Figure 5.10: Effect of Substrate Concentration on Reaction Velocity PDF
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University of Al-Qadisiyah
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This document illustrates the concept of enzyme kinetics with reaction velocity and substrate concentration, including enzyme kinetics' parameter like Vmax and Michaelis-Menten constant.
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## Figure 5.10: Effect of Substrate Concentration on Reaction Velocity for an Enzyme-Catalyzed Reaction. * V<sub>max</sub> = maximal velocity * K<sub>m</sub> = Michaelis constant. At low concentrations of substrate ([S] << K<sub>m</sub>), the velocity of the reaction is first order. That is, it is...
## Figure 5.10: Effect of Substrate Concentration on Reaction Velocity for an Enzyme-Catalyzed Reaction. * V<sub>max</sub> = maximal velocity * K<sub>m</sub> = Michaelis constant. At low concentrations of substrate ([S] << K<sub>m</sub>), the velocity of the reaction is first order. That is, it is proportional to substrate concentration. ## D. Lineweaver-Burk plot When vo is plotted against [S], it is not always possible to determine when Vmax has been achieved, because of the gradual upward slope of the hyperbolic curve at high substrate concentrations. However, if vo is plotted versus 1/[S], a straight line is obtained (Figure 5.11). This plot, the Lineweaver-Burk plot (also called a double-reciprocal plot) can be used to calculate Km and Vmax, as well as to
