Lecture 5 Notes - Microbiology PDF

Int. J. Environ. Res. Public Health 2010, 7, 3657-3703; doi:10.3390/ijerph7103657 OPEN ACCESS International Journal of...

Int. J. Environ. Res. Public Health 2010, 7, 3657-3703; doi:10.3390/ijerph7103657 OPEN ACCESS International Journal of Environmental Research and Public Health ISSN 1660-4601 www.mdpi.com/journal/ijerph Review Water Microbiology. Bacterial Pathogens and Water João P. S. Cabral Center for Interdisciplinary Marine and Environmental Research (C. I. I. M. A. R.), Faculty of Sciences, Oporto University, Rua do Campo Alegre, 4169-007 Oporto, Portugal; E-Mail: [email protected]; Tel.: +351-220402751; Fax: +351-220402799. Received: 19 August 2010; in revised form: 7 September 2010 / Accepted: 28 September 2010 / Published: 15 October 2010 Abstract: Water is essential to life, but many people do not have access to clean and safe drinking water and many die of waterborne bacterial infections. In this review a general characterization of the most important bacterial diseases transmitted through water— cholera, typhoid fever and bacillary dysentery—is presented, focusing on the biology and ecology of the causal agents and on the diseases‘ characteristics and their life cycles in the environment. The importance of pathogenic Escherichia coli strains and emerging pathogens in drinking water-transmitted diseases is also briefly discussed. Microbiological water analysis is mainly based on the concept of fecal indicator bacteria. The main bacteria present in human and animal feces (focusing on their behavior in their hosts and in the environment) and the most important fecal indicator bacteria are presented and discussed (focusing on the advantages and limitations of their use as markers). Important sources of bacterial fecal pollution of environmental waters are also briefly indicated. In the last topic it is discussed which indicators of fecal pollution should be used in current drinking water microbiological analysis. It was concluded that safe drinking water for all is one of the major challenges of the 21st century and that microbiological control of drinking water should be the norm everywhere. Routine basic microbiological analysis of drinking water should be carried out by assaying the presence of Escherichia coli by culture methods. Whenever financial resources are available, fecal coliform determinations should be complemented with the quantification of enterococci. More studies are needed in order to check if ammonia is reliable for a preliminary screening for emergency fecal pollution outbreaks. Financial resources should be devoted to a better understanding of the ecology and behavior of human and animal fecal bacteria in environmental waters. Int. J. Environ. Res. Public Health 2010, 7 3658 Keywords: drinking water; cholera; typhoid fever; bacillary dysentery; fecal indicator bacteria; coliforms; ammonia 1. Drinking Water as a Vehicle of Diseases Water is essential to life. An adequate, safe and accessible supply must be available to all. Improving access to safe drinking-water can result in significant benefits to health. Every effort should be made to achieve a drinking water quality as safe as possible. Many people struggle to obtain access to safe water. A clean and treated water supply to each house may be the norm in Europe and North America, but in developing countries, access to both clean water and sanitation are not the rule, and waterborne infections are common. Two and a half billion people have no access to improved sanitation, and more than 1.5 million children die each year from diarrheal diseases. According to the WHO, the mortality of water associated diseases exceeds 5 million people per year. From these, more that 50% are microbial intestinal infections, with cholera standing out in the first place. In general terms, the greatest microbial risks are associated with ingestion of water that is contaminated with human or animal feces. Wastewater discharges in fresh waters and costal seawaters are the major source of fecal microorganisms, including pathogens [1-4]. Acute microbial diarrheal diseases are a major public health problem in developing countries. People affected by diarrheal diseases are those with the lowest financial resources and poorest hygienic facilities. Children under five, primarily in Asian and African countries, are the most affected by microbial diseases transmitted through water. Microbial waterborne diseases also affect developed countries. In the USA, it has been estimated that each year 560,000 people suffer from severe waterborne diseases, and 7.1 million suffer from a mild to moderate infections, resulting in estimated 12,000 deaths a year. The most important bacterial diseases transmitted through water are listed in Table 1. Table 1. The main bacterial diseases transmitted through drinking water. Disease Causal bacterial agent Cholera Vibrio cholerae, serovarieties O1 and O139 Gastroenteritis caused by vibrios Mainly Vibrio parahaemolyticus Typhoid fever and other serious Salmonella enterica subsp. enterica serovar Paratyphi salmonellosis Salmonella enterica subsp. enterica serovar Typhi Salmonella enterica subsp. enterica serovar Typhimurium Bacillary dysentery or shigellosis Shigella dysenteriae Shigella flexneri Shigella boydii Shigella sonnei Acute diarrheas and gastroenteritis Escherichia coli, particularly serotypes such as O148, O157 and O124 Int. J. Environ. Res. Public Health 2010, 7 3659 2. Cholera 2.1. The Genus Vibrio Vibrio are small, curved-shaped Gram-negative rods, with a single polar flagellum. Vibrios are facultative anaerobes capable of both fermentative and respiratory metabolism. Sodium stimulates growth of all species and is an absolute requirement for most. Most species are oxidase-positive and reduce nitrate to nitrite. Cells of certain species (V. cholerae, V. parahaemolyticus and V. vulnificus) have pili (fimbriae), structures composed of protein TcpA. TcpA formation is co-regulated with cholera toxin expression and is a key determinant of in vivo colonization (see below) [7,8]. Several Vibrio species can infect humans (Table 2). V. cholerae is, by far, the most important of these species. V. alginolyticus has been isolated from several types of soft tissue infections. Table 2. Main species of Vibrio and their occurrence in human clinical specimens a. Occurrence in human Main species clinical specimens Intestinal Extra-intestinal Vibrio alginolyticus + ++ Vibrio cholerae O1 and O139 +++++ + Vibrio cholerae non O1 or O139 ++ ++ Aliivibrio fischeri (Vibrio fischeri) - - Vibrio fluvialis ++ - Vibrio furnissii ++ - Vibrio harveyi - + Grimontia hollisae (Vibrio hollisae) ++ - Vibrio mimicus ++ + Vibrio natriegens - - Vibrio parahaemolyticus ++++ + Vibrio vulnificus + +++ a Adapted from [7,8]. Nomenclature according to. The symbols give the relative frequency of each organism in human clinical specimens, and apply to the whole World, rather than to a particular country. V. fluvialis, Grimontia hollisae (V. hollisae), and V. mimicus can cause diarrhea or infections of the gastrointestinal tract. V. furnissii has been isolated from a few individuals with diarrhea, but there is no evidence that it can actually cause this pathology. V. parahaemolyticus is a well-documented causal agent of acute food-borne gastroenteritis, particularly in Japan and South East Asia. Cases are associated with the consumption of raw or undercooked shellfish such as oysters, shrimp, crabs, and lobster. V. vulnificus is an important cause of (often fatal) septicemia and wound infections. Other vibrios, namely Allivibrio fischeri (Vibrio fischeri) and V. natriegens, have no relation with humans [7,8]. Vibrios are primarily aquatic bacteria. Species distribution depends on sodium concentration and water temperature. Vibrios are very common in marine and estuarine environments, living free or on Int. J. Environ. Res. Public Health 2010, 7 3660 the surfaces and in the intestinal contents of marine animals. Species with a low sodium requirement are also found in freshwater habitats [7,8]. 2.2. The Species Vibrio cholerae Vibrio cholerae cells can grow at 40 °C with pH 9–10. The growth is stimulated by the presence of sodium chloride. Vibrio cholerae is a very diverse bacterial species (Table 3). It is divided in ca. 200 serovarieties, characterized by the structure of the lipopolysaccharide (LPS) (O antigens). Only serovarieties O1 and O139 are involved in ―true‖ cholera. Some other serovarieties can cause gastroenteritis, but not cholera. The distinction between Classical and El Tor biotypes is based on biochemical and virological characteristics [1,7,8,10,11]. Table 3. Subdivision of Vibrio cholerae below the species level a. Serovariety Serotype Biotype O1 Inaba Classical El Tor Ogawa Classical El Tor Hikojima O139 others a Adapted from. 2.3. Cholera 2.3.1. Characterization of the disease The incubation period for cholera is ca. 1–3 days. The disease is characterized by an acute and very intense diarrhea that can exceed one liter per hour. Cholera patients feel thirsty, have muscular pains and general weakness, and show signs of oliguria, hypovolemia, hemoconcentration, followed by anuria. Potassium in blood drops to very low levels. Patients feel lethargic. Finally, circulatory collapse and dehydration with cyanosis occurs. The severity of the disease depends on several factors: (1) personal immunity: this may be conferred by both previous infections and by vaccines; (2) inoculum: the disease only occurs after ingestion of a minimum amount of cells, ca. 108 [1,7,8,10,11]; (3) The gastric barrier: V. cholera cells likes basic media and therefore the stomach, normally very acidic, is an adverse medium for bacterial survival. Patients consuming anti-acidic medications are more susceptible to infection than healthy people; (4) blood group: for still unknown reasons, people with O-group blood are more susceptible than others [1,7,8,10,11]. In the absence of treatment, the mortality of cholera-patients is ca. 50%. It is mandatory to replace not only lost water but also lost salts, mainly potassium. In light dehydrations, water and salts can be orally-administered, but in severe conditions, rapid and intravenous-administration is obligatory. The most efficient antibiotic is currently doxicyclin. If no antibiotic is available for treatment, the Int. J. Environ. Res. Public Health 2010, 7 3661 administration of water with salts and sugar can, in many cases, save the patient and help in the recovery [1,7,8,10,11]. There are two main determinants of infection: (1) the adhesion of the bacterial cells to the intestinal mucous membrane. This depends on the presence of pili and adesins at the cell‘s surface; (2) the production of cholera toxin [1,7,8,10,11]. 