Podcast
Questions and Answers
What type of bacilli are observed in the microscopy findings?
What type of bacilli are observed in the microscopy findings?
- Gram-negative filamentous bacilli
- Gram-negative cocci
- Gram-positive branching filamentous bacilli (correct)
- Gram-positive cocci
Which culture medium is used for the growth of the specimen?
Which culture medium is used for the growth of the specimen?
- Thiosulphate broth (correct)
- Sabouraud agar
- Nutrient agar
- Blood agar
What additional technique is used for the identification of the organism?
What additional technique is used for the identification of the organism?
- Specificity identifiers and biochemical reactions (correct)
- Direct fluorescence assay
- PCR analysis
- Microscopic morphology only
What unique morphological features are noted in the microscopy observations?
What unique morphological features are noted in the microscopy observations?
Which of the following is the identified organism based on the culture and identification methods?
Which of the following is the identified organism based on the culture and identification methods?
Flashcards
Gram Staining
Gram Staining
A type of microscopic technique that stains bacteria based on their cell wall composition, revealing their shape and appearance.
Gram-positive branching filamentous bacilli
Gram-positive branching filamentous bacilli
Microscopic observation of Actinomyces israelii reveals filamentous, branching bacteria with club-shaped ends stained a distinct shade, often found in abscesses.
Culturing
Culturing
A type of laboratory test that uses a specific medium to grow bacteria, allowing scientists to observe their growth characteristics and identify them.
Actinomycetes
Actinomycetes
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MALDI-TOF
MALDI-TOF
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Study Notes
Actinomycoses - A. israelii Lab
- Specimen collection based on site of pus, lavage, or discharge
- Specimen preparation includes washes and/or filtrations
- Direct microscopy is conducted using Gram staining
- Brown-Brean modification is applied for Gram-positive branching filamentous bacilli
- Cells appear filamentous, with bacilli arranged peripherally showing hyaline and club-shaped ends
- Stains are used for histopathology, including H&E and Gomori stain
- Other stains include eosinophilic and basophilic
- Inflammatory cells like neutrophils are included in the specimen
- Inflammatory cells, like macrophages, are present
- Culture can be performed on thiosulfate-cyclohexamide broth and BHI agar
- Culture identification uses biochemical tests and a molecular approach
- PCR and MALDI-TOF are used for identification
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Description
This quiz covers the laboratory techniques involved in identifying A. israelii in actinomycoses. It discusses specimen collection, preparation, microscopy, and various staining methods used for histopathology. Additionally, it includes culture techniques and identification methods using biochemical tests and molecular approaches.
