signup and view all the answers

    Which instrument can be used to obtain X, Y, and Z values for color measurement?

    <p>All of the above</p> Signup and view all the answers

    Which color scale is based on opponent colors theory?

    <p>Hunter Lab color scale</p> Signup and view all the answers

    Which method is used for color determination of beer and worts?

    <p>Beer tintometer</p> Signup and view all the answers

    What is the final color trimming achieved by in whiskey production?

    <p>Caramel coloring</p> Signup and view all the answers

    What is the wavelength of light used for color determination in whiskey production?

    <p>525 nm</p> Signup and view all the answers

    What instrument is used for color determination of cider?

    <p>Minolta colorimeter</p> Signup and view all the answers

    What parameters are set by the laboratory for color determination of cider?

    <p>Tristimulus reading</p> Signup and view all the answers

    What offers more flexibility in range of beverages for color determination than a Lovibond colorimeter?

    <p>Minolta colorimeter</p> Signup and view all the answers

    What is the recommended bandwidth for the spectrometer used in color determination of whiskey?

    <p>Less than 10 nm</p> Signup and view all the answers

    What is the internationally recognized method used for instrumentally measuring color in whiskey?

    <p>Association of Analytical Chemist method</p> Signup and view all the answers

    What is the purpose of the Lovibond discs in color determination of beer and worts?

    <p>Final color trimming</p> Signup and view all the answers

    What is the purpose of representing a light source as a function in color quantification?

    <p>To determine the spectral characteristics of different light sources</p> Signup and view all the answers

    What is the function of the cones in the human eye?

    <p>To provide color vision</p> Signup and view all the answers

    What is the purpose of the CIE x, y, z standard observer curves?

    <p>To plot the human response to color against wavelength</p> Signup and view all the answers

    What is the purpose of using XYZ reference stimuli in color quantification?

    <p>To define a mathematical concept of color</p> Signup and view all the answers

    What is the process of measuring color using reflection spectrophotometry?

    <p>Shining red, green, and blue light beams onto a half circle and matching the color produced to a standard</p> Signup and view all the answers

    What is the primary function of the rods in the human eye?

    <p>To record light and darkness</p> Signup and view all the answers

    What is the purpose of representing the amount of reflected or transmitted light at each wavelength as a spectral curve?

    <p>To quantify the reflectance or transmittance of an object at different wavelengths</p> Signup and view all the answers

    What is the most biologically active form of vitamin E?

    <p>α-tocopherol</p> Signup and view all the answers

    What is the equation for calculating α-tocopherol equivalents?

    <p>$ ext{α-tocopherol Equivalents} = mg α-tocopherol + 0.5 x mg β-tocopherol + 0.1 x mg γ-tocopherol + 0.3 x mg α-tocotrienol$</p> Signup and view all the answers

    What is the α-tocopherol equivalent content of 1 ounce of hazelnuts?

    <p>3.1 mg</p> Signup and view all the answers

    What is the α-tocopherol equivalent content of 1 tablespoon of sunflower oil?

    <p>8.3 mg</p> Signup and view all the answers

    What is the primary function of dietary antioxidants?

    <p>Neutralize free radicals and donate an electron</p> Signup and view all the answers

    What is the main function of Vitamin E in the body?

    <p>Limit radical damage from oxidation of PUFAs</p> Signup and view all the answers

    What is the association between high blood concentrations of vitamin E and cardiovascular disease (CVD)?

    <p>Lower incidence of CVD</p> Signup and view all the answers

    What can high levels of free radicals cause in the body?

    <p>Cellular dysfunction and disease</p> Signup and view all the answers

    Which vitamins are considered dietary antioxidants?

    <p>Vitamin C, Vitamin E, Beta-Carotene</p> Signup and view all the answers

    What is the role of cofactors and enzymes in defending against disease?

    <p>Interconnection between many micronutrients</p> Signup and view all the answers

    What is the impact of low antioxidant intake in the diet?