2.3.2. Cholera toxin Cholera toxin is an exotoxin with a very precise action on target cells. The toxin attaches to a specific receptor (ganglioside Gl) on the cell membrane of intestinal cells and activates the enzyme adenylate cyclase. This results in a non-stop degradation of internal ATP, with release of cAMP and inorganic phosphate. The rise in the internal concentration of cAMP causes an efflux of water, sodium, potassium, chloride and carbonate ions from the cells of the mucous membrane, and this is the main cause of diarrhea. 2.3.3. Cholera pandemics and the emergence of El Tor biotype and O139 serovariety. New facts about cholera epidemiology Cholera has been a well known disease since the 19th century. In the 19th and 20th centuries, seven major pandemics are recognized. The first six pandemics occurred during the following periods: 1st: 1816–1826, 2nd: 1829–1851, 3rd: 1852–1860, 4th: 1863–1875, 5th: 1881–1896, 6th: 1899–1923. These pandemics all started in Asia, passed through Europe and then reached South America. The Classical biotype was involved. The seventh pandemic, still in course, started in 1961 in the Celebes Isles, in Asia. In the 1960s, the disease spread through Asia, in the 1970s reached the Middle East and Africa, and in 1991 streaked violently across South America. Now El Tor has replaced the Classical biotype. El Tor biotype had been detected before, in 1905, but only in the development of the seventh pandemic did this biotype replace the Classical one and become dominant [1,7,8,10,11]. In 1992, a new serovariety (O139), which was coined the Bengal serovariety, was detected for the first time in Bangladesh. This new serovariety quickly spread to India and to southeastern Asia, displacing O1. Although serovariety O1 El Tor has reappeared in 1994 and 1995, the Bengal serovariety still remains the dominant one. The illness caused by serovarieties O139 and O1 are indistinguishable [8,12,13]. In 1991, the seventh pandemic entered South America through the coastal area of Peru. On 23 January, in Chancay, north Peru, Vibrio cholerae O1 El Tor was isolated from patients with cholera symptoms, confirming the disease. In this region, between 24 January and 9 February, 1,859 people were hospitalized and 66 died. From Peru, the disease spread rapidly to other countries in South America. Two routes have been proposed for the entrance of the bacterium in Peru: (1) ballast water from a boat coming from Asia; (2) the El Niño current may have transported zooplankton harboring V. cholerae cells. Shellfish and fish nourishing on this zooplankton became contaminated and the bacterium was transmitted to humans who ate these marine foods [14-17]. The misfortune of people who died in the first months of this disastrous South American cholera epidemic appeared to have unleashed scientists to study the disease harder and, indeed, important Int. J. Environ. Res. Public Health 2010, 7 3662 epidemiological studies were carried out during this outbreak. These studies confirmed that contaminated uncooked food and beverages can also be a vehicle for transmission of cholera. 2.3.4. Genes for toxin and pili protein production The genes responsible for toxin production are harbored in the CTXΦ segment (7–9.7 kb) of the chromosome (only in toxigenic strains). The CTXΦ segment carries at least six genes. In addition to the gene encoding cholera toxin production, this segment (virulence cassette) include an accessory cholera toxin (ace), a zonula occludens toxin (zot), core encoded pilin (cep), and an open reading frame of unknown function. During the replication of the chromosome, the CTXΦ fragment can form an autonomous copy and this can constitute an independent plasmid. The plasmid can give rise to virus-like particles—CTXΦ bacteriophages, which can infect non-toxigenic strains. The CTXΦ segment incorporates into the chromosome of the infected cells which became toxigenic. This process was demonstrated in vitro in cell suspensions and in vivo in the gut of the rat [8,13,19,20]. Epidemic and pandemic strains of V. cholerae contain another chromosomal segment designated as VPI. VPI is 39.5 kb in size and contains two ToxR-regulated genes: a regulator of virulence genes (ToxT) and a gene cluster containing colonization factors, including the toxin co-regulated pili (TCP). The tcp gene encodes for the 20.5-kDa TcpA pili protein. This VPI segment appears to be transferable from V. cholerae O1 to non-O1 strains. V. cholerae O139 strains, like O1, carry the structural genes encoded by the CTX operon and TCP. V. cholerae strains non-O1 or O139 normally lack cholera toxin genes and have never been found to carry TCP. 2.3.5. Ecology of the bacterium and the cycle of the disease V. cholerae non-O1 or O139 strains are common in the environment, especially in estuaries. They have been isolated from many estuarine animals such as birds, frogs, fishes and shellfish, and survive and multiply on the surface of phytoplankton and zooplankton cells [8,21]. V. cholerae O1 and O139 strains are isolable from the environment only in epidemic areas. They survive in the cultivable state in water and aquatic and marine organisms for a considerable period of time [8,12,22-24]. When V. cholerae cells face adverse environmental conditions, they reduce cell size, became coccoid and enter a dormant stage inside exopolysaccharide biofilms. Cells display a certain metabolism, but are not able to growth and multiply on the surface of agarized media and give rise to colonies. Cells in this viable but non-culturable state retain viability as well as the potential for pathogenicity for significant periods of time [25-27]. Viable but non-culturable cells can leave their dormant stage and multiply again, resulting in an explosion of their concentration in the environment. Since the presence of non-toxigenic strains is common in aquatic milieu, especially in estuaries, if a horizontal transfer of cholera exotoxin producing genes occurs between toxigenic and non-toxigenic strains, the number of toxigenic cells in the environment can rise rapidly and pronouncedly. The episodic nature and the sudden appearance of violent cholera outbreaks, followed by a rapid slowing down, are probably related with these phenomena. Int. J. Environ. Res. Public Health 2010, 7 3663 3. Salmonellosis 3.1. The Genus Salmonella. Pathogenicity of Main Serovars The genus Salmonella was designated by Lignières in 1900 [28,29]. Antigenic analysis began when Castellani described, in 1902, a method for absorbing antisera. The first antigenic scheme for Salmonella was published by White in 1926, and subsequently developed extensively by Kauffmann, in two classical works published in 1966 and 1978 [28,29]. The Kauffmann-White antigenic scheme contained, by 1988, about 2,250 different serovars [28,29]. The genus Salmonella, a member of the family Enterobacteriaceae, include Gram-negative motile straight rods. Cells are oxidase-negative and catalase-positive, produce gas from D-glucose and utilize citrate as a sole carbon source. Salmonellae have several endotoxins: antigens O, H and Vi [28,29]. The concept ―one serovar-one species‖, in use for many years, is no longer acceptable. The taxonomy and nomenclature of the genus Salmonella has been subject of debate since Le Minor and Popoff proposed changes in a paper published in 1987. The issue was settled by a decision of the International Committee on the Systematics of Prokaryotes and published in 2005. The current taxonomy of the genus is presented in Table 4. According to the rules of bacterial nomenclature, the names of the serovars are not italicized and the first letter must be a capital [28-30]. S. enterica subsp. enterica serovar Enteritidis is the most frequently isolated serovar from humans all over the world. However, locally, other serovars can be predominant. In the period 1994–2004, Tunisia was exposed to salmonellosis outbreaks in 1997, 1999, 2002 and 2004. In 1997, salmonellosis outbreak was caused by serovar Mbandaka. In 1999, three salmonellosis outbreaks were reported from hospitals located in three different regions. Each outbreak was associated with a different