AP-HIS-503 Insitu Hybridization Detection Procedure
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Questions and Answers

If ______ is staining in the negative control, it may indicate non-specific cross reactivity to cells or cellular components.

there

What is the purpose of a standard operating procedure (SOP) for in situ hybridization (ISH)?

The purpose of a standard operating procedure (SOP) for in situ hybridization (ISH) is to explain the detection procedure for this technique.

True or false: In situ hybridization uses labeled probes to detect specific DNA or RNA target sequences in fixed or frozen tissue sections.

True

What is the purpose of this SOP?

<p>To explain the detection procedure for in situ hybridization</p> Signup and view all the answers

The most common indirect technique for ISH uses an antibody directed against an enzyme linked with a ______ system.

<p>corresponding substrate-chromogen</p> Signup and view all the answers

What is the most common indirect technique used to visualize complementary nucleic acid sequences?

<p>An antibody directed against an enzyme linked with a corresponding substrate-chromogen system</p> Signup and view all the answers

What is the indirect detection method used for in situ hybridization?

<p>The indirect detection method used for in situ hybridization is an antibody directed against an enzyme linked with a corresponding substrate-chromogen system. This combination results in a colored precipitate at the site of specific antibody binding.</p> Signup and view all the answers

True or false: The most common indirect technique used for in situ hybridization uses an antibody linked to a substrate-chromogen system.

<p>True</p> Signup and view all the answers

What is the quality control procedure for patient specimens?

<p>Examine them last</p> Signup and view all the answers

What is the quality control procedure for staining controls?

<p>The quality control procedure for staining controls is to examine them first to ascertain that all reagents are functioning properly. If there is staining in the negative control, it may indicate non-specific cross reactivity to cells or cellular components.</p> Signup and view all the answers

True or false: The ISH Iview Blue Plus Detection Kit uses a direct method to visualize complementary nucleic acid sequences.

<p>False</p> Signup and view all the answers

The ISH Iview Blue Plus Detection Kit uses an indirect method to visualize complementary nucleic acid sequences by depositing a ______ precipitate.

<p>blue</p> Signup and view all the answers

The quality control procedure for patient specimens is to examine them ______.

<p>last</p> Signup and view all the answers

What is the Insitu Hybridization Detection Procedure (HIS)?

<p>The Insitu Hybridization Detection Procedure (HIS) is a test that uses DNA or RNA probes to identify specific sequences in cells or tissue. The test is performed by heating the tissue and probe solution to denature nucleic acids, then cooling the reaction to allow the labeled nucleic acid probe to hybridize to its complementary nucleic acid sequence in the tissue.</p> Signup and view all the answers

What is the Insitu Hybridization Detection Procedure (HIS)?

<p>A test that uses DNA or RNA probes to identify specific sequences in cells or tissue</p> Signup and view all the answers

True or false: Positive or negative controls should be run concurrently with the probe slides in order to ensure accurate results.

<p>True</p> Signup and view all the answers

What is the form used for in situ hybridization?

<p>A list of key facts about the stained material and a summary of the quality control run</p> Signup and view all the answers

True or false: The ISH iVIEW Blue Plus Detection Kit can identify positive and negative results for specific nucleic acid sequences.

<p>True</p> Signup and view all the answers

What is the form used for quality control for in situ hybridization studies?

<p>The form used for quality control for in situ hybridization studies is a list of key facts about the stained material and a summary of the quality control run.</p> Signup and view all the answers

The quality control run form is a ______ of the Insitu Hybridization Detection Procedure (HIS).

<p>copy</p> Signup and view all the answers

Study Notes

  • The purpose of this standard operating procedure (SOP) is to explain the detection procedure for in situ hybridization (ISH).
  • In situ hybridization (ISH) uses labeled probes to detect specific DNA or RNA target sequences in fixed or frozen tissue sections.
  • Target sequences are exposed by heating the tissue and probe solution to denature nucleic acids.
  • The reaction is then cooled allowing the labeled nucleic acid probe to hybridize to its complementary nucleic acid sequence in the tissue.
  • The hybridization of the probe to the nucleic acid sequence is visualized with an indirect detection method.
  • The most common indirect techniques use an antibody directed against an enzyme linked with a corresponding substrate-chromogen system.
  • This combination results in a colored precipitate at the site of specific antibody binding.
  • The ISH Iview Blue Plus Detection Kit uses the indirect method to visualize complementary nucleic acid sequences by depositing a blue colored precipitate.
  • The quality control procedure for staining controls is to examine them first to ascertain that all reagents are functioning properly. If there is staining in the negative control, it may indicate non-specific cross reactivity to cells or cellular components.
  • The quality control procedure for in situ hybridization studies is to run the controls concurrently with the probe slides. The morphologic interpretation and correlation of results are performed by a qualified pathologist.
  • The quality control procedure for patient specimens is to examine them last. If the positive or negative controls fail to demonstrate appropriate staining, any results with the test specimens should be considered invalid.
  • The Insitu Hybridization Detection Procedure (HIS) is a test that uses DNA or RNA probes to identify specific sequences in cells or tissue.
  • The test is performed by heating the tissue and probe solution to denature nucleic acids, then cooling the reaction to allow the labeled nucleic acid probe to hybridize to its complementary nucleic acid sequence in the tissue.
  • The probe prep kit contains various reagents and probe types that need to be mixed before use.
  • The ISH iVIEW Blue Plus Detection Kit can identify positive and negative results for specific nucleic acid sequences.
  • Certain endometrial tissues may exhibit false positive staining when stained with the ISH iVIEWBlue Plus Detection Kit.
  • The quality control run form is a consolidated document that summarizes the key facts from the text above.
  • The quality control run form was created on 22-Feb-2023 and is currently effective.
  • The quality control run form is a copy of the Insitu Hybridization Detection Procedure (HIS).
  • The quality control run form is approved and current.
  • The quality control run form is effective starting 24-Jun-2022.

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