DNA and RNA Synthesis

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21 Questions

Which Topoisomerase requires energy?

Topoisomerase II

RNA synthesis is known as

Transcription

The purpose of single-strand DNA binding proteins like replication protein A is to

keep single strand template open

What polymerase replaces DNA polymerase alpha and replicates the leading strand?

Polymerase Epsilon

What polymerase replaces DNA polymerase alpha and replicates the lagging strand?

Polymerase Delta

Errors that escape proofreading are corrected by

Mismatch repair enzymes

Base Excision Repair removes ___

Abnormal bases

Each time a chromosome divides its telomere gets shorter. Short telomeres causes cellular _____

senescence

What solves the end replication problem

Telomerase

The promoter's function is the same in prokaryotes as it is in eukaryotes. That is it defines the Transcription Start Site, defines which DNA strand is the template, and the direction of transcription.

True

RNA Polymerase terminates RNA synthesis when the newly made RNA folds into a

hairpin

All three RNA polymerases (I,II,III) are sensitive and can be inhibited by

Actinomycin D

Rifampin, a first-line antibiotic against TB, is able to bind eukaryotic RNA polymerases.

False

The purpose of the Sigma factor is to help bacterial RNA polymerase recognize the promoter

True

All RNAs require post-transcriptional processing

True

After RNA pol I transcribes, RNases cut the precursor into 3 rRNAs. These are then exported to the cytoplasm. Why?

Because ribosomes are in the cytoplasm

Nucleotidyl transferase adds CCA to

3' end

Only RNA polymerase ___ transcripts are capped

II

Abnormal hemoglobin in severe β(beta)- thalassemia caused by

splice site mutation

splicing removes ____

introns

A 7-methylguanosine-triphosphate cap protects the

5' end of eukaryotic mRNAs

Lecture 9

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