Air & Media Sterilization
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Questions and Answers

What are the methods to achieve avoidance of contamination in fermentation?

Using a pure inoculum, sterilizing the medium and fermenter vessel, maintaining aseptic conditions, and putting in place detailed operating procedures for sterilization and aseptic maintenance.

What is the target level of contamination expressed as a fraction of a cell in batch heat sterilization of liquids?

Nf 10^3

How can thermal death kinetics be represented in batch sterilization?

-dN/dt = kd N

What does the term 'D' represent in relation to sterilization time?

<p>D represents the sterilization time required to reduce the original number of viable cells by one-tenth</p> Signup and view all the answers

What factor increases the reaction rate in batch heat sterilization due to an increase in temperature?

<p>The reaction constant, kd (Death Constant)</p> Signup and view all the answers

What is the definition of sterilization?

<p>The process of destruction or elimination of all pathogenic microorganisms and organisms capable of giving rise to infection such as all spores, bacteria, fungi, etc.</p> Signup and view all the answers

Name two methods by which sterilization can be achieved.

<p>Physical, chemical, and other effective methods</p> Signup and view all the answers

Why is sterilization important in the pharmaceutical industry?

<p>It prevents the contamination of instruments and areas in the pharmaceutical industry.</p> Signup and view all the answers

How does sterilization contribute to preventing the transmission of diseases?

<p>It prevents the transmission of certain pathogenic disease-causing microorganisms into the body.</p> Signup and view all the answers

In what industries is the sterilization process commonly used?

<p>Food industries such as canning, and high-pressure methods</p> Signup and view all the answers

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