    <p>Increased oxidative stress and disease</p> Signup and view all the answers

    What are the potential consequences of exposure to toxins, poor nutrition, and stress?

    <p>Oxidative stress and disease</p> Signup and view all the answers

    What is the main effect of age-related decline in natural defense mechanisms?

    <p>Increased oxidative stress</p> Signup and view all the answers

    What is the overall impact of high levels of free radicals on cells?

    <p>Cellular dysfunction and disease</p> Signup and view all the answers

    What is the primary function of VLDL in the body?

    <p>Transport lipids to other tissues</p> Signup and view all the answers

    What is the main function of tocopherol in the body?

    <p>Limit radical damage from oxidation of PUFAs</p> Signup and view all the answers

    What is the primary function of dietary antioxidants?

    <p>Neutralize free radicals</p> Signup and view all the answers

    What do free radicals damage within the body?

    <p>Cell membranes, DNA, RNA, and proteins</p> Signup and view all the answers

    What is the primary purpose of vitamin E in the body?

    <p>Limit radical damage from oxidation of PUFAs</p> Signup and view all the answers

    What is the association between high blood concentrations of vitamin E and cardiovascular disease (CVD)?

    <p>Lower incidence of CVD</p> Signup and view all the answers

    What are examples of dietary antioxidants?

    <p>Vitamin C, Vitamin E, Beta-Carotene</p> Signup and view all the answers

    What can exposure to toxins, poor nutrition, and stress lead to?

    <p>Oxidative stress and increased disease</p> Signup and view all the answers

    What are the potential health risks associated with high levels of free radicals?

    <p>Cancer, Cardiovascular Disease, Cataracts</p> Signup and view all the answers

    What is the impact of randomised controlled trials with high dose vitamin E supplements?

    <p>Small effect or no effect</p> Signup and view all the answers

    What is the overall impact of low antioxidant intake in the diet?

    <p>Increase in oxidative stress and disease risk</p> Signup and view all the answers

    What is the role of vitamin C in the reduction of vitamin E radical back to tocopherol?

    <p>Reaction with vitamin E radical to reduce it back to tocopherol</p> Signup and view all the answers

    What is the effect of high levels of free radicals on cells?

    <p>Damage to cells and contribution to disease</p> Signup and view all the answers

    What do antioxidants remain stable and do in the body?

    <p>Donate an electron to neutralize free radicals</p> Signup and view all the answers

    Which of the following is a symptom of vitamin A deficiency?

    <p>Xerophthalmia</p> Signup and view all the answers

    What is the direct measure used to assess vitamin A status?

    <p>Liver biopsy</p> Signup and view all the answers

    What is the main function of retinoic acid in the body?

    <p>Regulates gene expression and cell differentiation</p> Signup and view all the answers

    What is the consequence of excessive intake of vitamin A?

    <p>Accumulation in liver and other tissues</p> Signup and view all the answers

    Which receptors bind to retinoic acid response elements (RARE) on DNA?

    <p>α,β,γ retinoic acid receptor (RAR) and α,β,γ retinoic X receptor (RXR)</p> Signup and view all the answers

    What is the primary function of carotenoids in the body?

    <p>Act as antioxidants</p> Signup and view all the answers

    What is the recommended upper limit of dietary intake for vitamin A in pregnant women to avoid teratogenic effects?

    <p>3000-3300 µg</p> Signup and view all the answers

    What is the method used to measure the relative change in serum concentration after vitamin A ingestion?

    <p>Relative Dose Response test</p> Signup and view all the answers

    What is the consequence of vitamin A deficiency on vision?

    <p>Night blindness</p> Signup and view all the answers

    What is the primary role of retinoic acid in gene expression?

    <p>Activates transcription</p> Signup and view all the answers

    What is the consequence of vitamin A deficiency on the eye?

