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INFECTION CONTROL PRACTICES IN THE Prosthodontics and DENTAL LABORATORY INTRODUCTION Infection control (IC) is an essential part of dentistry Potential for disease transmission in the dental lab is well documented Potential pathogens can be transported to lab via orally soiled impressions, dental...
INFECTION CONTROL PRACTICES IN THE Prosthodontics and DENTAL LABORATORY INTRODUCTION Infection control (IC) is an essential part of dentistry Potential for disease transmission in the dental lab is well documented Potential pathogens can be transported to lab via orally soiled impressions, dental prostheses/appliances Microorganisms can be transferred from contaminated impressions to dental casts – Oral bacteria can remain viable in set gypsum for up to 7 days EXPOSURE Lab personnel may be exposed via -Direct contact (through cuts and abrasions) -Aerosols created during lab procedures Inhaled or ingested Patients can be at risk due to potential crosscontamination between dental prostheses/ appliances Potential for cross-contamination from dental office to lab and back to dental office EXPOSURE Potential infection can be transferred in lab from case to case By surface contact, handpieces, burs, pumice pans, aerosolization, dust/mist, unwashed hands CROSS-CONTAMINATION • Passage of microorganisms from one person or inanimate object to another • Aseptic techniques* must be implemented to reduce occurrence *Procedures that break the chain of infection to reduce cross-contamination • Dentists and lab should establish IC protocol for incoming and outgoing cases CHAIN OF INFECTION • All links must be connected for infection to take place Pathogen (sufficient virulence & adequate numbers) Susceptible Host (i.e., one that is not immune) Entry (portal that the pathogen can enter the host) Source (allows pathogen to survive & multiply) Mode (of transmission from source to host) GOALS/ACTIONS • Strive to make dental lab as safe as possible • Minimize potential for disease transmission via • Immunizations • Barrier techniques • Aseptic techniques • IC compliance • Adhere to Standard Precautions (SP) • Establish written IC policy STANDARD PRECAUTIONS • Must be observed in the lab at all times • Are used by all lab personnel to prevent crosscontamination by dental items entering lab • All patients are treated as if they could transmit a bloodborne pathogen (BBP) disease • Examples include hepatitis B, hepatitis C, and human immunodeficiency virus (HIV) REQUIREMENTS • Lab is responsible to comply and enforce all federal, state, and local regulations that affect its operations and employees • Includes the Occupational Safety and Health Administration’s (OSHA) BBP Standard • All lab personnel • Must be included in exposure determination • Must be offered hepatitis B vaccine • Must be given annual BBP training IC POLICY • Written • Should be precise, concise, and easy to understand • Dynamic process • Reviewed annually and updated whenever necessary • Cover occupational exposure incidents • Individual must be provided with counseling, post-exposure evaluation, and follow-up BASICS OF LABORATORY IC • Need coordination between dental office and lab • Use of proper methods/materials for handling and decontaminating soiled incoming items • All contaminated incoming items should be cleaned and disinfected before being handled by lab personnel, and before being returned to the patient COMMUNICATION • Is essential between lab and dental office • To ensure proper procedures are followed • Concerning IC protocols • Clearly describe requirements for case submission • Specifically delineate responsibilities • Personnel must understand the proper steps to ensure disinfection of materials entering lab • Reason: to prevent duplication of disinfection protocol, and to prevent potential lab contamination • If uncertain on status: disinfect by prescribed methods “BARRIER” SYSTEM Is most effective, practical method for preventing cross-contamination Is a series of physical cleaning procedures to reduce organic debris and microorganisms on intraorally soiled dental items Accomplished through step-wise process of mechanical and chemical cleaning and disinfection Results in a product that can safely be handled by lab personnel without need for personal protective equipment (PPE) BARRIERS Include Handwashing with plain or antimicrobial soap (or an alcohol-based hand rub if hands are not visibly soiled) Use of PPE when there is potential for occupational exposure to BBPs Examples Gloves Mask Protective