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# Image Description The image shows a square divided into four colored triangles. The colors gradually transition from light to dark shades. The top of the diagram is labeled "Sample health" and the right side is labeled "Spatial resolution". The bottom left is labeled "Signal-to-noise ratio", an...

# Image Description The image shows a square divided into four colored triangles. The colors gradually transition from light to dark shades. The top of the diagram is labeled "Sample health" and the right side is labeled "Spatial resolution". The bottom left is labeled "Signal-to-noise ratio", and the bottom right is labeled "Temporal resolution". # Summary Figure 2 presents a concept relating to live imaging. It highlights the trade-offs among four key factors: sample health, spatial resolution, signal-to-noise ratio, and temporal resolution. The diagram visualizes these factors and their inter-dependence. This concept is also often called the "pyramid of frustration" because optimizing one parameter will often require compromising another.