    <p>Bitot’s spots (small white cell accumulation)</p> Signup and view all the answers

    What is the impact of vitamin A toxicity on the liver and bone?

    <p>Damage and accumulation</p> Signup and view all the answers

    What is the recommended dietary allowance (RDA) of vitamin A for men and women?

    <p>Men = 700 µg, Women = 600 µg</p> Signup and view all the answers

    What is the primary storage site for vitamin A in the body?

    <p>Liver and adipose tissue</p> Signup and view all the answers

    What is the biological activity of β-carotene compared to retinol?

    <p>~ 12µg = 1µg retinol/RAE</p> Signup and view all the answers

    What is the primary function of vitamin A in the body?

    <p>Vision, growth, reproduction, and immune function</p> Signup and view all the answers

    What is the formula to calculate Retinol Activity Equivalent (RAE) for carotenoids?

    <p>RAE = 1µg retinol</p> Signup and view all the answers

    What is the recommended dietary allowance (RDA) of vitamin A for lactating women?

    <p>950 µg</p> Signup and view all the answers

    What is the primary metabolic function of vitamin A?

    <p>Participates in the conversion of light into nerve impulses</p> Signup and view all the answers

    What are the end products of the cleavage of ProVitamin A?

    <p>Retinoic acid</p> Signup and view all the answers

    What is the primary source of Vitamin A in the diet?

    <p>Beef liver</p> Signup and view all the answers

    Which proteins are involved in the transport and storage of vitamin A in the body?

    <p>Retinol Binding Protein and transthyretin</p> Signup and view all the answers

    What is the primary function of vitamin A in vision?

    <p>Maintains the cornea</p> Signup and view all the answers

    What is the recommended dietary allowance (RDA) of vitamin A for children aged 7-10?

    <p>600 µg</p> Signup and view all the answers

    What percentage of the body's calcium is found in bones and teeth?

    <p>99%</p> Signup and view all the answers

    What is the recommended dietary allowance (RDA) of calcium for adults?

    <p>1200mg</p> Signup and view all the answers

    Which physiological factor influences calcium absorption?

    <p>Vitamin D status</p> Signup and view all the answers

    Which food contributes approximately 200mg of calcium to the diet, according to the text?

    <p>125mls milk</p> Signup and view all the answers

    What is the percentage of calcium absorption that is concentration dependent?

    <p>30%</p> Signup and view all the answers

    What is the primary role of vitamin D in calcium metabolism?

    <p>Stimulates calcium absorption</p> Signup and view all the answers

    What is the plasma calcium concentration range in milligrams per 100 milliliters?

    <p>9-10.4 mg/100 ml</p> Signup and view all the answers

    Which dietary factor can inhibit calcium absorption, as mentioned in the text?

    <p>Oxalates and phytates in foods</p> Signup and view all the answers

    What is the approximate calcium content in 1.5 cartons of cottage cheese?

    <p>300mg</p> Signup and view all the answers

    What is the approximate calcium content in a serving of canned sardines (56g)?

    <p>400mg</p> Signup and view all the answers

    How much calcium is deposited in the body with the help of vitamin D?

    <p>200mg</p> Signup and view all the answers

    What is the approximate calcium content in 6 medium slices of white or brown bread?

    <p>200mg</p> Signup and view all the answers

    Which hormone signals the release of calcitonin in response to rising blood calcium levels?

    <p>Vitamin D</p> Signup and view all the answers

    What is the normal range for plasma calcium concentration in milligrams per 100 milliliters?

    <p>9-10.4</p> Signup and view all the answers

    What is the primary metabolic function of calcium in addition to bone and teeth health?

    <p>Assisting in blood clotting</p> Signup and view all the answers

    What is the World Health Organization's definition of osteopenia based on bone mineral density?

    <p>BMD between -1 and -2.5 SD of the young adult mean</p> Signup and view all the answers

    What is the upper limit for daily calcium intake according to the US?

