Chem40 Additional Notes PDF

Summary

This document provides notes on color reactions of proteins and amino acids, and includes experiments and results for analysis of bile pigment and bile salts.

Full Transcript

Experiment No. 7\ Color Reactions of Proteins and Amino Acids Positive Results of different test for Proteins and Amino Acids +-----------------------------------+-----------------------------------+ | Test | Result | +=======================...

Experiment No. 7\ Color Reactions of Proteins and Amino Acids Positive Results of different test for Proteins and Amino Acids +-----------------------------------+-----------------------------------+ | Test | Result | +===================================+===================================+ | Biuret Test | | +-----------------------------------+-----------------------------------+ | Ninhydrin Reaction | ![](media/image4.png) | | | | | | Positive for Proline | | | | | | Positive for α-amino acid | +-----------------------------------+-----------------------------------+ | Xanthoproteic Test | ![](media/image2.png) | +-----------------------------------+-----------------------------------+ | Pauly Reaction | | +-----------------------------------+-----------------------------------+ | Millon-Nasse Test | ![](media/image3.png) | +-----------------------------------+-----------------------------------+ | Histidine Test | | +-----------------------------------+-----------------------------------+ | Hopkins-Cole Reaction | ![](media/image6.png) | +-----------------------------------+-----------------------------------+ | Sakaguchi Reaction | | +-----------------------------------+-----------------------------------+ | Lead-Sulphide Test | ![](media/image7.png) | +-----------------------------------+-----------------------------------+ | Isatin Test | | +-----------------------------------+-----------------------------------+ Experiment No. 8\ Analysis of Bile Pigment and Bile Salt Positive Results of different test for detection of Bile Salts Test Result -------------------------- ------------------------ Smith's Test ![](media/image9.png) Pettenkofer's Test Hay's Sulfur Powder Test ![](media/image10.png) Fouchet's Test

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