African Swine Fever Diagnosis and Necropsy

Questions and Answers

What is the purpose of the Certificate of Laboratory Compliance?

To ensure the animal is free from certain diseases (correct)

To identify the breed of the animal

To monitor the welfare of the animal

To track the movement of genetic materials

How often should ASF negative laboratory tests be conducted?

Every three months

Only once

Every six months (correct)

Every year

What type of zones can genetic materials be moved to?

Only YELLOW and LIGHT GREEN zones

Only RED and PINK zones

All zones including DARK GREEN, RED, PINK, YELLOW, and LIGHT GREEN (correct)

All zones except DARK GREEN

What is required for the source farm of semen, embryo, or ova?

Animal Welfare Registration Certificate or BAI Accredited Swine Breeder Farm Certificate Signup and view all the answers

What is included in the Veterinary Health Certificate?

Ear tag number, breed, sex, and age Signup and view all the answers

What is required to acquire an LSP?

All documentary requirements as written in DA AO 5 Series of 2019 Signup and view all the answers

Which laboratory can conduct ASF negative laboratory tests?

Any BAI-Accredited ASF Laboratory, including ADDRL and RADDL Signup and view all the answers

Why is surveillance of donor animals necessary?

To prevent the spread of disease Signup and view all the answers

What type of samples are used for ASF negative laboratory tests?

Epidemiologically feasible samples Signup and view all the answers

What is the purpose of the ASF negative laboratory test?

To prevent the spread of African Swine Fever Signup and view all the answers

Study Notes

Necropsy and Laboratory Diagnosis

Necropsy of carcasses is considered when the procedure would not pose a threat to spreading the disease further, and it must be done in a bio-secure way.
Laboratory diagnosis:
ASFV has been confirmed from swine species through detection of viral genome by Real-Time Polymerase Chain Reaction (RT-PCR).
PCR is considered the "gold standard" test for early detection of the disease due to its superior sensitivity, specificity, robustness, and high-throughput application.
Isothermal assays (e.g., convective PCR and Insulated isothermal PCR) could be a cheaper diagnostic alternative to PCR, useful in field conditions.
Serological test involving antibody detection (ELISA) is relevant for detecting surviving animals and defining the epidemiological characteristics of the epidemics.

Movement of Live Pigs and Genetic Materials

Shipment of live pigs from RED (Infected) Zones to other RED (Infected) Zones is allowed, provided required documents are complied with.
Documentary requirements for acquiring Local Shipping Permits (LSPs) include:
Animal Welfare Registration Certificate
Veterinary Health Certificate
Livestock, poultry products, and by-products Transport Carrier Registration
Livestock, poultry products, and by-products Handlers License
Certificate of Free Status for ASF (CFS-ASF)
Evidence of surveillance, including ASF negative laboratory tests from ADDRL, RADDL, or any BAI-Accredited ASF Laboratory.
Movement of live pigs for breeding purposes within RED (Infected) Zones to other RED (Infected) and PINK (Buffer) Zones is allowed, provided required documents are complied with.
Movement of genetic materials (e.g., semen, embryo, ova) from RED (Infected) Zones to other RED (Infected) Zones, YELLOW (Surveillance) Zones, PINK (Buffer) Zones, LIGHT GREEN (Protected) Zones, and DARK GREEN (Free) Zones is allowed, provided required documents are complied with.

Description

This quiz covers the procedures for diagnosing African Swine Fever, including laboratory criteria and biosecure necropsy methods. Learn about the importance of RT-PCR in detecting the viral genome and understanding the disease.