    <p>2500 mg/day</p> Signup and view all the answers

    What is a potential consequence of calcium deficiency?

    <p>Osteoporosis</p> Signup and view all the answers

    What is the primary storage site for vitamin A in the body?

    <p>Liver</p> Signup and view all the answers

    What is the primary impact of high levels of free radicals on cells?

    <p>Cell damage and aging</p> Signup and view all the answers

    What is the overall impact of low antioxidant intake in the diet?

    <p>Increased oxidative stress</p> Signup and view all the answers

    What is the function of the cones in the human eye?

    <p>Detecting color and detail</p> Signup and view all the answers

    Which transporter is responsible for the absorption of dipeptides and tripeptides in the small intestine?

    <p>PEPT1</p> Signup and view all the answers

    What is the primary metabolic function of vitamin A?

    <p>Supporting vision and immune function</p> Signup and view all the answers

    What is the primary difference between magnification and resolution in the context of light microscopy?

    <p>Magnification refers to the degree of image enlargement, while resolution refers to the amount of detail visible in the image.</p> Signup and view all the answers

    What is the resolution limit of a light microscope?

    <p>0.2µm</p> Signup and view all the answers

    Which type of microscope has a higher magnification compared to a light microscope?

    <p>Electron microscope</p> Signup and view all the answers

    What is the consequence of attempting to use increased magnification to improve the resolution of a light microscope?

    <p>The resolution will not improve, as magnification does not affect resolution in light microscopy.</p> Signup and view all the answers

    What type of microscopy is used to observe external features of cells?

    <p>Light Microscopy</p> Signup and view all the answers

    What is the primary difference between prokaryotic and eukaryotic cells?

    <p>Presence of a nucleus</p> Signup and view all the answers

    How do most electron microscopes magnify objects?

    <p>Up to 1 million times</p> Signup and view all the answers

    What is the primary method of eukaryotic cell division?

    <p>Mitosis</p> Signup and view all the answers

    What is the main function of light microscopes in observing cells?

    <p>See living cells in action</p> Signup and view all the answers

    What is the primary difference in the cytoplasmic membrane of eukaryotic and prokaryotic cells?

    <p>Presence of sterols</p> Signup and view all the answers

    What is the primary difference between scanning electron microscopy and transmission electron microscopy?

    <p>Observation of external vs internal cell features</p> Signup and view all the answers

    What provides 'illumination' in electron microscopy with a shorter wavelength than light photons?

    <p>Electrons</p> Signup and view all the answers

    What is the primary difference in cell division between prokaryotic and eukaryotic cells?

    <p>Binary Fission vs Mitosis</p> Signup and view all the answers

    What is the primary difference in the structure of prokaryotic and eukaryotic cells' nucleic acid?

    <p>Presence of a nuclear membrane</p> Signup and view all the answers

    What is the primary difference in the lenses used in electron microscopy and light microscopy?

    <p>Electromagnets vs Light</p> Signup and view all the answers

    What enables observation of internal cell structures in electron microscopy?

    <p>Transmission Electron Microscopy</p> Signup and view all the answers

    Which staining method is considered the most important in Microbiology?

    <p>Crystal Violet</p> Signup and view all the answers

    Which type of microorganism is known for having an outer membrane in its cell structure?

    <p>Gram-negative bacteria</p> Signup and view all the answers

    Which type of microscopy is used to study the detailed internal structure of microbial cells?

    <p>Transmission electron microscopy</p> Signup and view all the answers

    Which type of cells are known for their lack of a true nucleus?

    <p>Gram-positive bacteria</p> Signup and view all the answers

    Which component is stained by the first step of the Gram stain mechanism?

    <p>Cell wall</p> Signup and view all the answers

    Which type of microscopy is suitable for observing the surface details of microbial cells?

    <p>Scanning electron microscopy</p> Signup and view all the answers

    Which type of microorganism is known for its ability to thrive in extreme environments?

