Transport in Plants Lab Report PDF

# Report Sheet No. 2: Transport in Plants ## Name: ## Block: ## Date Performed: ### I. Data/Observations: #### Table 1.1 | PLANT | Distance travelled by dye (cm) after 60 minutes | Rate of Transport (cm/min) | | --------------- | ----------------------------------------------- | -----...

# Report Sheet No. 2: Transport in Plants ## Name: ## Block: ## Date Performed: ### I. Data/Observations: #### Table 1.1 | PLANT | Distance travelled by dye (cm) after 60 minutes | Rate of Transport (cm/min) | | --------------- | ----------------------------------------------- | ---------------------------- | | Intact | | | | Decapitated | | | | Cut off roots A | | | | Cut off roots B | | | | Cut off roots + | | | | decapitated | | | #### Cross-section of stems (Five samples) ### IV. Guide Questions: 1. Was the dye solution present in the xylem regions of the 5 stems? What does this indicate? 2. Was the dye solution present in the phloem regions of the 5 stems? What does this indicate? 3. Which plant sample showed the highest rate of transport? Why? 4. Which plant sample showed the lowest rate of transport? Why? 5. Why was the cut off roots B plant left in the air to dry before immersing in the dye? How did this affect the rate of transport? ### V. Conclusion: (Discuss the rate of transport in 5 samples of plants.) ## References *Note: May use extra sheets of paper for your discussions. # Activity No. 2: Transport in Plants ## Name: ## Yr.Course/Section: ## Group No. ### Introduction: To maintain the physiological active state of individual cells and whole multicellular plants, several conditions must be kept relatively constant. One among these is favorable water balance. Insufficient water supply will lead to diminished rate of development as well as reduce the rates of all vital functions. Certainly, excessive or prolonged desiccation is lethal an actively growing plant. Thus, plants require a constant supply of water because water is vital to its growth, health and survival. The main plant structure used for the absorption of water and dissolved substances are the roots. Plant cells absorb large amounts of water through the mechanisms of osmosis, inhibition and physiological active transport. ### I. Objective: To observe the various processes of transport in plants. ### II. Materials: * Dye solution (i.e. ethylene blue or eosin) * 500ml beakers or 5 big glass transparent containers * herbaceous plants (preferably Kamantigue or Mayana planted a week earlier) * razor blade * 10 pcs bond paper * foot ruler * piece of thread * glass slide and cover slips ### III. Procedure: 1. Read the entire procedure before starting. 2. Put about 200 ml of ethylene blue solution in the beaker. The plants will be immersed into this preparation. Record observation at 0 and 60 minutes 3. Obtain the 5 herbaceous plants. Be careful not to harm the roots. Wash the plants to remove the soil. Let the plants stay immersed in water. 4. Remove the plants as follows: * **Intact plant**: immerse directly into the dye solution, without cutting anything. Record the time as soon as it is immersed. * **Decapitated plant**: using the razor blade, cut off the crown of the next plant just below the lowest leaf. Immerse this plant into the dye solution. Record the time. * **Cut off roots A**: cut off the roots of another plant while the base is submerged in water. Immediately transfer this plant into the dye solution making sure that the end of the stem is not exposed to air. Record the time. * **Cut off roots B**: cut off roots in air. Leave for 15 minutes on the table. After 15 minutes, immerse the plant into the dye solution. Tie a piece of string to distinguish it from cut off A which was cut under water. Record the time. * **Cut off + decapitated**: cut off the roots of the 5th plant under water and transfer this immediately into the dye solution. Cut off the crown as well. Record the time. 5. After 60 minutes, remove the plants from the dye solution. Cut off roots B plant should be removed 5 minutes later. Rinse in tap water to remove the dye. Spread each plant on separate sheets of bond paper. Label properly. 6. Using a sharp razor blade, carefully cut a very thin section of the base of each plant and mount this on a slide (do not add water). Examine under the low power objective. Observe the position of the dye. Distinguish between xylem and phloem tissues. 7. Cut the plants longitudinally from top to bottom. Examine the sides of the stems. With the use of a foot ruler, measure the distance traveled by the dye. Tabulate your results. (The rate of transport of the dye can be computed by dividing the distance traveled by the dye by the number of minutes the plant was immersed into the solution). Fill up table 1.1. 8. Make a sketch of the cross-section of the plant examined.