serotype: Mbandaka, Livingstone and Typhi Vi+. In 2002, a S. enterica subsp. enterica serovar Livingstone infection occurred in the same hospital that reported an outbreak caused by serovar Typhi Vi+ in 1999, but in a different unit. In that year, the Livingstone serovar jumped to the first position in human infection in Tunisia. In 2004, a second outbreak by serovar Typhi Vi+ was reported. The source of isolation was a fermented juice traditionally extracted from palm-tree. 3.2. Characterization of the Diseases Salmonellae pathogenic to humans can cause two types of salmonellosis: (1) typhoid and paratyphoid fever (do not confuse with typhus, a disease caused by a rickettsia); (2) gastroenteritis. Low infective doses (less than 1,000 cells) are sufficient to cause clinical symptoms. Salmonellosis of newborns and infants presents diverse clinical symptoms, from a grave typhoid-like illness with septicemia to a mild or asymptomatic infection. In pediatric wards, the infection is usually transmitted by the hands of staff. Int. J. Environ. Res. Public Health 2010, 7 3664 Table 4. Current taxonomy and nomenclature of the genus Salmonella. Habitat and pathogenicity of main serovars a. Main serovars Species Sub-species (from a total of ca. Habitat and pathogenicity 1,443) Salmonella Salmonella Abortusovis Pathogenic to sheeps. enterica enterica subsp. Choleraesuis Pathogenic to humans and animals. enterica Enteritidis Ubiquitous and frequently the cause of infections in humans and animals. Very frequent agent of gastroenteritis in humans. Gallinarum Isolated chiefly from chickens and other birds. Causal agent of fowl thyphoid. Paratyphi A Pathogenic only to humans. Causes paratyphoid fever. Paratyphi B Causes paratyphoid fever in humans and very rarely infects animals. Paratyphi C Causes paratyphoid fever in humans. Typhi Pathogenic only to humans, causing typhoid fever. Transmitted by water and food contaminated with feces. Typhimurium Ubiquitous and frequently the cause of infections in humans and animals. Very frequently, the causal agent of gastroenteritis in humans. Typhisuis Pathogenic to swines. Salmonella At least 94 serovars. enterica subsp. arizonae Salmonella At least 323 serovars. enterica subsp. diarizonae Salmonella At least 70 serovars. enterica subsp. Isolated mainly from cold-blooded animals and from houtenae the environment. Not pathogenic to humans. Salmonella At least 11 serovars. enterica subsp. indica Salmonella At least 488 serovars. enterica subsp. salamae Salmonella At least 20 serovars. bongori a Adapted from. Nomenclature according to. Food-borne Salmonella gastroenteritis are frequently caused by ubiquitous Salmonella serovars such as Typhimurium. About 12 h following ingestion of contaminated food, symptoms (diarrhea, vomiting and fever) appear and last 2–5 days. Spontaneous cure usually occurs. Salmonella may be associated with all kinds of food. Prevention of Salmonella food-borne infection relies on avoiding Int. J. Environ. Res. Public Health 2010, 7 3665 contamination (improvement of hygiene), preventing multiplication of Salmonella in food (constant storage of food at 4 °C), and use of pasteurization (milk) or sterilization when possible (other foods). Vegetables and fruits may carry Salmonella when contaminated with fertilizers of fecal origin, or when washed with polluted water. The incidence of typhoid fever decreases when the level of development of a country increases (i.e., controlled water sewage systems, pasteurization of milk and dairy products). Where these hygienic conditions are missing, the probability of fecal contamination of water and food remains high and so is the incidence of typhoid fever. 3.3. Ecology of Salmonellae and the Cycle of Salmonellosis The principal habitat of Salmonella is the intestinal tract of humans and animals. Salmonellae are constantly found in environmental samples, because they are excreted by humans, pets, farm animals, and wild life. Municipal sewage, agriculture pollution, and storm water runoff are the main sources of these pathogens in natural waters [1,32]. Salmonellae do not seem to multiply significantly in the natural environment, but they can survive several weeks in water and in soil if conditions of temperature, humidity, and pH are favorable. Salmonellae isolated from environmental sources are predominantly non-Typhi or Paratyphi serovars. In a study carried out in Tunisia during 1994–2004, S. enterica subsp. enterica serovars Anatum, Enteritidis and Corvallis were the most common serotypes isolated from food. The great majority of the strains were isolated from poultry, red meat, milk and dairy products, vegetables and fruits. From environmental sources, 73% of the isolates were from tap water. Serovars Corvallis, Enteritidis, and Anatum were the commonest. Arvanitidou et al. reported a comparative study carried out in Rivers Aliakmon and Axios, in northern Greece, during a 1-year period, from May 2002 to April 2003. A total of 29 Salmonella species were recovered from the water samples. Many of the isolated Salmonella serovars were of non-human animal origin such as Mbantaka, Virchow, Hadar, Infantis and Senftenberg, commonly isolated from poultry farm. Unlike cholera, humans infected with salmonellae can carry the bacteria in the gut without signs of disease. Infected humans can harbor the bacteria for considerable periods of time. About 5% of patients clinically cured from typhoid fever remain carriers for months or even years. These people can be chronic holders of the bacterium in the gut, and constitute the main reservoir of the bacteria in the environment. The salmonellosis cycle in the environment can involve shellfish. Salmonellae survive sewage treatments if suitable germicides are not used in sewage processing. If effluent from the sewage plant passes into a coastal area, edible shellfish (mussels, oysters) can become contaminated. Shellfish concentrate bacteria as they filter several liters of water per hour. Ingestion by humans of these seafoods (uncooked or superficially cooked) may cause typhoid fever or other salmonellosis. Evidence of such a cycle has been obtained by the use of strain markers, including phage typing. Int. J. Environ. Res. Public Health 2010, 7 3666 4. Shigellosis or Bacillary Dysentery 4.1. The Genus Shigella Shigella are Gram-negative, non-sporeforming, non-motile, straight rod-like members of the family Enterobacteriaceae. Cells ferment sugars without gas production. Salicin, adonitol and myo-inositol are not fermented. Cells do not utilize citrate, malonate and acetate as sole carbon source and do not produce H2S. Lysine is not decarboxylated. Cells are oxidase-negative and catalase-positive. Members of the genus have a complex antigenic pattern, and taxonomy is based on their somatic O antigens [1,33,34]. Table 5. Current taxonomy and nomenclature of the genus Shigella. Habitat and pathogenicity of species a. Species Main serotypes Habitat and pathogenicity Shigella dysenteriae 15 serotypes. Intestinal pathogens of humans and primates, causing bacillary dysentery. Humans are the primary reservoir. A long-term carrier Shigella flexneri 8 serotypes state occurs in few cases. 9 subserotypes Shigella dysenteriae serotype 1 causes more severe disease then other serotypes and produces a potent Shigella boydii 19 serotypes exotoxin (Shiga toxin). Large epidemics in developing countries are commonly caused by serotype 1. Diseases caused by other serotypes may be mild or severe. Shigella sonnei illness is usually milder than that Shigella sonnei 1 serotype caused by other Shigella species. a Adapted from. Nomenclature according to. 4.2. Characterization of the Disease The incubation period is 1–4 days. The disease usually begins with fever, anorexia, fatigue and malaise. Patients display frequent bloody stools of small volume (sometimes grossly purulent) and abdominal cramps. Twelve to 36 hours later, diarrhea progresses to dysentery, blood, mucus and pus appearing in feces that decreases in volume (no more than 30 mL of fluid per kg per day) [34-36]. Although the molecular basis of shigellosis is complex, the initial step in pathogenesis is penetration of the colonic mucosa. The resulting focus of Shigella infection is characterized by degeneration of the epithelium and by an acute inflammatory colitis in the lamina propria. Ultimately, desquamation and ulceration of the mucosa cause leakage of blood, inflammatory elements, and mucus into the intestinal lumen. Under these conditions the absorption of water by the colon is inhibited and the volume of stool is dependent upon the ileocecal flow. As a result, the patient will pass frequent, scanty, dysenteric stools [37,38]. In order for Shigella to enter an epithelial cell, the bacterium must first adhere to its target cell. Generally, the bacterium is internalized via an endosome, which it subsequently lyses to gain access to the cytoplasm where multiplication occurs [37,38]. Int. J. Environ. Res. Public Health 2010, 7 3667 4.3. Virulence Factors S. dysenteriae serotype 1 produces high levels of a cytotoxic Shiga toxin. S. sonnei and S. flexneri produce much lower amounts of this toxin. Shiga toxin binds to Galotl-4Galp (galabiose) glycolipid receptors and inhibits mammalian protein synthesis by cleaving the N-glycosidic bond at adenine 4324 in 28S rRNA. The toxic mechanism is identical to that of the plant toxin ricin, produced by Ricinus communis. Shigella also release a LPS endotoxin (O antigens), that cause an inflammatory response [37,38]. Shigella 180- to 230-kb plasmids encode genes essential for virulence, namely for: production of adhesins involved in the adherence of bacteria onto the surface of target epithelial cells; production of invasion plasmid antigens (Ipa) that have a direct role in the Shigella invasion process; transport or processing functions that ensure the correct surface expression of the Ipa proteins; induction of endocytic uptake of bacteria and disruption of endocytic vacuoles; regulation of plasmid-encoded virulence genes [37,38]. Shigella emerged from E. coli during evolution. The acquisition and evolution of the pathogenicity island which encodes all of the genes required for cell invasion and phagolysosomal lysis, permitted a major alteration in pathogenesis [37,38]. 