    <p>Archaea</p> Signup and view all the answers

    What is the primary function of the outer membrane in Gram-negative bacteria?

    <p>Regulation of nutrient uptake</p> Signup and view all the answers

    Which type of staining is used to differentiate between Gram-positive and Gram-negative bacteria?

    <p>Gram staining</p> Signup and view all the answers

    Which type of cell structure is characterized by the presence of peptidoglycan in its cell wall?

    <p>Gram-positive bacteria</p> Signup and view all the answers

    Which type of microscopy is commonly used in microbiology laboratories for routine observation of microbial cells?

    <p>Light microscopy</p> Signup and view all the answers

    Which type of cells are known for their lack of membrane-bound organelles?

    <p>Gram-positive bacteria</p> Signup and view all the answers

    What is the primary function of the bacterial cell wall?

    <p>To regulate osmotic pressure within the cell</p> Signup and view all the answers

    Which component makes up the outer membrane of Gram-negative bacteria?

    <p>Phospholipid-Lipopolysaccharide (LPS) Bilayer</p> Signup and view all the answers

    What is the main function of flagella in bacteria?

    <p>To enable motility and chemotaxis</p> Signup and view all the answers

    Which bacteria are stained using the Ziehl-Neelsen stain due to their high wax content in the cell envelope?

    <p>Mycobacteria</p> Signup and view all the answers

    What is the primary component of the bacterial cell wall that gives the cell its characteristic shape and prevents osmotic lysis?

    <p>Peptidoglycan</p> Signup and view all the answers

    Which domain of life lacks peptidoglycan in their cell wall?

    <p>Archaea</p> Signup and view all the answers

    What is the main function of the cytoplasmic membrane in bacterial cells?

    <p>Site of energy production and transport of important molecules</p> Signup and view all the answers

    What is the composition of the cell wall in Mycoplasmas, the smallest known bacteria?

    <p>They have no cell wall</p> Signup and view all the answers

    What is the purpose of the crystal violet-iodine complex in the Gram staining mechanism?

    <p>Forms within the cells to create a blue color</p> Signup and view all the answers

    What is the primary difference in the cell envelope between Gram-positive and Gram-negative bacteria?

    <p>Gram-positive has many peptidoglycan layers, while Gram-negative has one peptidoglycan layer</p> Signup and view all the answers

    Which bacterial shape is characterized as curved or spiral shaped?

    <p>Spirilla</p> Signup and view all the answers

    What is the primary function of pili in bacteria?

    <p>Adherence and plasmid transfer</p> Signup and view all the answers

    What is the result of mitosis and cytokinesis in eukaryotic cells?

    <p>Two genetically identical daughter cells</p> Signup and view all the answers

    What is the diploid chromosome number maintained in during mitosis?

    <p>2n</p> Signup and view all the answers

    Which type of cells undergo mitotic cell division?

    <p>Both animal and plant cells</p> Signup and view all the answers

    What is the purpose of mitosis in reproduction, growth, and tissue repair?

    <p>To maintain the same number of chromosomes</p> Signup and view all the answers

    What is the significance of mitosis in eukaryotic cells?

    <p>To maintain the same number of chromosomes, ensuring genetic consistency</p> Signup and view all the answers

    What is the primary function of mitosis in multicellular organisms?

    <p>To allow for growth and development of zygote after fertilization</p> Signup and view all the answers

    During which stage of mitosis do sister chromatids align along the metaphase plate?

    <p>Metaphase</p> Signup and view all the answers

    What is the result of mitosis in terms of chromosome number?

    <p>A conservation of chromosome number to the original number</p> Signup and view all the answers

    What is the primary difference between mitosis and binary fission?

    <p>The type of cells that undergo the process</p> Signup and view all the answers

    What is the process of nuclear division that occurs in eukaryotes, resulting in four non-identical daughter cells?

