AMLM Microbiology Past Paper PDF

AMLM QUESTIONS: General Microbiology 1. What percentage of microbiology is dedicated to bacteria in the lecture material? a) 50% b) 80% c) 70% d) 90% 2. Who is known as the "Father of Microbiology"? a) Robert Koch b) Antonie van Leeuwenhoek c) Loui...

AMLM QUESTIONS: General Microbiology 1. What percentage of microbiology is dedicated to bacteria in the lecture material? a) 50% b) 80% c) 70% d) 90% 2. Who is known as the "Father of Microbiology"? a) Robert Koch b) Antonie van Leeuwenhoek c) Louis Pasteur d) John Snow 3. Which microorganism group has peptidoglycan in its cell walls? a) Viruses b) Fungi c) Bacteria d) Protozoa 4. What is the main determinant of bacterial morphology? a) Cell membrane b) Cell wall c) Nucleus d) Ribosomes 5. What type of stain is the Gram stain? a) Simple stain b) Differential stain c) Acid-fast stain d) Negative stain AMLM Biosafety 6. What is the risk group for organisms handled in BSL-II labs? a) Minimal risk b) Moderate risk c) High risk d) Extreme risk 7. What biosafety level is required for handling Ebola virus? a) BSL-II b) BSL-III c) BSL-IV d) BSL-I 8. Which personal protective equipment (PPE) order is correct for donning? a) Gown → Mask → Goggles → Gloves b) Gloves → Goggles → Mask → Gown c) Goggles → Gown → Mask → Gloves d) Gloves → Gown → Mask → Goggles 9. What is the primary purpose of a biosafety cabinet (BSC)? a) Storage b) Sterilization c) Containment of aerosols d) Transportation of materials 10. How far should objects be kept from a BSC for proper airflow? a) 1 foot b) 2 feet c) 3 feet d) 5 feet Sterilization & Disinfection 11. What is the standard autoclave setting for sterilizing media? a) 121°C, 15 psi, 15-30 min AMLM b) 100°C, 1 atm, 60 min c) 132°C, 20 psi, 20-40 min d) 160°C, 15 psi, 2 hours 12. Which method is used for heat-sensitive materials? a) Dry heat b) Autoclave c) Filtration d) Incineration 13. Which gas is used for sterilizing heat-sensitive medical equipment? a) Carbon dioxide b) Hydrogen peroxide gas plasma c) Oxygen d) Ethylene oxide 14. What is the primary chemical in household bleach? a) Sodium hydroxide b) Sodium hypochlorite c) Hydrogen peroxide d) Ethanol 15. Which sterilization method is best for glassware? a) Moist heat b) Dry heat c) Chemical sterilants d) Radiation Pathogenic Microorganisms 16. Which organism causes tuberculosis? a) Mycobacterium leprae b) Mycobacterium tuberculosis c) Bacillus anthracis d) Corynebacterium diphtheriae AMLM 17. What pathogen is associated with bioterrorism in Category A? a) Ebola virus b) Brucella spp. c) Bacillus anthracis d) Clostridium botulinum toxin 18. What is the correct nomenclature for Staphylococcus? a) Staph. aureus b) S. aureus c) Staphylococcus aureus d) staphylococcus Aureus 19. Which microorganism is a common hospital-acquired infection agent? a) Escherichia coli b) Staphylococcus aureus c) Pseudomonas aeruginosa d) All of the above 20. Which pathogen is highly resistant to physical destruction? a) Gram-positive bacteria b) Gram-negative bacteria c) Prions d) Fungi Microbial Techniques 21. Which technique is used to stain acid-fast bacteria? a) Gram staining b) Ziehl-Neelsen staining c) Crystal violet staining d) Capsule staining 22. What is the role of agar in culture media? a) Nutrient source b) Solidifying agent c) Antibiotic carrier AMLM d) Indicator dye 23. What pore size is critical for sterilizing parenteral solutions? a) 0.80 µm b) 0.45 µm c) 0.22 µm d) 1.0 µm 24. Which test uses 3% hydrogen peroxide? a) Catalase test b) Oxidase test c) Coagulase test d) Urease test 25. What color indicates a positive Gram stain for Gram-positive bacteria? a) Pink b) Red c) Blue d) Purple Taxonomy and Classification 26. What is the correct order of taxonomic