4.4. Risk Factors Many studies have identified risk factors and protective effects for shigellosis incidence and fatality. Despite gradual improvements in water supply, shigellosis continues to be endemic among the disadvantaged populations living in the tropics, often among displaced populations following natural disasters and political crises. In Guatemala, young children, the elderly, and 15–44-year-old males were found to be most susceptible to S. dysenteriae serotype 1. In Sierra Leone, the attack rate was higher among children younger than 5 years of age than in the rest of the population. In rural Bangladesh, shigellosis was most common in children aged 1–2 years and in people 60 years or older. In Dhaka, Bangladesh, it was found that shigellosis mortality was most common in severely malnourished people of all ages, in children under 2 who were not being breastfed, and in all children under 1. In a 3-year study carried out in Matlab, Bangladesh, during 1992 to 1994, it was found that the incidence of S. dysenteriae serotype 1 and S. flexneri was highest in children under 2 followed by children from 2 to 5. The location of S. dysenteriae serotype 1 risk varies in time but S. flexneri risk areas were persistent in time. Neighborhoods near bazaars with many non-septic latrines were at highest risk for S. dysenteriae serotype 1. S. flexneri was most common in flood-controlled areas. It was concluded that S. dysenteriae serotype 1 risk was more related to hygiene and sanitation whereas S. flexneri was more related to the environment. 4.5. Shigellosis through the World The total number of Shigella episodes that occur each year throughout the World is estimated to be 164.7 million, including 163.2 million cases in developing countries, 1.1 million of which result in death. Children under 5 account for 61% of all deaths attributable to shigellosis [35,36]. Int. J. Environ. Res. Public Health 2010, 7 3668 Shigella species are not uniformly distributed in the world. S. dysenteriae is usually found in densely populated areas of South America, Africa and Asia. Infections usually result in significant epidemic outbreaks. Serotype 1 has been distinguished by both its virulence and its ability to produce ravaging epidemics. It predominates in India, Malaysia and Guatemala. Serotype 2 predominates in Yemen and Nigeria. S. flexneri is usually found in areas where endemic shigellosis occurs. S. boydii occurs sporadically, except in the Indian subcontinent where it was first identified. S. sonnei usually occurs in Western developed countries, such as France and USA [35,36]. Important epidemics were reported in the last decades: (1) in 1970 in Central America where 112,000 people were affected and 13,000 died; (2) in 1985, in Texas (USA), 5,000 people became infected after ingestion of contaminated lettuce; (3) in May–June 1994, domestic cases of S. sonnei infection were detected in several European countries, including Norway, Sweden, and the United Kingdom. Epidemiological evidence incriminated imported iceberg lettuce as the vehicle of transmission; (4) in 1996, in Paris, with 153 reported patients. 4.6. Ecology of Shigellae and the Cycle of Shigellosis Shigella is typically an inhabitant of the intestinal tract of humans and other primates [1,33,34,36,39]. It is typically spread by fecal-contaminated drinking water or food, or by direct contact with an infected person. In water, shigellae can survive for at least six months at room temperature, and this high survival favors transmission through water. Flies have been implicated on the transmission of Shigella cells from human feces to foods. The hand is an important vehicle for transmission of shigellosis, since S. dysenteriae serotype 1 cells survives for up to one hour on a human‘s skin and a very small inoculum is required to unchain infection and disease. Indeed, studies on American volunteers experimentally infected with Shigella have shown that as few as one hundred Shigella cells given orally cause the disease in 25–50% of the cases. Resistance of Shigella to gastric juice certainly accounts, although not exclusively, for this high infectivity [36,40]. Asymptomatic and inappropriately-treated patients with shigellosis can harbor the bacteria in the gut and these appear to be the main reservoirs of the bacteria in the environment. Recent reports on the ecology of shigellae have brought new elements for the understanding of the cycle of the disease. In environmental waters of regions with high numbers of shigellosis‘ cases, it has been found that, although numbers of cultivable cells were low, genetic elements such as plasmids and genetic fragments with bacteriophage origin, could be detected. Many of the genes that code for exotoxin production are precisely found in these genetic elements. These results suggest that the sudden rise of the number of virulent strains in the environment can result from the incorporation, by cells with reduced virulence, of this type of genetic elements present in the waters. If this hypothesis is confirmed, there is a certain similarity between the cholera and the shigellosis cycles in the environment. It remains to be elucidated if shigellae can also exist in environmental waters in a viable but non-culturable state, as vibrios. Int. J. Environ. Res. Public Health 2010, 7 3669 5. Pathogenic Escherichia coli Strains E. coli strains isolated from intestinal diseases have been grouped into at least six different main groups, based on epidemiological evidence, phenotypic traits, clinical features of the disease and specific virulence factors. From these, enterotoxigenic (ETEC, namely O148), enterohemorrhagic (EHEC, namely O157) and enteroinvasive serotypes (EIEC, namely O124) are of outstanding importance and can be transmitted through contaminated water [42,43]. 5.1. Enterotoxigenic E. coli (ETEC) Strains Enterotoxigenic E. coli (ETEC) serotypes can cause infantile gastroenteritis. The number of reports of their occurrence in developed countries is comparatively small, but it is an extremely important cause of diarrhea in the developing world, where there is no adequate clean water and poor sanitation. In developing countries, these strains are the most commonly isolated bacterial enteropathogen in children below 5 years of age, and account for several hundred million cases of diarrhea and several ten of thousand deaths each year [42-44]. Disease caused by ETEC follows ingestion of contaminated food or water and is characterized by profuse watery diarrhea lasting for several days that often leads to dehydration and malnutrition in young children [42-44]. ETEC also are the most common cause of ―travelers‘ diarrhea‖ that affects individuals from industrialized countries travelling to developing regions of the World [42-44]. 5.2. Enterohemorrhagic E. coli (EHEC) Strains Reported outbreaks had been associated mainly with the consumption of contaminated foods, such as raw or undercooked ground meat products and raw milk. The primary reservoir of this bacterium has been found to be healthy cattle [42,45,46]. E. coli serotype O157:H7 causes abdominal pain, bloody diarrhea, and hemolytic uremic syndrome. This bacterium produces Shiga-like toxins. The incubation period is 3–4 days, and the symptoms occur for 7–10 days. It is estimated that 2–7% of E. coli O157:H7 infections result in acute renal failure [42,45,46]. Although E. coli O157:H7 is not usually a concern in treated drinking water, outbreaks involving consumption of drinking water contaminated with human sewage or cattle feces have been documented. An increasing number of outbreaks are associated with the consumption of fruits and vegetables (sprouts, lettuce, coleslaw, salad) contaminated with feces from domestic or wild animals at some stage during cultivation or handling. EHEC has also been isolated from bodies of water (ponds, streams), wells and water troughs, and has been found to survive for months in manure and water-trough sediments [45,46]. Person-to-person contact is an important mode of transmission through the oral-fecal route. An asymptomatic carrier state has been reported, where individuals show no clinical signs of disease but are capable of infecting others [45,46]. Int. J. Environ. Res. Public Health 2010, 7 3670 5.3. Enteroinvasive E. coli (EIEC) Strains Enteroinvasive E. coli (EIEC) behave in many respects like shigellae. They are capable of invading and multiplying in the intestinal epithelial cells of the distal large bowel in humans. The illness is characterized by abdominal cramps, diarrhea, vomiting, fever, chills, a generalized malaise, and the appearance of blood and