Allelic Screening for β-Globin Mutation
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Questions and Answers

Which restriction enzyme site is removed due to the β-globin mutation?

  • MstII (correct)
  • BamHI
  • HindIII
  • EcoRI

    • What does it mean if the gel electrophoresis shows only one band?

  • The genotype is normal (correct)
  • The DNA cannot be digested
  • The mutation is homozygous
  • The genotype is a carrier

    • If the gel electrophoresis shows two bands, what is the genotype of the sample?

  • Carrier (correct)
  • Homozygous normal
  • Cannot be determined
  • Homozygous mutant

    • Which direction does electrophoresis start?

    <p>Negative to positive</p> Signup and view all the answers

    What does it mean if the mutant band appears ahead of the normal band on electrophoresis?

    <p>The sample is homozygous mutant</p> Signup and view all the answers

    Why is the mutant band the slowest to move on electrophoresis?

    <p>It has a larger size</p> Signup and view all the answers

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