Muscular Tissue Practical (lec 2pbs2)histology PDF
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Al-Arish University
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Summary
This document appears to be a set of notes on muscular tissue, including diagrams and descriptions of different types of muscle. The topics covered in the notes include the types of muscle, structure of skeletal muscle, different staining techniques, diagrams of myofibrils and other microscopic views, satellite cells and intercalated discs, and smooth muscle.
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Types of Muscle in Human body Structure of skeletal muscle Skeletal Muscle TS H&E stain Skeletal Muscle cell (cross section) H&E stain Skeletal Muscle Cells - have polygonal cross-sections (50 to 100 µm in diameter) with nuclei at the periphery Skeletal muscle LS H&E...
Types of Muscle in Human body Structure of skeletal muscle Skeletal Muscle TS H&E stain Skeletal Muscle cell (cross section) H&E stain Skeletal Muscle Cells - have polygonal cross-sections (50 to 100 µm in diameter) with nuclei at the periphery Skeletal muscle LS H&E stain Skeletal Muscle Cells - can vary in length from a few millimeters to almost a meter Myofibril Skeletal muscle striations EM of Sarcomere Satellite cells Cardiac Muscle CS H&E stain Cardiac muscle cells - have rounded cross-sections (less than 25 µm in diameter) with a centrally located nucleus. Branched Cells - cells are joined end-to-end and are often branched Cells have a single or occasionally two centrally located nuclei Intercalated discs (Phosphotungstic Acid/Hematoxylin) specialized junctions that connect the individual cells (arrows). They appear as dark purple bands across muscle fibers. Smooth Muscle LS H&E stain In relaxed smooth muscle, the nuclei are elongated with rounded ends. When contracted, the nuclei spiral, kink, or twist. The cytoplasm is pink, non- striated and with little detail. Smooth Muscle TS H&E stain individual cells vary in diameter depending on their location. Cross-sections through the middle of cells have centrally located nuclei, usually surrounded by an unstained region.