    <p>Meiosis</p> Signup and view all the answers

    What is the term for a pair of homologous chromosomes, each consisting of one maternal and one paternal chromatid?

    <p>Nonsister chromatids</p> Signup and view all the answers

    During which stage of meiosis do homologous chromosomes separate into 2 daughter cells?

    <p>Anaphase I</p> Signup and view all the answers

    What is the result of the replication of the genome in meiosis, where homologous chromosomes align to form a tetrad?

    <p>Tetrad formation</p> Signup and view all the answers

    How many individual phases are there in Meiosis I?

    <p>4</p> Signup and view all the answers

    What is the term for the process of cell division that results in four daughter cells, each with half the number of chromosomes as the parent cell?

    <p>Meiosis</p> Signup and view all the answers

    What is the primary purpose of DNA replication in the S-phase of interphase?

    <p>To ensure equal distribution of chromosomes during mitosis</p> Signup and view all the answers

    What is the term for a multinucleated cell generated from multiple nuclear divisions without cytokinesis?

    <p>Coenocyte</p> Signup and view all the answers

    What is the stage of the cell cycle where the chromosomes condense before mitotic nuclear division?

    <p>After genomic DNA duplication</p> Signup and view all the answers

    What is the result of incomplete or erroneous DNA replication?

    <p>Mutation</p> Signup and view all the answers

    What is the term for the phase of the cell cycle where the cell is not dividing?

    <p>G0 phase</p> Signup and view all the answers

    Who developed the chain termination method for DNA sequencing?

    <p>Frederick Sanger</p> Signup and view all the answers

    What is the molecule that carries genetic information in eukaryotic organisms?

    <p>DNA</p> Signup and view all the answers

    What is the name of the genome editing technology developed by Jennifer Doudna and Emmanuelle Charpentier?

    <p>CRISPR-Cas9</p> Signup and view all the answers

    What was the approximate cost of sequencing a mammalian genome in 2001?

    <p>€100,000,000</p> Signup and view all the answers

    What is the name of the system of units used to measure length in biology?

    <p>International System of Units (SI)</p> Signup and view all the answers

    What is the term for one million base pairs in genome size?

    <p>Mb</p> Signup and view all the answers

    Who are the scientists credited with discovering the structure of DNA?

    <p>James Watson and Francis Crick</p> Signup and view all the answers

    What is the term for the complete set of genetic material in an organism?

    <p>Genome</p> Signup and view all the answers

    Who generated the X-ray crystallography data that was crucial to Watson and Crick’s 3D model of the structure of DNA?

    <p>Rosalind Franklin</p> Signup and view all the answers

    What is the process of translating genetic information encoded in DNA/RNA into amino acid sequences?

    <p>Translation</p> Signup and view all the answers

    What is the term for the thread-like structures that carry genetic information in the nucleus of eukaryotic cells?

    <p>Chromosomes</p> Signup and view all the answers

    What is the term for the process of joining amino acids together to form a protein?

    <p>Protein synthesis</p> Signup and view all the answers

    In Hindu religion, what was believed to be passed on from parents to offspring?

    <p>Sickness</p> Signup and view all the answers

    Who published the book 'On the Origin of Species by Means of Natural Selection' in 1859?

    <p>Charles Darwin</p> Signup and view all the answers

    Who isolated 'nuclein' (nucleic acid - DNA) from the nuclei of white blood cells in 1869?

    <p>Friedrich Miescher</p> Signup and view all the answers

    Who demonstrated that genes are located on specific chromosomes in 1910?

    <p>Thomas Hunt Morgan</p> Signup and view all the answers

    Who elucidated the three-dimensional structure of DNA (the double helix) in 1953?

    <p>James Watson and Francis Crick</p> Signup and view all the answers

    What did Oswald Avery, Colin MacLeod, and Maclyn McCarty demonstrate in 1944?