eyewear, chin length face shield Protective clothing (i.e., lab coat/jacket) Note: BBP= Blood born pathogens PPE= Personnel protective equipment GLOVES • Disposable gloves • Use when there is potential for direct hand contact with contaminated items • Should be changed and disposed of appropriately after completion of procedure • Hands should be washed before gloving and after removing gloves • Utility gloves • Should be used when cleaning/disinfecting equipment/surfaces MASK/PROTECTIVE EYEWEAR/CLOTHING • Must be used when there is potential for splashes, spray, spatter, or aerosols • Examples: when operating lathes, model trimmers, and other rotary equipment • Lab coat/jacket should be worn at all times during fabrication process • Change daily • Do not wear outside of the lab • Launder appropriately UNIT-DOSE CONCEPT • Purpose: to minimize cross-contamination • Refers to dispensing of amount of material(s) sufficient to accomplish a particular procedure, prior to patient contact • Dispose of excess material(s) at completion of procedure CHEMICAL DISINFECTANTS • Two functions • Must be an effective antimicrobial agent • Must not adversely affect dimensional accuracy or surface texture of impression materials and resulting gypsum cast • Want to reduce likelihood of ill fitting, nonfunctional prostheses CHEMICAL DISINFECTANTS • All employees must be properly trained to handle these materials in accordance with OSHA’s Hazard Communication Standard • Disinfectant must have an Environmental Protection Agency (EPA) registration number • Must have at least intermediate-level of activity • Tuberculocidal, hospitalgrade DENTAL LABORATORY • All disinfection procedures are accomplished prior to delivery to lab • Done in dental operatory or professional work area • Recommend a sign and monitor system be implemented stating “Only Biologically Clean Items Permitted” INCOMING ITEMS • Rinse under running tap water to remove blood/saliva • Disinfect as appropriate • Rinse thoroughly with tap water to remove residual disinfectant • No single disinfectant is ideal or compatible with all items • Annotate the DD Form 2322: “Disinfected with ______ for _____minutes” OUTGOING ITEMS • Clean and disinfect before delivery to patient • After disinfection: rinse and place in plastic bag with diluted mouthwash until insertion • Do not store in disinfectant before insertion • Label the plastic bag: “This case shipment has been disinfected with ______ for IMPRESSIONS • Many studies have been performed to evaluate effects of various disinfectants on different types of impression materials • Research findings have been contradictory • No single disinfectant is compatible with all impression materials • The least distortion is associated with products having the shortest contact times IMPRESSIONS • Many variables can affect impression materials • Composition and concentration of disinfectants • Exposure time and compatibility of various disinfectants with specific impression materials • Physical/chemical properties can vary in a given category of material or disinfectant • Do an in-office “test run” when using new combinations of impression materials and disinfectants • Consult dental materials’ manufacturers regarding their compatibility with disinfectants DISINFECTING IMPRESSIONS • Methods • Spraying, dipping, immersing • Exposure time should be that recommended by the manufacturer of disinfectant for tuberculocidal disinfection • Iodophors, sodium hypochlorite (1:10 concentration), chlorine dioxide, phenols, and other approved products DISINFECTING IMPRESSIONS • Polyether materials cannot be immersed in disinfectants due to potential for absorption and distortion • Immersion disinfectants can only be used once before discarding (except for glutaraldehydes) • Most reports indicate dimensional stability is not significantly affected by immersion technique Disinfection METHODS OF DISINFECTING IMPRESSIONS ⦿ 1.Spraying, Dipping & ⦿ 2. Immersing methods ⦿ Disinfection of Alginate Impressions with 0.5% sodium hypochlorite. Iodophors Immersion disinfection for prolonged periods will cause distortion due to imbibition Disinfection METHODS OF DISINFECTING IMPRESSIONS ⦿ Agar- Reversible Hydrocolloid ⦿ Found to be stable when immersed in 1:10 dilution sodium hypochlorite or 1:213 iodophor. Recommended immersion time is 10 minutes. Disinfection METHODS OF DISINFECTING IMPRESSIONS ⦿ Zinc Oxide Eugenol Immersion ⦿ 2% glutaraldehyde Iodophors or Chlorine compounds. ⦿ Adverse effect have been reported on ZOE immersed for 16 hours in diluted