Ribosomes: Structure, Function, and Isolation PDF

Summary

This document discusses ribosomes, their structure, their role in protein synthesis, and the process of isolation and analysis.

Full Transcript

**CELL BIOLOGY** ![](media/image2.png) **RIBOSOMES** +-----------------------------------+-----------------------------------+ | -- | | | -- | | +---------------------...

**CELL BIOLOGY** ![](media/image2.png) **RIBOSOMES** +-----------------------------------+-----------------------------------+ | -- | | | -- | | +-----------------------------------+-----------------------------------+ +-----------------------------------+-----------------------------------+ | -- | | | -- | | +-----------------------------------+-----------------------------------+ ![](media/image4.png) +-----------------------------------+-----------------------------------+ | -- | | | -- | | +-----------------------------------+-----------------------------------+ +-----------------------------------------------------------------------+ | -- -- | | | | | | -- -- | | | | -- | | -- | | | | ------------------------------------------------------------------- | | -------------------------------------------------------- | | **Micrograph of ribosomes attached to the RER and the outer nuclear | | membrane under the Transmission electron microscope** | | ------------------------------------------------------------------- | | -------------------------------------------------------- | +-----------------------------------------------------------------------+ ![](media/image6.png) +-----------------------------------+-----------------------------------+ | -- | | | -- | | +-----------------------------------+-----------------------------------+ +-----------------------------------+-----------------------------------+ | -- | | | -- | | +-----------------------------------+-----------------------------------+ ![](media/image8.png) +-----------+-----------+-----------+-----------+-----------+-----------+ | -- | | | | | | | -- | | | | | | +===========+===========+===========+===========+===========+===========+ | | | | | | | +-----------+-----------+-----------+-----------+-----------+-----------+ | | | | centrifug | at | | | | | | ation | | | +-----------+-----------+-----------+-----------+-----------+-----------+ | | | | | | | +-----------+-----------+-----------+-----------+-----------+-----------+ | | | | centrifug | | | | | | | ation: | | | +-----------+-----------+-----------+-----------+-----------+-----------+ | | | | | | | +-----------+-----------+-----------+-----------+-----------+-----------+ **Ribosomes isolation and analysis**![](media/image10.png) +-----------------------+-----------------------+-----------------------+ | -- | | | | -- | | | +=======================+=======================+=======================+ | | | | +-----------------------+-----------------------+-----------------------+ | | | | +-----------------------+-----------------------+-----------------------+ | | | | +-----------------------+-----------------------+-----------------------+ | | | | +-----------------------+-----------------------+-----------------------+ | | | | +-----------------------+-----------------------+-----------------------+ | | | | +-----------------------+-----------------------+-----------------------+ | | | | +-----------------------+-----------------------+-----------------------+ | | | | +-----------------------+-----------------------+-----------------------+ | | | | +-----------------------+-----------------------+-----------------------+ | | | | +-----------------------+-----------------------+-----------------------+ | | | | +-----------------------+-----------------------+-----------------------+ +-------+-------+-------+-------+-------+-------+-------+-------+-------+ | -- | | | | | | | | | | -- | | | | | | | | | +=======+=======+=======+=======+=======+=======+=======+=======+=======+ | | | | | | | | | | +-------+-------+-------+-------+-------+-------+-------+-------+-------+ | | | | | | | | | | +-------+-------+-------+-------+-------+-------+-------+-------+-------+ | | | | | | | | | | +-------+-------+-------+-------+-------+-------+-------+-------+-------+ | | | | | | | | | | +-------+-------+-------+-------+-------+-------+-------+-------+-------+ | | | | | | | | | | +-------+-------+-------+-------+-------+-------+-------+-------+-------+ | | | | | | | | | | +-------+-------+-------+-------+-------+-------+-------+-------+-------+ | | | | | | | | | | +-------+-------+-------+-------+-------+-------+-------+-------+-------+ | | | | | | | | | | +-------+-------+-------+-------+-------+-------+-------+-------+-------+ | | large | subun | in | eukar | (23S | rRNA | | | | | | it | | yotes | | | | | +-------+-------+-------+-------+-------+-------+-------+-------+-------+ | | | | | | | | | | +-------+-------+-------+-------+-------+-------+-------+-------+-------+ | | | | | | | | | | +-------+-------+-------+-------+-------+-------+-------+-------+-------+ ![](media/image11.png) +-----------------------------------+-----------------------------------+ | -- | | | -- | | +-----------------------------------+-----------------------------------+ +--------+--------+--------+--------+--------+--------+--------+--------+ | -- | | | | | | | | | -- | | | | | | | | +========+========+========+========+========+========+========+========+ | | | When | a | **stop | **codo | **UAA* | | | | | | | ** | n** | * | | +--------+--------+--------+--------+--------+--------+--------+--------+ | | | | | | | | | +--------+--------+--------+--------+--------+--------+--------+--------+ ![](media/image13.png) +-----------------------------------+-----------------------------------+ | -- | | | -- | | +-----------------------------------+-----------------------------------+ +-----------------------------------+-----------------------------------+ | -- | | | -- | | +-----------------------------------+-----------------------------------+ ![](media/image15.png)

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