    <p>That DNA is the genetic material</p> Signup and view all the answers

    What is the purpose of microinjection of foreign DNA into embryo pronuclei?

    <p>To produce a transgenic animal with a desired trait</p> Signup and view all the answers

    What is the role of the promoter in generating transgenic animals?

    <p>To target the expression of the gene of interest to a specific tissue</p> Signup and view all the answers

    What is the purpose of superovulating an egg donor in generating transgenic animals?

    <p>To produce multiple ovulations</p> Signup and view all the answers

    What is the outcome of nuclear transfer in generating transgenic animals?

    <p>Replacement of the oocyte nucleus with a diploid donor cell nucleus</p> Signup and view all the answers

    What is the percentage of transgenic offspring produced using microinjection of DNA into the pronucleus?

    <p>1-5%</p> Signup and view all the answers

    What is the key advantage of using nuclear transfer in producing transgenic animals?

    <p>It allows for the use of cultured transgenic cells as nuclear donors with 100% transgenic efficiency</p> Signup and view all the answers

    What is the function of tissue plasminogen activator (TPA) produced by transgenic animals?

    <p>Prevents strokes by dissolving blood clots</p> Signup and view all the answers

    Which of the following procedures does NOT involve genetic engineering?

    <p>Surrogacy</p> Signup and view all the answers

    What is the name of the first cloned mammal produced using nuclear transfer technology?

    <p>Dolly the sheep</p> Signup and view all the answers

    What is the function of human growth factor produced by transgenic animals?

    <p>Treats pituitary dwarfism</p> Signup and view all the answers

    What is the main difference between traditional selective breeding and genetic engineering in plants?

    <p>Genetic engineering allows for the introduction of genes from unrelated species.</p> Signup and view all the answers

    What is the role of Agrobacterium tumefaciens in plant genetic engineering?

    <p>It is a soil bacterium that causes crown galls and integrates the T DNA into plant genomes.</p> Signup and view all the answers

    What is the characteristic of Dolly that makes her not transgenic?

    <p>She does not carry a gene from a different species in her genome.</p> Signup and view all the answers

    What is the advantage of using recombinant DNA technology in plant genetic engineering?

    <p>It overcomes the limitations of traditional selective breeding by allowing the introduction of genes from unrelated species.</p> Signup and view all the answers

    What is the role of the Ti plasmid in plant genetic engineering?

    <p>It is a small section of the Agrobacterium tumefaciens genome that integrates into plant genomes.</p> Signup and view all the answers

    What is the primary purpose of DNA cloning in genetic engineering?

    <p>To create identical copies of a gene for pharmaceutical production</p> Signup and view all the answers

    What is a characteristic of plasmids used in DNA cloning?

    <p>They can range in size from 1 kb to 200 kb</p> Signup and view all the answers

    What is a major challenge in examining single genes from a genome of interest?

    <p>Naturally-occurring DNA is very long and can include many discrete genes</p> Signup and view all the answers

    What is the term for any DNA molecule that has been generated in vitro and contains DNA sequences from two or more distinct DNA molecules?

    <p>Recombinant DNA</p> Signup and view all the answers

    What is the purpose of DNA extraction and purification in genetic engineering?

    <p>To prepare DNA for cloning and genetic modification</p> Signup and view all the answers

    What is the primary application of DNA biotechnology in agricultural studies?

    <p>Development of insect-resistant crops</p> Signup and view all the answers

    What is the significance of the 1996 milestone in the timeline of biotechnology?

    <p>Cloning of the first mammal</p> Signup and view all the answers

    What is the primary goal of bioremediation, a significant application of biotechnology?

    <p>Remediation of environmental pollutants</p> Signup and view all the answers

    What is the significance of Paul Berg's Nobel prize in 1980?

    <p>Development of a general method for joining two DNAs together in vitro</p> Signup and view all the answers

    What is the primary application of gene therapy, a significant aspect of biotechnology?

