Microscope Lesson.pptx

❑A compound light microscope uses two lenses. ❑Light passes through a sample which creates an image. ❑The image is passed through an objective lens first, then an eyepiece before reaching your eye. ❑The two lenses work together to magnify the image so that it appears larger to your eye. ❑There ar...

❑A compound light microscope uses two lenses. ❑Light passes through a sample which creates an image. ❑The image is passed through an objective lens first, then an eyepiece before reaching your eye. ❑The two lenses work together to magnify the image so that it appears larger to your eye. ❑There are several parts to a microscope, each having a specific function and purpose. ❑Let’s look at each part of the microscope and its use. ❑Eyepiece: ❑AKA the ocular lens ❑A place to view the specimen ❑Body Tube ❑Revolving Nosepiece: ❑Rotates to change between different objective lenses ❑Objective Lenses: ❑3 or 4 difference lens choices ranging from 4X to 40X ❑Low (4X)-shortest ❑Medium (10X) ❑High (40X)longest ▪ This lens is closest to the specimen ❑Stage: ❑Holds the slide and has a window that allows light to pass through the specimen ❑Diaphragm: ❑Turns to adjust the amount of light that passes through the ❑Arm: ❑Allows user to hold the microscope securely ❑Base: ❑Supports the microscope ❑Stage Clips: ❑These clips hold a slide in place on the stage for viewing ❑Light Source: ❑Shines light upward through the specimen and to the lenses ❑Fine Adjustment Knob: ❑Fine tunes the focus ❑Used with all ❑Coarse Adjustment objective lenses Knob: ❑Moves the stage up and down ❑Used only with low and medium Objective Lens Eye Piece Total Magnification Calculating 4X 10X Magnification 10X 10X 40X 10X ❑ Always carry the microscope by the arm and the base ❑ Always start viewing a specimen on the lowest power ❑ Adjust the diaphragm to ensure light is passing through the specimen ❑ Clean the lens with lens paper only ❑ Return the objective lens to LOW power when finished ❑ Ensure that slides are removed from the microscope and the cord is wrapped around the microscope ❑ Keep the microscope clean. ❑ Keep it covered when not in use ❑ Do not use the coarse adjustment knob with the high power objective lenses. YOU COULD BREAK THE SLIDE. ❑ Don’t put specimens directly on the microscope stage. Always use a prepared slide ❑ Don’t touch the lenses with your hands or other objects. eyepiece body tube Let’s Practice! nose piece low power med. power high power stage clips diaphrag m Light arm stage coarse adj. knob fine adj. knob base