mucus in the stools of infected individuals. [42,43,47]. EIEC strains were isolated, for instance, from 28 subjects in the Jesreel district of Israel during a peak period for dysentery. An investigation in Croatia showed that E. coli O124 could frequently be isolated from cases of gastroenteritis, enterocolitis, and dysentery. The dysentery was more common among the older age groups, while the two other types of disease occurred equally in all age groups. A 1985 survey was carried out in Bankok, Thailand in which 410 children with diarrhea and an equal number of control children without diarrhea were examined for the presence of strains of Shigella, EIEC, and other pathogens. It was found that 17 of the children with diarrhea and six without yielded EIEC [42,43]. Any food contaminated with human feces from an ill individual, either directly or via contaminated water, could cause disease in others. Outbreaks have been associated with hamburger meat and unpasteurized milk. 6. Emerging Waterborne Bacterial Pathogens The emerging pathogenic bacteria of concern outlined here have the potential to be spread through drinking water, but they do not correlate with the presence of E. coli or with other commonly used drinking water quality indicators, such as coliform bacteria. In most cases, there are no satisfactory microbiological indicators of their presence. More studies are needed in order to understand the real significance and dimension of the diseases caused by water contaminated with these bacteria, and the ecology of these pathogens. 6.1. Mycobacterium avium Complex (Mac) The Mycobacterium avium complex (Mac) consists of 28 serovars of two distinct species: Mycobacterium avium and Mycobacterium intracellulare. The importance of Mac organisms was recognized with the discovery of disseminated infection in immunocompromised people, particularly people with HIV and AIDS. Members of MAC are considered opportunistic human pathogens [45,48]. Mac organisms have been identified in a broad range of environmental sources, including marine waters, rivers, lakes, streams, ponds, springs, soil, piped water supplies, plants, and house dust. Mac organisms have been isolated from natural water and drinking water distribution systems in the USA [45,49,50]. The ubiquitous nature of Mac organisms results from their ability to survive and grow under varied conditions. Mac organisms can proliferate in water at temperatures up to 51 °C and can grow in natural waters over a wide pH range. These mycobacteria are highly resistant to chlorine and the other chemical disinfectants used for the treatment of drinking-water. Standard drinking-water treatments will not eliminate Mac organisms but, if operating satisfactorily, will significantly reduce the numbers that may be present in the source water to a level that represents a negligible risk to the general population. The entryway of these mycobacteria in distribution systems is through leaks. Growth of Int. J. Environ. Res. Public Health 2010, 7 3671 Mac organisms in biofilms is probably important for their continuous presence in distribution systems. Slow growing mycobacteria can be found at densities greater than 4,000 per cm2 in the surface biofilm, creating a potentially high level of exposure. The symptoms encountered with Mac infections result from colonization of either the respiratory or the gastrointestinal tract, with possible dissemination to other locations in the body. Exposure to Mac organisms may occur through the consumption of contaminated food, the inhalation of air with contaminated soil particles, or contact with or ingestion, aspiration, or aerosolization of potable water containing the organisms. With respect to water supplies, infection with M. avium and M. intracellulare has been well documented. Unlike gastrointestinal pathogens, where E. coli can be used to indicate their potential presence, no suitable indicators have been identified to signal increasing concentrations of Mac organisms in water systems. 6.2. Helicobacter pylori Helicobacter pylori has been cited as a major etiologic agent for gastritis and has been implicated in the pathogenesis of peptic and duodenal ulcer disease and gastric carcinoma. However, most individuals that are infected by this pathogen remain asymptomatic. Using culture-based methods, H. pylori has not been isolated from environmental sources, including water [45,51]. On the contrary, molecular methods have been successful in detecting this pathogen. Fluorescence in situ hybridization has been successfully used to detect this pathogen in drinking water distribution systems and other water bodies. Polymerase chain reaction has also been used to detect the presence of H. pylori DNA in drinking water, especially associated with biofilms [45,51,52]. In drinking-water biofilms, H. pylori cells rapidly lose culturability, entering a viable but non-culturable state. In these biofilms, cells can persist for more than one month, with densities exceeding 106 cells per cm2. How the organism is transmitted is still not fully understood. However, the fact that it has been recovered from saliva, dental plaques, the stomach, and fecal samples strongly indicates oral-oral or fecal-oral transmission. Water and food appear to be of lesser direct importance, but they can still play a significant role in situations with improper sanitation and hygiene. 6.3. Aeromonas hydrophyla In recent years, A. hydrophila has gained public health recognition as an opportunistic pathogen. It has been implicated as a potential agent of gastroenteritis, septicemia, meningitis, and wound infections. It can play a significant role in intestinal disorders in children under five years old, the elderly, and immunosuppressed people. [45,53,54]. Aeromonas hydrophila are Gram-negative, non-sporeforming, rod-shaped, facultative anaerobic bacilli belonging to the family Aeromonadaceae. Although A. hydrophila is usually the dominant species, other aeromonads, such as A. caviae and A. sobria, have also been isolated from human feces and from water sources [45,54]. Aeromonas species, including A. hydrophila, are ubiquitous in the environment. It is frequently isolated from food, drinking water, and aquatic environments [45,53,54]. In clean rivers and lakes, Int. J. Environ. Res. Public Health 2010, 7 3672 concentrations of Aeromonas spp. are usually around 102 colony-forming units (CFU)/mL. Groundwaters generally contain less than 1 CFU/mL. Drinking water immediately leaving the treatment plant has been found to contain between 0 and 102 CFU/mL. Drinking water in distribution systems can display higher Aeromonas concentrations, due to the growth in biofilms [45,55]. Aeromonas spp. have been found to grow between 5 °C and 45 °C [44,54]. A. hydrophila is resistant to standard chlorine treatments, probably surviving inside biofilms. The common routes of infection suggested for Aeromonas are the ingestion of contaminated water or food or contact of the organism with a break in the skin. Drinking or natural mineral water can be a possible source of contamination for humans. No person-to-person transmission has been reported [45,54]. 7. Microbiological Water Analysis 7.1. The Rationale of the Use of Fecal Indicator Bacteria The most important bacterial gastrointestinal diseases transmitted through water are cholera, salmonellosis and shigellosis. These diseases are mainly transmitted through water (and food) contaminated with feces of patients. Drinking water can be contaminated with these pathogenic bacteria, and this is an issue of great concern. However, the presence of pathogenic bacteria in water is sporadic and erratic, levels are low, and the isolation and culture of these bacteria is not straightforward. For these reasons, routine water microbiological analysis does not include the detection of pathogenic bacteria. However, safe water demands that water is free from pathogenic bacteria. The conciliation of the two needs was met by the discovery and testing of indicator bacteria. Water contaminated with pathogenic species also has the normal inhabitants of the human intestine. A good bacterial indicator of fecal pollution should fulfill the following criteria: (1) exist in high numbers in the human intestine and feces; (2) not be pathogenic to humans; (3) easily, reliably and cheaply detectable in environmental waters. Additionally, the following requisites should be met if possible: (4) does not multiply outside the enteric environment; (5) in environmental waters, the indicator should exist in greater numbers than eventual pathogenic bacteria; (6) the indicators should have a similar die- off behavior as the pathogens; (7) if human fecal pollution is to be separated from animal pollution, the indicator should not be very common in the intestine of farm and domestic animals [1,4,6,57,58]. The usefulness of indicator bacteria in predicting the presence of pathogens was well illustrated in many studies, namely by Wilkes et al.. 7.2. The Composition of Human and Animal Feces Microbiological analysis of the human feces was important in order to structure and validate the use of fecal indicator bacteria in environmental waters. Bacteria present in feces are naturally derived from the microbiota of the human gastrointestinal tract. Although bacteria are distributed throughout the human gastrointestinal tract, the major concentration of microbes and metabolic activity can be found in the large intestine. The upper