Glycolysis Lecture Notes PDF

Glycolysis Bas Teusink Glycolysis: glucose to pyruvate Gluconeogenesis: “pyruvate” to glucose AB_1137 Biochemistry 2019 2  Glycolysis: glucose to pyruvate n: o l i sm s is tio etab...

Glycolysis Bas Teusink Glycolysis: glucose to pyruvate Gluconeogenesis: “pyruvate” to glucose AB_1137 Biochemistry 2019 2  Glycolysis: glucose to pyruvate n: o l i sm s is tio etab th e n c Fu rgy m ADH : i o syn N ion for b E ne a n d nc t r ATP Fu curso Pre Fig 22.13 Fig 22.1 AB_1137 Biochemistry 2019 3 Glycolysis: counting electrons C0 + 4e- 2x (C3-, C2+, C3+ = C2+) AB_1137 Biochemistry 2019 4 Glycolysis: two parts C6 Glucose + 2 ADP + 2 NAD+ → 2 pyruvate + 2 NADH + 2 ATP bis C6 Carbon and electrons are ok, C3 but where is the phosphate? C3 Fig 22.4 AB_1137 Biochemistry 2019 5 Glycolysis: counting phosphates C6 in : 2 ATP out: 2 ADP bis C6-P2 C3-P 2 Pi in : 4 ADP in: 2 Pi out: 4 ATP C3 Fig 22.4 AB_1137 Biochemistry 2019 6 Glycolysis: better representation… AB_1137 Biochemistry 2019 7 ”pumps” of glycolysis sites of regulation Hexokinase, phosphofructokinase, pyruvate kinase AB_1137 Biochemistry 2019 8 ”pumps” of glycolysis sites of regulation Hexokinase, phosphofructokinase, pyruvate kinase AB_1137 Biochemistry 2019 9 G6P: important node Fig 22.5 Fig 22.14 AB_1137 Biochemistry 2019 10 PFK first committed (irreversible) step PFK Fig 22.5 Fig 22.14 AB_1137 Biochemistry 2019 11 Glycolysis makes ATP: ATP inhibits pump PFK PFK Fig 22.16B Fig 22.1 AB_1137 Biochemistry 2019 12 PFK regulated by energy need: AMP and ATP Adenylate kinase equilibrium: ATP + AMP ⇋ 2 ADP High AMP is sensitive signal for low energy level AB_1137 Biochemistry 2019 13  Okay, what do I need to know? Overall pathway architecture, with Glc6P and pyruvate as important nodes counting electrons & phosphates What the key regulatory steps (pumps) are, and why The logic of activation and inhibition of the key enzymes (more to come in next lecture…) AB_1137 Biochemistry 2019 14