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BIOTECHNOLOGY SKILLS • Accurately measure liquids using micropipettes and serological pipets • Accurately measure mass using electronic balances • Demonstrate proper aseptic/sterile technique • Demonstrate proper culturing of microorganisms • Demonstrate proper use of electrophoresis equipment • Pro...

BIOTECHNOLOGY SKILLS • Accurately measure liquids using micropipettes and serological pipets • Accurately measure mass using electronic balances • Demonstrate proper aseptic/sterile technique • Demonstrate proper culturing of microorganisms • Demonstrate proper use of electrophoresis equipment • Properly measure and adjust the pH of a solution with a pH meter • Properly prepare solutions, buffers, and media • Properly perform a serial dilution • Describe the applications and proper use of a spectrophotometer • Describe the proper use of a centrifuge • Use 24-hour time correctly APPLIED MATHEMATICS IN BIOTECHNOLOGY • Use scientific notation, significant digits, and decimals correctly • Perform calculations for serial dilutions • Perform calculations using dilution ratios • Make conversions within the metric system, and use metric measurements • Solution preparation: – Solve Volume/Volume (V/V) and Weight/Volume (W/V) solution calculations – Solve Molarity solution calculations – Solve Dilution Factor calculations • Generate a graph using collected data: – Apply Beer’s Law – Generate a standard curve – Properly plot data – Interpret data LABORATORY EQUIPMENT • Identify laboratory glassware and equipment • Demonstrate proper and safe use of equipment (including, but not limited to): – Fume hoods                                                   – pH Meters – Biosafety cabinets                                         – Incubators – Microscopes                                                   – Centrifuges – Electrophoresis equipment                         – Water Baths – Spectrophotometers                                     – Stirrers/Shakers – Micropipettes & serological pipets            – Vortexers – Electronic balances                                       – Autoclaves RESEARCH & SCIENTIFIC METHOD • Discuss good experimental design, including the proper use of controls • Explain the scientific method • Analyze and interpret data, including the use of statistical analysis • Explain how to maintain a laboratory notebook • Discuss various ways of communicating scientific research, including peer-reviewed journals, and presenting posters or talks at meetings