Amended NIH Guidelines on Recombinant and Synthetic Nucleic Acid Molecules

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Which experiments involving Saccharomyces host-vector systems are exempt from the NIH Guidelines?

Experiments listed in Appendix C-III-A

What is the condition for using asporogenic Bacillus subtilis or Bacillus licheniformis strains in host-vector systems?

The strains should not revert to a spore former with a frequency greater than 10^-7

Under what condition are recombinant DNA molecules derived from extrachromosomal elements of Gram Positive Organisms exempt from the NIH Guidelines?

When propagated and maintained in organisms listed below

Which organisms are exempt from the NIH Guidelines when it comes to extrachromosomal elements?

Bacillus thuringiensis

What type of experiments involving transgenic rodents are exempt from the NIH Guidelines?

Experiments requiring BSL1 containment

Learn about the definition of recombinant and synthetic DNA molecules according to the amended NIH Guidelines, including criteria for molecules that can replicate in living cells and those synthesized or amplified by chemical or other means.

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