    <p>Treatment of genetic disorders</p> Signup and view all the answers

    What is the advantage of nuclear transfer over pronuclear microinjection in creating transgenic animals?

    <p>Resulting animals have the same nuclear genome as the donor cells plus the transgene</p> Signup and view all the answers

    What is the purpose of superovulating an egg donor in the process of creating transgenic animals?

    <p>To increase the number of oocytes available for microinjection</p> Signup and view all the answers

    What is the advantage of using transgenic animals for pharmaceutical production?

    <p>Can isolate large quantities of protein from milk</p> Signup and view all the answers

    What is the purpose of clonal selection in the process of creating transgenic animals?

    <p>To select for transgenic cells</p> Signup and view all the answers

    What is the result of microinjection of a transgene into a pig oocyte?

    <p>Transgenic offspring with the transgene expressed in mammary tissue</p> Signup and view all the answers

    What is the primary application of transgenic plants with herbicide resistance?

    <p>To reduce pesticide use</p> Signup and view all the answers

    What is the purpose of introducing the Ti plasmid into plant cells?

    <p>To create a transgenic plant with a new trait</p> Signup and view all the answers

    What is an example of a transgenic plant with a nutraceutical application?

    <p>Golden Rice</p> Signup and view all the answers

    What is a potential application of transgenic plants with abiotic stress tolerance?

    <p>Increased crop yield</p> Signup and view all the answers

    What is the role of Agrobacterium tumefaciens in plant genetic engineering?

    <p>To introduce a new gene into a plant cell</p> Signup and view all the answers

    What is an example of a transgenic plant with a post-harvest quality improvement?

    <p>Improved gluten quality in wheat</p> Signup and view all the answers

    What is the purpose of using restriction enzymes in plant genetic engineering?

    <p>To cut the DNA at specific sites</p> Signup and view all the answers

    What is an example of a transgenic plant with an agronomic trait?

    <p>Glyphosate-resistant soybeans</p> Signup and view all the answers

    What is the role of DNA ligase in plant genetic engineering?

    <p>To join the new gene to the plant DNA</p> Signup and view all the answers

    What is the purpose of regenerating whole plants from transgenic plant cells?

    <p>To obtain transgenic plants with a new trait</p> Signup and view all the answers

    Study Notes

    Microscopy and Cell Structures

    • Electron Microscopy uses electrons instead of light photons to image cells and cell structures
    • Electrons provide "illumination" with a shorter wavelength than light photons
    • Electron Microscope uses electromagnets as lenses and functions in a vacuum
    • Electron Microscopy involves speeding up electrons in a vacuum until their wavelength is extremely short
    • Most electron microscopes can magnify objects up to 1 million times
    • Scanning electron microscopy is used to observe external features of cells, while transmission electron microscopy is used to observe internal cell structures
    • Light microscopes enable the user to see living cells in action
    • New strategies involving video cameras, polarized light, fluorescent dyes, and digitizing computers have improved contrast in light microscopy
    • Prokaryotic microorganisms include Bacteria and Archaea, while Eukaryotic microorganisms include Fungi, Protozoa, and Algae
    • Eukaryotic cells have a nucleus, cell organelles, and nucleic acid bounded by a nuclear membrane, while prokaryotic cells do not have a nucleus, have a cell wall, and nucleic acid not bounded by a nuclear membrane
    • Eukaryotic cell division occurs by mitosis and meiosis, while prokaryotic cell division occurs by binary fission
    • Eukaryotic cells have a fluid phospholipid bilayer containing sterols in the cytoplasmic membrane, while prokaryotic cells also have a fluid phospholipid bilayer in the cytoplasmic membrane

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    Test your knowledge on amino acids and protein biochemistry with this quiz. Learn about amino acid structure, classification based on side chain, protein synthesis, and protein structure. Perfect for students studying human nutrition or biochemistry.

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