Microbiology Internship Exam 3 PDF

Microbiology Internship Exam 3 Study online at https://quizlet.com/_a4ac88 1. How is M. tuberculosis usually trans- aerosols (inhalation) mitted? 2. What does a positive TB skin test look Red and swollen area; Indicates like and what is its significance? the individual...

Microbiology Internship Exam 3 Study online at https://quizlet.com/_a4ac88 1. How is M. tuberculosis usually trans- aerosols (inhalation) mitted? 2. What does a positive TB skin test look Red and swollen area; Indicates like and what is its significance? the individual has been exposed to MTB; may or may not have active disease. 3. What are interferon-gamma release Detects TB infection and disease; assays? Name one advantage to us- automated method for detect- ing the assays. ing interferon-gamma produced by WBCs in response to MTB anti- gens. Advantages: 1. Single patient visit 2. Less subjective to reader bias and error 3. Results unaffected by prior BCG vaccine 4. What is the appearance of mycobacte- "Ghost cells"; stain poorly if at all ria in gram-stained smears? 5. What is the recommended specimen 3 to 5 first morning specimens; 5 to collection procedure for sputum? 10 mL minimum 6. What is the recommended specimen 3 to 5 first morning specimens; 40 collection procedure for urine? mL minimum 7. How should CSF be processed? Centrifuge and inoculate media with sediment. 8. How should sputum be processed? Decontaminate and digest before media inoculation. 9. How should urine be processed? Centrifuge. Some labs may decon- taminate. 10. 1 / 17  Microbiology Internship Exam 3 Study online at https://quizlet.com/_a4ac88 How should skin/wound specimens Two sets of media should be inocu- for M. marinum be processed? lated. Incubate one set at 30 C and one set at 37 C. 11. How should gastric aspirates be han- Neutralize with sodium bicarbon- dled if they cannot be processed with- ate in 4 hours? 12. Which mycobacteria are rapid grow- M. fortinum-chelonae complex (in- ers? How fast does a rapid grower cluding M. abscessus);