bowel Int. J. Environ. Res. Public Health 2010, 7 3673 (stomach, duodenum, and jejunum) has a sparse microbiota with up to 105 CFU/ml of contents. From the ileum on, bacterial concentrations gradually increase reaching in the colon 1010 to 1011 CFU/g. It has been estimated that at least 500–1,000 different microbial species exist in the human gastrointestinal microbiota, although on a quantitative basis 10–20 genera usually predominate (Table 6). The total number of microbial genes in the human gastrointestinal tract has been estimated as 2–4 million. This represents an enormous metabolic potential which is far greater than that possessed by the human host [60,64]. Table 6. Total viable count in feces of healthy humans (children, adults and elderly) a. Microbial group Log10 CFU/g feces Bacteroides 11.3* Eubacterium 10.7* Bifidobacterium 10.2* Ruminococcus 10.2* Peptostreptococcus 10.1* Peptococcus 10.0* Clostridium 9.8* Lactobacillus 9.6* Propionobacterium 9.4* Actinomyces 9.2* Methanobrevibacter 8.8* Desulphovibrio 8.4* Fusobacterium 8.4* Enterococci 3.5–7.2** Enterobacteriaceae 5.9–8.0** Escherichia coli 7.5–7.7** Citrobacter 3.3** Klebsiella 2.4** Yeasts 1.0–2.5** a Adapted from [61-63]** and *. * Values expressed as dry weight. ** Values expressed as wet weight. The composition of feces from an individual is stable at genus level, but the species composition can vary markedly from day to day. The relative proportion of intestinal bacterial groups can vary between individuals [60,64]. The microflora of the human gastrointestinal tract is dominated by obligate anaerobes, which are ca. 103 more abundant than facultative anaerobes. The main anaerobic genera are Bacteroides, Eubacterium and Bifidobacteria. These organisms account for ca. 90% of the cultivable human fecal bacteria. Bacteroides (mainly B. thetaiotaomicron and B. vulgatus) are the most abundant organism in the human feces and account for 20–30% of cultivable bacteria. The most abundant facultative anaerobes are Enterococci and Enterobacteriaceae. The main Enterobacteriaceae genera are Escherichia, Citrobacter, Klebsiella, Proteus and Enterobacter. Citrobacter and Klebsiella are present in most individuals although in low numbers. Proteus and Enterobacter are only present in a minority of humans. Int. J. Environ. Res. Public Health 2010, 7 3674 A variety of molecular techniques have been used to study the microbial composition of the human gastrointestinal tract. Results yielded by these studies have shown that many microbes detected by molecular techniques are not isolable by conventional culture-based methods. The presence of high proportions of bifidobacteria detected by culture-based methods is not supported by the results of molecular-based studies. However, the results of molecular-based approaches support many of the findings derived from culture-based methods: the dominance of the obligate anaerobes over facultative anaerobes; the presence of high counts of Bacteroides, Clostridium and Eubacterium. Anaerobic bacteria such as Bacteroides and Eubacterium are not easily cultured by conventional techniques since require incubation chambers with nitrogen atmosphere. Bifidobacterium and Lactobacillus tolerate some oxygen but are fastidious bacteria growing very slowly in culture media. Therefore, these four genera are not adequate to be used as indicators of fecal pollution (the introduction of molecular techniques may improve the situation). Citrobacter, Klebsiella and Enterobacter are present in low numbers in the human intestine and are widespread in environmental waters, and therefore are also not suitable as indicators of fecal pollution. Clostridium, Streptococcus and Escherichia do not suffer from these drawbacks. Therefore, their suitability as fecal indicators has been tested since several decades. 7.3. Fecal Bacteria in Their Hosts and in the Environment 7.3.1. Bacteroides The traditional genus Bacteroides included Gram-negative, non-sporeforming, anaerobic pleiomorphic rods. Many species have been transferred to other genera—Mitsuokella, Porphyromonas, Prevotella, Ruminobacter. Bacteroides are the most abundant bacteria in human feces. In animal feces, on the contrary, Bacteroides are present at low numbers. Although anaerobic, Bacteroides are among the most tolerant to oxygen of all anaerobic human gastrointestinal species. B. thetaiotaomicron is one of the most abundant species in the lower regions of the human gastrointestinal tract. Bacteroides have a high pathogenic potential and account for approximately two-thirds of all anaerobes isolated from clinical specimens. The most frequently isolated species has been B. fragilis. The survival of Bacteroides in environmental waters is usually much lower than the survival of coliforms [64,65]. 7.3.2. Eubacterium The traditional genus Eubacterium included anaerobic non-sporeforming Gram-positive rods. Some species have been transferred to other genera—Actinobaculum, Atopobium, Collinsella, Dorea, Eggerthella, Mogibacterium, Pseudoramibacter and Slackia. Cells are not very aerotolerant. Species isolated from the human gastrointestinal tract include: E. barkeri, E. biforme, E. contortum, E. cylindrioides, E. hadrum, E. limosum, E. moniliforme, E. rectal and E. ventricosum. Int. J. Environ. Res. Public Health 2010, 7 3675 7.3.3. Bifidobacterium Bifidobacteria are Gram-positive, non-sporeforming, pleiomorphic rods. Bifidobacteria are anaerobic (some species tolerate oxygen in the presence of carbon dioxide) or facultative anaerobic. The optimum growth temperature is 35–39 °C. The genus Bifidobacterium contains ca. 25 species, most of which have been detected in the human gastrointestinal tract [64-66]. Bifidobacteria are present in high numbers in the feces of humans and some animals. Several Bifidobacterium species are specific either for humans or for animals. B. cuniculi and B. magnum have only been found in rabbit fecal samples, B. gallinarum and B. pullorum only in the intestine of chickens and B. suis only in piglet feces. In human feces, the species composition changes with the age of the individual. In the intestine of infants B. breve and B. longum generally predominate. In the adult, B. adolescentis, B. catenulatum, B. pseudocatenulatum and B. longum are the dominant species. In both human and animal feces, bifibobacteria are always much more abundant than coliforms [64-66]. Bifidobacteria have been found in sewage and polluted environmental waters, but appears to be absent from unpolluted or pristine environments such as springs and unpolluted soil. This results from the fact that upon introduction into the environment, bifidobacteria decrease appreciably in numbers, probably due to their stringent growth requirements. Bifidobacteria grow poorly below 30 °C and have rigorous nutrient requirements. Reports on the survival of bifidobacteria in environmental waters indicate that their survival is lower than that of coliforms [64-66]. The presence of bifidobacteria in the environment is therefore considered an indicator of fecal contamination. Since some species are specific for humans and animals, the identification of Bifidobacterium species present in the polluted water could, in principle, provide information on the origin of fecal pollution [64-66]. A study carried out in a highly contaminated stream near Bologna, Italy, revealed that B. adolescentis, B. catenulatum, B. longum, B. pseudocatenulatum and B. thermophilum were the most representative species, whereas B. angulatum, B. animalis subsp. animalis (B. animalis), B. breve, B. choerinum, B. minimum, B. pseudolongum subsp. globosum (B. globosum) and B. subtile occurred only in low numbers. Bifidobacteria are the less studied of all fecal bacteria, due to the technical difficulties in their isolation and cultivation. Other Gram-positive bacteria, such as Streptococcus and Lactobacillus, which may occur in higher numbers than bifidobacteria, can inhibit their growth. Although selective media has been designed for the isolation of bifidobacteria from environmental waters, the outcome is still unsatisfactory, with appreciable numbers of false positives and low recovery percentages [64-66]. 