hypochlorite. Disinfection METHODS OF DISINFECTING IMPRESSIONS ⦿ Impression Compound Immersion ⦿ 1:10 dilution sodium hypochlorite or iodophor for specified time period. Disinfection METHODS OF DISINFECTING IMPRESSIONS ⦿ ELASTOMERIC IMPRESSION MATERIALS ⦿ Polysulphide and Addition Silicone: Glutaraldehyde, Iodophor, 0.5% sodium hypochlorite should be used Disinfection METHODS OF DISINFECTING IMPRESSIONS ⦿ Polyether: ⦿ Spraying in iodophor, 0.5% Sodium hypochlorite should be used. ⦿ Prolonged immersion causes some distortion. ⦿ Polyether shows dimensional changes on immersion in 2% glutaraldehyde Disinfection Exposure time should be that recommended by the manufacturer of disinfectant for tuberculocidal disinfection. ⦿ Iodophors, 5.52% sodium hypochlorite (1:10 concentration) with minimum contact time of 10mins , chlorine dioxide, phenols, and other approved products are all acceptable DISINFECTING IMPRESSIONS • Clean and rinse impression in dental operatory • Cleaning efficiency can be improved by gently scrubbing impression with camel’s hair brush and antimicrobial detergent • Sprinkle dental stone into impression before rinsing to aid in cleaning • Cleaning and rinsing • Reduces bioburden present • Lessens overall microbiologic challenge to DISINFECTING IMPRESSIONS • Spray, dip, or immerse impression in appropriate intermediate- or high-level disinfectant and place in sealed bag • Disinfection can be accomplished in the dental operatory or a professional work area depending on facility policy • After required contact time, rinse impression and pour-up Disinfection Spray Techninque ⦿ Rinse entire impression/tray under running tap water after removal from oral cavity ⦿ Trim excess impression material from noncritical areas – Reduces number of microorganisms and organic debris present Disinfection ⦿ Place impression in bag and liberally spray the entire impression/tray. ⦿ Seal bag to create “charged atmosphere” – Reduces exposure to vapors and liquid SPRAY TECHNIQUE • Remove from bag at end of exposure time; rinse and pour • Once stone has set, remove cast from impression • Dispose of impression material and disposable tray (if applicable) in general waste • Sterilize reusable tray (if applicable) SPRAY DISINFECTION Pros and Cons • Advantages • Uses less disinfectant • Same disinfectant can often be used to disinfect environmental surfaces • Disadvantages • Probably not as effective as immersion • Can be released into air increasing occupational exposure Dipping /Immersion Technique ⦿ Select disinfectant with short exposure time to minimize distortion and deterioration of surface quality of resulting stone cast. Disinfection ⦿ Follow same procedures as above except fully immerse or dip impression in disinfectant for recommended exposure time ⦿ Do not use water based glutaraldehyde solutions with a pH of 8 or higher for since this might affect the gypsum surface DENTAL CASTS • Very difficult to disinfect • Cast should be fully set (at least 24 hrs) before disinfection • Is preferable to disinfect impression • If casts must be disinfected: • Place casts on end to facilitate drainage • Spray with iodophor or chlorine product, then rinse • Another option • Soak casts for 30 minutes in 0.5% concentration of sodium hypochlorite and saturated calcium dihydrate solution (SDS) • SDS is produced by placing uncontaminated, set gypsum (i.e. ORALLY SOILED PROSTHESES • Scrub with brush and antimicrobial soap to remove debris and contamination • Can be accomplished in operatory or professional work area • Sterilize brush or store in approved disinfectant • Place prosthesis in sealable plastic bag or beaker filled with ultrasonic cleaning solution or calculus remover ORALLY SOILED PROSTHESES • Place in ultrasonic cleaner for required time as specified by manufacturer of ultrasonic cleaner • Place cover on ultrasonic cleaner to reduce spatter potential • Remove and rinse under running tap water, dry, and accomplish required work SUB-SURFACE DISINFECTION • Place prosthesis in sealable plastic bag containing 1:10 dilution of sodium hypochlorite or other intermediate- to highlevel disinfectant (not glutaraldehyde or phenols) • Place in ultrasonic cleaner for 10 minutes DENTAL PROSTHESES • Do not exceed manufacturer’s recommended contact time on metal components to minimize corrosion • There is little effect on chrome-cobalt alloy with short-term exposures (10 minutes) • Do not store in disinfectant before insertion • Store in diluted mouthwash until insertion WASTE • Can include disposable trays, impression materials, and contaminated