Podcast
Questions and Answers
What type of bacilli are observed in the microscopy findings?
What type of bacilli are observed in the microscopy findings?
Which culture medium is used for the growth of the specimen?
Which culture medium is used for the growth of the specimen?
What additional technique is used for the identification of the organism?
What additional technique is used for the identification of the organism?
What unique morphological features are noted in the microscopy observations?
What unique morphological features are noted in the microscopy observations?
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Which of the following is the identified organism based on the culture and identification methods?
Which of the following is the identified organism based on the culture and identification methods?
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Study Notes
Actinomycoses - A. israelii Lab
- Specimen collection based on site of pus, lavage, or discharge
- Specimen preparation includes washes and/or filtrations
- Direct microscopy is conducted using Gram staining
- Brown-Brean modification is applied for Gram-positive branching filamentous bacilli
- Cells appear filamentous, with bacilli arranged peripherally showing hyaline and club-shaped ends
- Stains are used for histopathology, including H&E and Gomori stain
- Other stains include eosinophilic and basophilic
- Inflammatory cells like neutrophils are included in the specimen
- Inflammatory cells, like macrophages, are present
- Culture can be performed on thiosulfate-cyclohexamide broth and BHI agar
- Culture identification uses biochemical tests and a molecular approach
- PCR and MALDI-TOF are used for identification
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Description
This quiz covers the laboratory techniques involved in identifying A. israelii in actinomycoses. It discusses specimen collection, preparation, microscopy, and various staining methods used for histopathology. Additionally, it includes culture techniques and identification methods using biochemical tests and molecular approaches.