7.3.4. Clostridia The genus Clostridium is one of the largest genera of the prokaryotes containing 168 validly published species. From these, 77 (including C. perfringens) are considered to belong to a united group—Clostridium sensu stricto [64,67,68]. Clostridia are Gram-positive rods, forming endospores. Most of the clostridial species are motile with peritrichous flagellation. Cells are catalase-negative and do not carry out a dissimilatory sulphate reduction. Clostridia usually produce mixtures of organic acids and alcohols from carbohydrates Int. J. Environ. Res. Public Health 2010, 7 3676 and proteins. Many species are saccharolytic and proteolytic. Some species fix atmospheric dinitrogen [64,67,68]. The genus Clostridium includes psychrophilic, mesophilic, and thermophilic species. The major role of these organisms in nature is in the degradation of organic material to acids, alcohols, CO 2, H2, and minerals. Frequently, a butyric acid smell is associated with the proliferation of clostridia. The ability to form spores that resist dryness, heat, and aerobic conditions makes the clostridia ubiquitous [64,67,68]. Most species are obligate anaerobic, although tolerance to oxygen occurs. Oxygen sensitivity restricts the habitat of the clostridia to anaerobic areas or areas with low oxygen tensions. Growing and dividing clostridia will, therefore, not be found in air saturated surface layers of lakes and rivers or on the surface of organic material and soil. Clostridial spores, however, are present with high probability in these environments, and will germinate when oxygen is exhausted and when appropriate nutrients are present [64,67,68]. C. perfringens ferment lactose, sucrose and inositol with the production of gas, produce a stormy clot fermentation with milk, reduce nitrate, hydrolyze gelatin and produce lecithinase and acid phosphatase. The species is divided into five types, A to E, on the basis of production of major lethal toxins [68,69]. C. perfringens appears to be a universal component of the human and animal intestine, since has been isolated from the intestinal contents of every animal that has been studied. Humans carry C. perfringens as part of the normal endogenous flora. The main site of carriage is the distal gastrointestinal tract. The principal habitats of type A are the soil and the intestines of humans, animals, and birds. Types B, C, D, and E appears to be obligate parasites of animals and occasionally are found in humans [68,69]. Clostridium perfringens is the most frequently isolated Clostridium in clinical microbiology laboratories, although it seldom causes serious infections. C. perfringens is isolated from infections in humans and the organism most commonly found in gas gangrene in humans. C. perfringens is most commonly isolated from infections derived from the colonic flora, namely peritonitis or abdominal abscess [68,69]. This organism is a common cause of food poisoning due to the formation of the enterotoxin in the intestine. C. perfringens food poisoning is seldom fatal, being marked by diarrhea and nausea, with no vomiting and no fever [68,69]. Sources yielding C. perfringens include soil and marine sediment samples worldwide, clothing, raw milk, cheese, semi-preserved meat products, and venison. Like E. coli, C. perfringens does not multiply in most water environments and is a highly specific indicator of fecal pollution. Berzirtzoglou et al. reported a comparative study on the occurrence of vegetative cells and spores of Clostridium perfringens in a polluted station of the lake Pamvotis, in rural North-West Greece. The numbers of C. perfringens varied according to the water depth. Sporulated forms were found in all sampling sites with the exception of the surface sampling. Int. J. Environ. Res. Public Health 2010, 7 3677 7.3.5. Lactobacillus Lactobacilli are non-sporeforming Gram-positive long rods. There are more than thirty species in the genus. Most are microaerophillic, although some are obligate anaerobes. Cells are catalase-negative and obtain their energy by the fermentation of sugars, producing a variety of acids, alcohol and carbon dioxide. Lactobacilli have complex nutritional requirements and in agarized media may need the supplementation with aminoacids, peptides, fatty-acid esters, salts, nucleic acid derivatives and vitamins. Lactobacilli very rarely cause infections in humans. 7.3.6. Enterococci Enterococci are Gram-positive, non-sporeforming, catalase-negative ovoid cells. Cells occur singly, in pairs or short chains. Optimal growth for most species is 35–37 °C. Some will grow at 42–45 °C and at 10 °C. Growth requires complex nutrients but is usually abundant on commonly used bacteriological media. Cells are resistant to 40% bile, 0.4% azide, 6.5% sodium chloride, have β-glucosidase and hydrolyze esculin. The enterococci are facultative anaerobic but prefer anaerobic conditions [64,71]. The genus was separated from Streptococcus in the 1980s. Enterococci form relatively distinct groups. Members of such groups exhibit similar phenotypic characteristics and species delimitation can be difficult. The E. faecalis group contains, among others, E. faecalis. The E. avium group contains, among others, E. avium. The E. faecium group contains, among others, E. faecium, E. durans and E. hirae. The E. gallinarum group contains, among others, E. gallinarum [64,71]. Most species are part of the intestinal flora of mammals, reptiles, birds, and other animals. In the human digestive tract, E. faecalis is the prevailing species, although in particular situations, E. faecium may predominate. In poultry, E. cecorum, E. durans, E. faecalis, E. faecium and E. hirae and dominate the intestinal flora [64,71]. Enterococci have been increasingly isolated from a variety of nosocomial and other infections, mainly from the urinary tract and wound infections, bacteremias, and endocarditis [64,71]. Although enterococci are considered only a temporary part of the microflora of plants, in optimal conditions, cells can proliferate on their surfaces. E. casseliflavus, E. faecalis, E. faecium, E. hirae, E. mundtii and E. sulfureus have been isolated from plants. They are generally isolated more often from flowers than from buds or leaves [64,71]. Enterococci are naturally present in many kinds of foods, especially those of animal origin such as milk and milk products, meat and fermented sausages. Enterococci are usually considered secondary contaminants of food, although they often play a positive role in ripening and aroma development of some types of cheeses [64,71]. Although soil is not a natural habitat for enterococci, cells can be found in this habitat due to the transport by rain [64,71]. Environmental waters are not a natural habitat for enterococci and their presence in this milieu is considered the result of fecal pollution. The most common species found in environmental waters are E. durans, E. faecalis, E. faecium and E. hirae, and less commonly, E. avium, E. cecorum, E. columbae and E. gallinarum. However, pristine waters in Finland have been reported to contain E. casseliflavus [64,71]. In environmental samples (compost, sewage effluent, harbor sediments, brackish water and swimming pool water), Pinto et al. reported the isolation of E. casseliflavus, E. durans, E. faecalis, Int. J. Environ. Res. Public Health 2010, 7 3678 E. faecium, E. gallinarum and E. hirae. E. durans, E. faecium and E. hirae were isolated from all sources except from harbor sediments. E. raffinosus was only isolated from compost and swimming pool water. E. faecalis and E. faecium accounted for the vast majority of enterococcal strains. 7.3.7. Escherichia Escherichia, a member of Enterobacteriaceae, are oxidase-negative catalase-positive straight rods that ferment lactose. Cells are positive in the Methyl-Red test, but negative in the Voges-Proskauer assay. Cells do not use citrate, do not produce H2S or lipase, and do not hydrolyze urea. E. coli is a natural and essential part of the bacterial flora in the gut of humans and animals. Most E. coli strains are nonpathogenic and reside harmlessly in the colon. However, certain serotypes do play a role in intestinal and extra-intestinal diseases, such as urinary tract infections. In a study of the enteric bacteria present in the feces of Australian mammals, Gordon and FitzGibbon reported that E. coli was the commonest species, being isolated from nearly half of the species studied. 7.3.8. Citrobacter Citrobacter, a member of Enterobacteriaceae, are motile straight rods. Cells are oxidase-negative, catalase-positive and positive in the Methyl-Red test. Cells use citrate, are negative in the Voges-Proskauer test and do not decarboxylate lysine. In a study of the enteric bacteria present in the feces of Australian mammals, Gordon and FitzGibbon reported the isolation of C. amalonaticus, C. freundii and C. koseri (C. diversus). Citrobacter species can be isolated from different clinical sites. In particular, C. freundii is an intestinal inhabitant of humans that may sometimes have—or acquire—the ability to produce an enterotoxin and thus become an intestinal pathogen. Citrobacter is reported to occur in environments such as water, sewage, soil and food [75,76]. 7.3.9. Klebsiella and Raoultella Klebsiella and Raoultella are Enterobacteriaceae, oxidase-negative catalase-positive non-motile straight rods, surrounded by a capsule. Cells decarboxylate lysine, but are ornithine and arginine dihydrolase negative. Cells grow on KCN, do not produce H2S and ferment most carbohydrates. In humans, K. pneumoniae is present as commensal in the nasopharynx and in the intestinal tract. Klebsiella spp. can cause human diseases, ranging from asymptomatic colonization of the intestinal, urinary, or respiratory tract to fatal septicemia. Klebsiella are mostly considered nosocomial pathogens. K. pneumoniae and Enterobacter aerogenes (K. mobilis) are most frequently involved, although K. oxytoca and R. planticola, and rarely R. terrigena, can be found. In the hospital, the principal reservoir of K. pneumoniae is the gastrointestinal tract of patients. The principal vectors are the hands of personnel [77,78]. In a study of the enteric bacteria present in the feces of Australian mammals, Gordon and FitzGibbon reported the isolation of K. pneumoniae and K. oxytoca. Klebsiellae are ubitiquous in the environment. They have been found in a variety of environmental situations, such as soil, vegetation, or water, and they influence many biochemical and geochemical processes. They have been recovered from aquatic environments receiving industrial wastewaters, Int. J. Environ. Res. Public Health 2010, 7 3679 plant products, fresh vegetables, food with a high content of sugars and acids, frozen orange juice concentrate, sugarcane wastes, living trees, and plants and plant byproducts. They are commonly associated with wood, sawdust, and waters receiving industrial effluents from pulp and paper mills and textile finishing plants (see below). Klebsiella have been isolated from the root surfaces of various plants. K. pneumoniae, K. oxytoca, and R. planticola are all capable of fixing dinitrogen [77,78]. 