Advantages of Rotary Microtome
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Questions and Answers

Match the advantages of microtomes with their descriptions:

Heavier than rocking microtome = Increases stability during cutting No vibration while cutting = Ensures precision in sectioning Cutting angle of the knife is adjustable = Allows for flexibility in cutting technique Can be used for cutting hard tissues = Enables dissection of robust specimens

Match the features of microtomes with their benefits:

Movable knife holder = Improves ease of use Ability to cut accurate sections at 3mm = Facilitates detailed analysis Electrically driven microtome = Suitable for creating ribbon sections Used with resin embedded tissues = Enhances compatibility with various materials

Match the types of microtomes with their applications:

Manual microtome = Offers user control for precise cuts Electrically driven rotary microtome = Ideal for high-speed and consistent sectioning Cryostat microtome = Used for frozen sectioning Rocking microtome = Less stable than rotary alternatives

Match the benefits of using a microtome with their features:

<p>Can produce ribbons for serial reactions = Supports detailed tissue analysis Cuts good accurate sections = Provides high-quality results Copious with very hard tissues = Versatile for challenging specimens Employed effectively with resin embedded tissues = Optimizes processing of various samples</p> Signup and view all the answers

Match the characteristics of microtomes with their specific advantages:

<p>Adjustable cutting angle = Enhances adaptability in cutting No vibration results in cleaner cuts = Minimizes the risk of sample damage Heavy design = Contributes to steadiness during operation Can handle a variety of tissue types = Broadens applications in histology</p> Signup and view all the answers

Match the disadvantages with their descriptions:

<p>Limited block size = The size of the block that can be cut is limited. Vibration during cutting = It is a righter microtome, so it vibrates while cutting. Fixed cutting angle = The cutting angle of the knife cannot be adjusted. Curved sections = The sections cut curved when the block moves through an arc.</p> Signup and view all the answers

Match the description with its disadvantage:

<p>Limited cutting capability = No serial section is possible. Vibrations affect precision = It is a righter microtome, so it vibrates while cutting. Inflexible slicing = The cutting angle of the knife cannot be adjusted. Deformed samples = The sections cut curved when the block moves through an arc.</p> Signup and view all the answers

Match the disadvantage with its effect:

<p>Limited block size = Restricts the size of samples. Vibration = May compromise the uniformity of cuts. Fixed angle = Limits versatility during slicing. Curved sections = Results in distorted preparations.</p> Signup and view all the answers

Match the disadvantage with its characteristic:

<p>Limited block size = Affects sample processing capacity. Vibrations = Complicates accurate cutting. Fixed cutting angle = Does not allow adjustment. No serial section = Limits the ability to create multiple sections.</p> Signup and view all the answers

Match the disadvantage with the issue it causes:

<p>Size limitation = Can restrict research options. Vibrating microtome = Results in inconsistent section thickness. Knife angle rigidity = Prevents optimal slicing. Curved samples = Leads to challenges in analysis.</p> Signup and view all the answers

Match the following mathematical expressions with their correct representations:

<p>$ rac{1}{3 n}$ = Represents a fraction of 'n' $n imes m$ = Represents the product of 'n' and 'm' $ rac{1}{2} imes rac{1}{2}$ = Calculates to $ rac{1}{4}$ $ rac{1}{n} + rac{1}{m}$ = Sum of the inverses of 'n' and 'm'</p> Signup and view all the answers

Match the following components of biological structures with their functions:

<p>RNA = Transports genetic information DNA = Stores genetic instructions Protein = Performs cellular functions mRNA = Conveys information from DNA</p> Signup and view all the answers

Match the mathematical identity with its correct expression:

<p>$ rac{1}{2}(n + m)$ = Represents half the sum of 'n' and 'm' $ rac{m + n}{mn}$ = Results from adding the inverses $ rac{1}{2} imes rac{1}{2}$ = Equals to $ rac{1}{4}$ $ rac{1}{n}$ = The inverse of 'n'</p> Signup and view all the answers

Match the sections of the passage with their content:

<p>RNA = Involved in protein synthesis DNA = Holds the instructions for life Protein = Act as building blocks of the body Function = Describes the role of genetic material</p> Signup and view all the answers

Match the following mathematical operations with their results:

<p>$ rac{1}{2} imes rac{1}{2}$ = $ rac{1}{4}$ $n imes m$ = $nm$ $ rac{1}{n} + rac{1}{m}$ = $ rac{m+n}{mn}$ $ rac{1}{3 n}$ = $ rac{n}{3}$</p> Signup and view all the answers

Match the following microtome types with their descriptions:

<p>Rotary microtome = Advances an object for a predetermined distance then slides the object to the cutting tool Rocking microtome = Moves the tissue sample back and forth across the cutting blade Rotary rocking microtome = Combines the functionality of a rotary microtome and a rocking microtome Base sledge microtome = Holds the tissue sample on a sliding platform that moves across the cutting blade Sliding microtome = Advances the tissue sample and slides it across the cutting blade</p> Signup and view all the answers

Match the following microtome types with their primary purpose:

<p>Rotary microtome = General-purpose sectioning Rocking microtome = Sectioning large, soft tissue samples Rotary rocking microtome = Sectioning a variety of tissue types Base sledge microtome = Sectioning large, hard tissue samples Sliding microtome = Sectioning a variety of tissue types</p> Signup and view all the answers

Match the following microtome types with their distinguishing feature:

<p>Rotary microtome = Rotational motion to advance the tissue sample Rocking microtome = Rocking motion to move the tissue sample across the cutting blade Rotary rocking microtome = Combines rotational and rocking motions Base sledge microtome = Tissue sample is held on a sliding platform Sliding microtome = Tissue sample is advanced and slid across the cutting blade</p> Signup and view all the answers

Match the following microtome types with the tissue samples they are best suited for:

<p>Rotary microtome = General-purpose, medium-sized tissue samples Rocking microtome = Large, soft tissue samples Rotary rocking microtome = A variety of tissue types Base sledge microtome = Large, hard tissue samples Sliding microtome = A variety of tissue types</p> Signup and view all the answers

Study Notes

Advantages of Rotary Microtome

  • Heavier than rocking microtome, which allows for no vibration while cutting
  • Suitable for cutting hard tissues
  • Cutting angle of the knife is adjustable
  • Knife holder is movable, giving flexibility in cutting
  • Electrically driven microtome option for producing ribbons for serial reactions
  • Best used with resin embedded tissues
  • Can be used in cryostats

Disadvantages of Rotary Microtome

  • Limited size of block that can be cut
  • Vibration occurs while cutting
  • Cutting angle of the knife cannot be adjusted
  • Sections cut are curved when block moves through an arc
  • No serial section possible

Image Analysis

  • The passage is divided into sections on structure and function of human body and brain, RNA, DNA, Protein
  • Each section contains mathematical expressions and formulas
  • The section on Protein is highlighted with a yellow marker
  • Mathematical expressions include fraction operations, algebra, and integration

Microtomy

  • Microtomy is the process of sectioning and attaching tissues to a surface for microscopy examination
  • Basic instrument used is a microtome with a clamped cutting tool
  • Microtome is used to cut thin sections of tissue
  • Microtome machines advance an object for a predetermined distance then slide it to the cutting tool

Types of Microtome

  • Rotary microtome
  • Rocking microtome
  • Rotary rocking microtome
  • Base sledge microtome
  • Sliding microtome
  • Freezing microtome

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Description

This quiz covers the benefits of using a rotary microtome in cutting hard tissues, including its adjustable cutting angle and ability to produce accurate sections.

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