Allosteric Regulation and Respiration Basics

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Questions and Answers

Which of the following is NOT a characteristic of allosteric regulation of enzymes?

  • Exhibits sigmoidal kinetics
  • Leads to a linear relationship between substrate concentration and enzyme activity (correct)
  • Involves a regulatory subunit that is separate from the catalytic subunit
  • Allows for positive feedback regulation

In the case of uncompetitive inhibition of an enzyme, the inhibitor:

  • Binds to the enzyme-substrate complex, decreasing the Vmax (correct)
  • Binds to the enzyme, decreasing the Km
  • Binds to the substrate, preventing it from binding to the enzyme
  • Binds to the enzyme, decreasing the Vmax

Which type of enzyme inhibition results in a decrease in the Km of the enzyme?

  • Noncompetitive inhibition
  • Uncompetitive inhibition
  • Allosteric inhibition
  • Competitive inhibition (correct)

Which of the following is a characteristic of noncompetitive inhibition of an enzyme?

<p>The inhibitor binds to the enzyme, decreasing the Vmax (B)</p> Signup and view all the answers

What is the purpose of allosteric regulation in enzymes?

<p>To allow for feedback regulation of the enzyme (A)</p> Signup and view all the answers

Which of the following is a consequence of positive allosteric regulation of an enzyme?

<p>Sigmoidal kinetics (D)</p> Signup and view all the answers

What is the primary factor that regulates the activity of the enzyme phosphofructokinase (PFK) in the glycolysis pathway?

<p>The levels of ADP and AMP in the cell (D)</p> Signup and view all the answers

How does the binding of ATP to phosphofructokinase (PFK) affect the enzyme's activity?

<p>ATP binds to PFK in an allosteric manner, decreasing its activity (C)</p> Signup and view all the answers

Which of the following statements about the effects of citrate on phosphofructokinase (PFK) activity is correct?

<p>Citrate binds to PFK in an allosteric manner, decreasing its activity (A)</p> Signup and view all the answers

Which type of enzyme inhibition is exhibited by the binding of ADP and AMP to phosphofructokinase (PFK)?

<p>Allosteric inhibition (C)</p> Signup and view all the answers

How does the binding of citrate to phosphofructokinase (PFK) affect the enzyme's activity?

<p>Citrate binds to PFK in an allosteric manner, decreasing its activity (D)</p> Signup and view all the answers

Which of the following statements about the regulation of phosphofructokinase (PFK) activity is correct?

<p>PFK is inhibited by high levels of ADP and AMP, indicating the cell has sufficient energy (D)</p> Signup and view all the answers

Which of the following statements about competitive inhibitors is correct?

<p>Competitive inhibitors increase the Michaelis constant (Km) of the enzyme reaction. (D)</p> Signup and view all the answers

What is the effect of a non-competitive inhibitor on an enzyme reaction?

<p>It decreases the maximum velocity (Vmax) but does not affect the Michaelis constant (Km). (C)</p> Signup and view all the answers

Which of the following statements about uncompetitive inhibitors is true?

<p>Uncompetitive inhibitors bind to the enzyme-substrate complex, preventing product formation. (B)</p> Signup and view all the answers

Which of the following factors can affect enzyme activity?

<p>All of the above (D)</p> Signup and view all the answers

What is the difference between allosteric regulation and inhibition in enzymes?

<p>Allosteric regulation involves binding of a molecule to a regulatory site, causing a conformational change, while inhibition involves direct blocking of the active site. (C)</p> Signup and view all the answers

What is the relationship between the Michaelis constant (Km) and substrate affinity?

<p>As Km increases, substrate affinity decreases. (D)</p> Signup and view all the answers

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