ranks? a) Kingdom → Class → Order → Phylum → Genus → Species b) Domain → Phylum → Kingdom → Order → Family → Genus → Species c) Domain → Kingdom → Phylum → Class → Order → Family → Genus → Species d) Kingdom → Domain → Class → Phylum → Genus → Family → Species 27. Which component is absent in prokaryotes? a) Nucleus b) Peptidoglycan c) Ribosomes d) Plasma membrane AMLM 28. What is the composition of the bacterial cell wall? a) Chitin b) Cellulose c) Peptidoglycan d) Lipopolysaccharide 29. What term describes a strain of microorganism differentiated by antigen types? a) Genotype b) Biotype c) Serotype d) Phenotype 30. What is the method used to study variations in gene expression not caused by DNA sequence differences? a) Genotyping b) Epigenetics c) Classification d) Serotyping Parasitology 31. Which biosafety level is required for handling Cryptosporidium? a) BSL-I b) BSL-II c) BSL-III d) BSL-IV 32. Which isolation precaution is used for Giardia infections? a) Contact precautions b) Droplet precautions c) Airborne precautions d) Reverse isolation 33. Which pathogen causes severe diarrhea and is transmitted through contaminated water? a) Salmonella AMLM b) Giardia c) Brucella d) Toxoplasma 34. What is the main transmission route for Salmonella? a) Airborne b) Fecal-oral c) Vector-borne d) Skin contact 35. Which of the following is a zoonotic parasitic disease? a) Cryptosporidiosis b) Tuberculosis c) Influenza d) Measles Virology 36. Which biosafety level is required for handling the Dengue virus? a) BSL-I b) BSL-II c) BSL-III d) BSL-IV 37. Which virus is classified as a bioterrorism Category A agent? a) Hepatitis B b) Smallpox c) Influenza d) Dengue 38. What is the primary structure of prions? a) RNA b) DNA c) Proteins d) Lipids AMLM 39. What causes the disease Creutzfeldt-Jakob? a) Virus b) Prion c) Bacterium d) Fungus 40. Which virus is transmitted by respiratory droplets and causes measles? a) Rubella virus b) Rubeola virus c) Parainfluenza virus d) Influenza virus Laboratory Practices 41. What is the purpose of quality control in microbiology labs? a) Identify pathogens b) Ensure consistent test accuracy c) Reduce contamination d) Minimize workload 42. Which indicator is used to confirm autoclave performance? a) Gram stain b) Biological indicators like Bacillus stearothermophilus c) Catalase test d) Acid-fast stain 43. What PPE is essential when handling airborne pathogens? a) Surgical mask b) N95 respirator c) Gloves d) Goggles 44. Which biosafety cabinet is used for handling pathogens that generate aerosols? a) BSC Class I b) BSC Class II c) BSC Class III AMLM d) Laminar flow hood 45. Which test uses sodium hypochlorite to clean surfaces contaminated with blood? a) 1:10 dilution test b) Phenol coefficient test c) Catalase test d) Gram stain Sterilization and Disinfection (Continued) 46. Which sterilization method uses gamma radiation? a) Ethylene oxide gas b) Autoclave c) Ionizing radiation d) Hydrogen peroxide gas plasma 47. What is the correct temperature for incineration of waste? a) 121°C b) 160°C c) 870-980°C d) 500°C 48. What is the best sterilization method for heat-sensitive liquids? a) Autoclave b) Filtration c) Dry heat d) UV radiation 49. What chemical is used in the Superoxol test? a) 3% hydrogen peroxide b) 30% hydrogen peroxide c) Sodium hypochlorite d) Ethanol AMLM 50. Which of the following disinfectants is sporicidal? a) Alcohol b) Sodium hypochlorite c) Phenolics d) Detergents AMLM ANSWER KEY: 17. C 35. A 18. C 36. C 1. B 19. D 37. B 2. B 20. C 38. C 3. C 21. B 39. B 4. B 22. B 40. B 5. B 23. C 41. B 6. B 24. A 42. B 7. C 25. D 43. B 8. A 26. C 44. C 9. C 27. A 45. A 10. C 28. C 46. C 11. A 29. C 47. C 12. C 30. B 48. B 13. D 31. B 49. B 14. B 32. A 50. B 15. B 33. B 16. B 34. B