Molecular Diagnosis ELIZA L5 PDF
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Ibn Sina National College for Medical Studies
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Summary
This document discusses molecular diagnosis techniques, including PCR (Polymerase Chain Reaction) and ELISA (Enzyme-linked Immunosorbent Assay). It describes the general principles and methods of PCR, explaining the steps involved, providing details about reagents, and showing results. It also provides information about ELISA, outlining its procedures and protocols.
Full Transcript
- PCR is based on using the ability of DNA polymerase to synthesize new strand of DNA complementary to the offered template strand. - It is widely used in molecular biology to make several copies of a specific DNA segment. Free DNA Preparation 1- PCR primers are short chain of single-stranded...
- PCR is based on using the ability of DNA polymerase to synthesize new strand of DNA complementary to the offered template strand. - It is widely used in molecular biology to make several copies of a specific DNA segment. Free DNA Preparation 1- PCR primers are short chain of single-stranded DNA, usually around 20 nucleotides in length. Two primers are used in each PCR reaction. 2- Deoxynucleoside triphosphates (dNTPs) consist of four basic nucleotides—dATP, dCTP, dGTP, and dTTP—as building blocks of new DNA strands. 3- The function of Taq DNA polymerase in PCR reaction is to amplify the DNA for the production of multiple copies of DNA. Taq DNA polymerase is a thermostable DNA polymerase which can also work at a higher temperature. https://www.youtube.co m/watch?v=JmveVAYKylk Positive Negative https://www.youtube.com/ watch?v=vq759wKCCUQ PCR Gel Sample PCR Gel PCR Results Ladder ELIZA or ELISA Enzyme-linked immunosorbent assay (ELISA) is a method of target antigen (or antibody) capture in samples using a specific antibody (or antigen), and of target molecule detection/quantitation using an enzyme reaction with its substrate. Types of ELIZA https://www.youtube.com/wa tch?v=HOHczdglqHI ELIZA 96 wells Plate
