Lab 4: Red Blood Cell Count PDF

. Lab 4 physiology.MS.c Zainab S.J Red Blood Cell Count Red blood cell, also called erythrocyte , cellular component of blood , millions of which in the circulation of vertebrates give the blood its characteristic color and carry...

. Lab 4 physiology.MS.c Zainab S.J Red Blood Cell Count Red blood cell, also called erythrocyte , cellular component of blood , millions of which in the circulation of vertebrates give the blood its characteristic color and carry oxygen from the lungs to the tissues. The mature human red blood cell is small, round, and biconcave; it appears dumbbell - shaped in profile. The cell is flexible and assumes a bell shape as it passes through extremely small blood vessels. It is covered with a membrane composed of lipids and proteins, lacks a nucleus, and contains hemoglobin - a red , iron - rich protein that binds oxygen. The function of the red cell and its hemoglobin is to carry oxygen from the lungs to all the body tissues and to carry carbon dioxide, a waste product of metabolism, to the lungs, where it is excreted. Red blood cell count ( RBC ) signifies the number of red blood cells in a volume of blood. Normal range varies slightly between laboratories but is generally from 4.2 to 5.9 million cells/cmm. This can also be referred to as the erythrocyte count and can be expressed in international units as 4.2 to 5.9 x 1000000 cells per liter. They are smaller than white blood cells, but larger than platelets. HAEMOCYTOMETER: This is an instrument used for counting the cells in blood or fluid It consisted of a special instrument called counting chamber, cover glass, pipette for diluting the blood, rubber tube with plastic mouth piece for drawing blood or. fluid in pipette. Normal values -1 Male: 4.5 to 6.0 x1000,000 cell / cu men 2- Female: 4.0 to 4.5 x1000,000 Higher Values: lung and heart diseases Lower Values: anemia Requirement 1 - Microscope 2 - Neubauer Chamber 3 - RAC pipette 4 - RBC dilate fluid is prepared as: a) Sodium citrate: 3.0g b) formalin: 1.0 ml e) D.W to 100 ml this solution is stable at room temp (25C - 5) for at least one year Procedure: 1 - fill the red cell pipette exactly up to 0.5 mark by holding the pipette almost horizontally. The pipette must be clean and dry. 2- now draw the diluteion fluid up to 101 ( dilution 1 in 200 ) gently rotate to obtain good mixing. 3 - the cover slip is placed over the nurbaurschamber , so as to cover both the ruled platforms evenly. 4 - now load the simple. This is done in three steps : a.mix the contents of pipette for 3 minutes b.expel 6 drops from the pipette to remove the fluid in the stun , which has not been mixed with blood. c - after the load the simple wait 2 min for setting of the cell and then count. 5 - to obtain accuracy count at least 400 to 600 cells must be counted. R.B.C Calculate =dilution* volume * number of count ( N ) = 200 * 50 * N =10,000 * No. cells / cumm.