packing materials (if cannot be disinfected) • Dispose of according to applicable federal, state, and local regulations • Dispose of in general waste unless defined as regulated waste • Only small amounts of regulated waste are generated in lab • Sharps should be placed in puncture-resistant container LATHE • Ways to reduce risk of injury from aerosols, spatter, and macroscopic particles • Use protective eyewear • Ensure plexiglass shield is in position • Activate vacuum LATHE • Pumice has been shown to pose a potential contamination risk • Via aerosol or direct contact • Mix pumice with • Clean water, diluted 1:10 bleach, or other appropriate disinfectant • Add tincture of green soap if desired LATHE • Change pumice daily • Machine should be cleaned and disinfected daily • No need for separate pans for new and existing prostheses if isolated properly • At a minimum clean and disinfect pumice brushes and rag wheels daily. Daily heat STERILIZATION • Heat sterilize all metal and heat-stable instruments that contact oral tissues, contaminated appliances, or potentially contaminated appliances should be heat sterilized after each use • Examples: facebow fork, metal impression trays, burs, polishing points, rag wheels, laboratory knives Impression trays ⦿ Pre-cleaning removes bio-burden and any adherent impression material. ⦿ Ultrasonic cleaning can aid in removing residual set gypsum. ⦿ Chrome-plated or aluminum trays – Clean, package & heat sterilize. Disinfection ⦿ Single-use trays – Discard after one use. ⦿ Custom acrylic trays – Can be disinfected (by spray or immersion), then rinsed (if to be used for second appointment). DISINFECTION Prosthodontic items contaminated by handling should be disinfected (by spray or immersion technique based on type of item) after each use. • Examples: alcohol torch, facebow, articulator, mixing spatula, mixing bowl, lab knife, shade/mold guide Wax Bites/Rims, Bite Registration ⦿ Immersion disinfection may cause distortion to some items – Use spray disinfection. ⦿ Heavy-body bite registration materials – Usually not susceptible to distortion and can be disinfected in same manner as an impression of the same material Disinfection CLEANING AND DISINFECTION STRATEGIES FOR BLOOD SPILLS ⦿ Strategies for decontaminating spills of blood and other body fluids differ by setting and volume of the spill. LAB EQUIPMENT • Follow manufacturer instructions for: • Maintenance • Cleaning • Disinfection • Compatibility with disinfectants ENVIRONMENTAL SURFACES • Disinfection procedures should be comparable to procedures performed in the operatory • Clean and disinfect daily or when visibly contaminated • Use EPA-registered, tuberculocidal, hospital-grade disinfectant according to manufacturer instructions • Use utility gloves • May use surface barriers to reduce the need to use disinfectants PERSONAL HYGIENE • Refrain from the following activities while in the lab where there is potential for occupational exposure: • Eating • Drinking • Smoking • Applying cosmetics or lip balm • Handling contact lenses SPECIAL CONSIDERATIONS • For porcelain restorations that are characterized intraorally • Take them directly to porcelain furnace • Sintering process sterilizes restoration • No need for separate cleaning/disinfection process • Monitor procedures closely to ensure proper cleaning/disinfection of equipment and areas that may become contaminated during the process PREVENTION IS BETTER THAN CURE”- a proverb well suited to sterilization A thorough understanding of the application of sterilization will help ensure safety from the invisible but deadly world of microbial pathogens • Hence utilization of proper sterilization, disinfectants and aseptic procedures helps us achieve the safety our profession • SUMMARY • Dental lab presents numerous challenges to IC • Lab personnel are at risk of occupational exposure to BBPs • Disease transmission from contaminated items entering the lab can be prevented SUMMARY • Best safeguards • Adherence to SP at all times • Hepatitis B vaccine • Implementation of aseptic techniques • Use of PPE, unit-dosing of materials, barriers • Use of appropriate sterilization and disinfection procedures SUMMARY • All IC activities are designed to accomplish one goal • Break the link in the chain of infection • Want to interrupt potential for person-to-person transmission of infection • Processes must be performed consistently and routinely to be effective • Requires communication and coordinated effort between lab and dental office • Redundancies must be identified and minimized