6 Questions
Which restriction enzyme site is removed due to the β-globin mutation?
MstII
What does it mean if the gel electrophoresis shows only one band?
The genotype is normal
If the gel electrophoresis shows two bands, what is the genotype of the sample?
Carrier
Which direction does electrophoresis start?
Negative to positive
What does it mean if the mutant band appears ahead of the normal band on electrophoresis?
The sample is homozygous mutant
Why is the mutant band the slowest to move on electrophoresis?
It has a larger size
Test your knowledge on using RE digests in allelic screening with this quiz focused on detecting the β-globin mutation. Learn how to identify the presence of the A to T mutation in codon 6 through electrophoresis and the removal of the MstII restriction site. Discover how to determine carrier status based on the number of bands seen on the gel electrophoresis. Sharpen your skills in molecular biology and genetics with this informative quiz.
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