Ribosomes: Structure, Function, and Isolation PDF

**CELL BIOLOGY**  **RIBOSOMES** +-----------------------------------+-----------------------------------+ | -- | | | -- | | +---------------------...

**CELL BIOLOGY**  **RIBOSOMES** +-----------------------------------+-----------------------------------+ | -- | | | -- | | +-----------------------------------+-----------------------------------+ +-----------------------------------+-----------------------------------+ | -- | | | -- | | +-----------------------------------+-----------------------------------+  +-----------------------------------+-----------------------------------+ | -- | | | -- | | +-----------------------------------+-----------------------------------+ +-----------------------------------------------------------------------+ | -- -- | | | | | | -- -- | | | | -- | | -- | | | | ------------------------------------------------------------------- | | -------------------------------------------------------- | | **Micrograph of ribosomes attached to the RER and the outer nuclear | | membrane under the Transmission electron microscope** | | ------------------------------------------------------------------- | | -------------------------------------------------------- | +-----------------------------------------------------------------------+  +-----------------------------------+-----------------------------------+ | -- | | | -- | | +-----------------------------------+-----------------------------------+ +-----------------------------------+-----------------------------------+ | -- | | | -- | | +-----------------------------------+-----------------------------------+  +-----------+-----------+-----------+-----------+-----------+-----------+ | -- | | | | | | | -- | | | | | | +===========+===========+===========+===========+===========+===========+ | | | | | | | +-----------+-----------+-----------+-----------+-----------+-----------+ | | | | centrifug | at | | | | | | ation | | | +-----------+-----------+-----------+-----------+-----------+-----------+ | | | | | | | +-----------+-----------+-----------+-----------+-----------+-----------+ | | | | centrifug | | | | | | | ation: | | | +-----------+-----------+-----------+-----------+-----------+-----------+ | | | | | | | +-----------+-----------+-----------+-----------+-----------+-----------+ **Ribosomes isolation and analysis** +-----------------------+-----------------------+-----------------------+ | -- | | | | -- | | | +=======================+=======================+=======================+ | | | | +-----------------------+-----------------------+-----------------------+ | | | | +-----------------------+-----------------------+-----------------------+ | | | | +-----------------------+-----------------------+-----------------------+ | | | | +-----------------------+-----------------------+-----------------------+ | | | | +-----------------------+-----------------------+-----------------------+ | | | | +-----------------------+-----------------------+-----------------------+ | | | | +-----------------------+-----------------------+-----------------------+ | | | | +-----------------------+-----------------------+-----------------------+ | | | | +-----------------------+-----------------------+-----------------------+ | | | | +-----------------------+-----------------------+-----------------------+ | | | | +-----------------------+-----------------------+-----------------------+ +-------+-------+-------+-------+-------+-------+-------+-------+-------+ | -- | | | | | | | | | | -- | | | | | | | | | +=======+=======+=======+=======+=======+=======+=======+=======+=======+ | | | | | | | | | | +-------+-------+-------+-------+-------+-------+-------+-------+-------+ | | | | | | | | | | +-------+-------+-------+-------+-------+-------+-------+-------+-------+ | | | | | | | | | | +-------+-------+-------+-------+-------+-------+-------+-------+-------+ | | | | | | | | | | +-------+-------+-------+-------+-------+-------+-------+-------+-------+ | | | | | | | | | | +-------+-------+-------+-------+-------+-------+-------+-------+-------+ | | | | | | | | | | +-------+-------+-------+-------+-------+-------+-------+-------+-------+ | | | | | | | | | | +-------+-------+-------+-------+-------+-------+-------+-------+-------+ | | | | | | | | | | +-------+-------+-------+-------+-------+-------+-------+-------+-------+ | | large | subun | in | eukar | (23S | rRNA | | | | | | it | | yotes | | | | | +-------+-------+-------+-------+-------+-------+-------+-------+-------+ | | | | | | | | | | +-------+-------+-------+-------+-------+-------+-------+-------+-------+ | | | | | | | | | | +-------+-------+-------+-------+-------+-------+-------+-------+-------+  +-----------------------------------+-----------------------------------+ | -- | | | -- | | +-----------------------------------+-----------------------------------+ +--------+--------+--------+--------+--------+--------+--------+--------+ | -- | | | | | | | | | -- | | | | | | | | +========+========+========+========+========+========+========+========+ | | | When | a | **stop | **codo | **UAA* | | | | | | | ** | n** | * | | +--------+--------+--------+--------+--------+--------+--------+--------+ | | | | | | | | | +--------+--------+--------+--------+--------+--------+--------+--------+  +-----------------------------------+-----------------------------------+ | -- | | | -- | | +-----------------------------------+-----------------------------------+ +-----------------------------------+-----------------------------------+ | -- | | | -- | | +-----------------------------------+-----------------------------------+