7.3.10. Enterobacter Enterobacter a member of Enterobacteriaceae, are motile straight rods. Cells are positive in the Voges-Proskauer test VP and in Simmons citrate agar. Cells do not decarboxylate lysine, but are ornithine positive. Malonate is usually utilized and gelatin is slowly liquefied. Cells do not produce H2S, deoxyribonuclease and lipase. In a study of the enteric bacteria present in the feces of Australian mammals, Gordon and FitzGibbon reported the isolation of Enterobacter cloacae subsp. cloacae (E. cloacae), E. cancerogenus (E. taylorae) and E. aerogenes (Klebsiella mobilis). Before the widespread use of antibiotics, Enterobacter species were rarely found as pathogens, but these organisms are now increasingly encountered, causing nosocomial infections such as urinary tract infections and bacteremia. In addition, they occasionally cause community-acquired infections [79,80]. In the USA, the Surveillance and Control of Pathogens of Epidemiological Importance project analyzed 24,179 nosocomial bloodstream infections, from 1995–2002. Enterobacter species were the second most common gram-negative organism, behind Pseudomonas aeruginosa. Both bacteria were reported to each represent 4.7% of bloodstream infections in intensive care units. Enterobacter species represented 3.1% of bloodstream infections in non-intensive care units. Of nearly 75,000 gram-negative organisms collected from intensive care units‘ patients in the USA, between 1993 and 2004, Enterobacter species comprised 13.5% of the isolates. Multidrug resistance increased over time, especially in infections caused by E. cloacae. In the USA, the National Healthcare Safety Network reported a study on healthcare-associated infections between 2006 and 2007. They found Enterobacter species to be the eighth most common cause of healthcare-associated infections (5% of all infections) and the fourth most common gram-negative cause of these infections. Enterobacter cloacae subsp. cloacae (E. cloacae) occurs in the intestinal tracts of humans and animals, in hospital environments, the skin, in water, sewage, soil, meat. Nitrogen-fixing strains have been isolated from the roots of rice plants. E. amnigenus has been mostly isolated from water, but some strains were isolated from clinical specimens from the respiratory tract, wounds and feces. E. asburiae strains were isolated from clinical specimens, mostly urine, respiratory tract, feces, wounds, and blood [79,80]. 7.4. Origin of the Use of Fecal Indicator Bacteria Historically, the design and use of indicators of fecal pollution comes from the end of the 19th to beginning of the 20th century. In 1880, von Fritsch described Klebsiella pneumoniae and K. pneumoniae subsp. rhinoscleromatis (Klebsiella rhinoscleromatis) as micro-organisms characteristically found in human feces. In 1885, Escherich described several microorganisms in Int. J. Environ. Res. Public Health 2010, 7 3680 the feces of newborn and suckling babies. This included a motile, rod-shaped microorganism that caused milk to clot, which was named ―Bacterium coli commune‖. He observed that within a few weeks after birth, this bacterium became the dominant organism in the infant colon. Also in 1885, Percy and Grace Frankland started the first routine bacteriological examination of water in London, using Robert Koch‘s solid gelatin media to count bacteria. In 1891, Percy and Grace Frankland came up with the concept that organisms characteristic of sewage must be identified to provide evidence of potentially dangerous pollution. In 1892, Schardinger proposed that since ―Bacterium coli‖ was a characteristic component of the fecal flora, its presence in water could be taken as an indication of the presence of fecal pollution and therefore of the potential presence of enteric pathogens. Soon after the description of ―Bacterium coli‖, other bacteria were isolated from stools and water—Klebsiella in 1882 and Enterobacter in 1890. By 1893, the ―Wurtz method‖ of enumerating ―Bacterium coli‖, by direct plating water samples on litmus lactose agar, was being used by sanitary bacteriologists. This was based on the concept of acid and gas production (detected by the Durham tube) from lactose as a diagnostic feature. In 1905, MacConkey described his now famous MacConkey‘s broth, which was diagnostic for lactose-fermenting bacteria tolerant of bile salts. Coliforms were already considered to be a heterogeneous group of organisms, many of which were not of fecal origin. The origins of the critical observation that ―Bacterium coli‖ was largely fecal in origin while other coliforms were not, could be claimed by Winslow and Walker in 1907. Various classification schemes for coliforms have emerged. The earliest were those of MacConkey in 1909, which recognized 128 different coliform types, while Bergey and Deehan in 1908, identified 256. By the early 1920s, differentiation of coliforms had come to a series of correlations that suggested that indole production, gelatin liquefaction, sucrose fermentation and the Voges–Proskauer reaction were among the more important tests for determining fecal contamination. These developments culminated in the IMViC (Indole, Methyl Red, Voges–Proskauer and Citrate) tests for the differentiation of so-called fecal coliforms, soil coliforms and intermediates. 7.5. Fecal Indicator Bacteria 7.5.1. Coliforms Total coliforms are Gram-negative, oxidase-negative, non-sporeforming rods, that ferment lactose with gas production at 35–37 °C, after 48h, in a medium with bile salts and detergents [1,4,6,57,84]. When the test of coliforms is carried out with environmental waters, several species of the four Enterobacteriaceae genera Escherichia, Klebsiella, Enterobacter and Citrobacter give positive results and therefore are coliforms according to this definition. However, the environmental significance of these four genera is very disparate as discussed in the present text. Therefore, total coliform counts are not necessarily a measure of fecal pollution and indeed can have no relation with this cause [1,4,6,84]. Fecal coliforms (or thermotolerant coliforms) are traditionally defined as coliforms that ferment lactose at 44.5 °C in a medium with bile salts [1,4,57,84]. The range of species detected by the experimental procedure is much lower than that of total coliforms. With environmental polluted waters, only E. coli, and K. oxytoca and K. pneumoniae gave positive results in the test. Traditional tests for total and fecal coliforms are carried out either by the multiple-tube fermentation technique or by filtration through membrane. The multiple-tube fermentation technique is Int. J. Environ. Res. Public Health 2010, 7 3681 used for medium or highly contaminated waters, and the filtration through membrane for low or very low contaminated waters. Filtration through membrane is a very sensitive technique since can detect one (culturable) cell in 500 or even 1,000 mL of water. However, both methods take several days to complete and do not detect viable but non-culturable bacteria [3,57,86]. These limitations stimulate the discovery of alternative methods, faster and, if possible, less prone to false negative results such as those caused by the viable but non-culturable bacteria. The detection of β-D-galactosidase activity (at 37 °C) is usually a good marker for total coliforms in environmental waters, since most of these bacteria display this enzymatic activity [1,3,57,87-91]. Most Escherichia, Citrobacter, Enterobacter, Klebsiella and Raoultella strains have galactosidase. Hafnia, Serratia and Yersinia also possess this enzymatic activity. Most Proteus, Salmonella and Edwardsiella strains do not display β-galactosidase [92-95]. Ca. 10% of the coliform strains isolated from the environment do not have an active formic hydrogenolyase (cleaves formate with the formation of CO2) and therefore do not produce gas being undetected by the traditional techniques but are detected by the assay of β-galactosidase activity [57,96,97]. β-galactosidase cleaves lactose in glucose and galactose, and can be detected by using colored or fluorescent markers that change color after enzyme action, such as XGAL (5-bromo-4-chloro-3-indol- β-galactopyranoside) and ONPG (O-nitrophenyl-β-D-galactopyranoside) or MUGAL (4-methylumbelliferyl-β-D-galactopyranoside), respectively [57,96,97]. In environmental waters, the presence of Aeromonas or Vibrio cholerae can be a source of false positives in the β-D-galactosidase assay, since these bacteria have galactosidase, but are not coliforms [93,95,96,98]. Additionally, in particular environments, such as estuaries, β-galactosidase activity can overestimate total coliform count due to UV-stimulated enzymatic activity in certain bacteria such as E. coli. The detection of β-D-glucuronidase activity (at 44.5 °C) is, generally, a good marker for fecal coliforms in environmental polluted waters and very specific for E. coli [13,85,87-91,97,99-101]. In Gram-negative bacteria, this enzymatic activity if found in most E. coli strains and in some Salmonella and Shigella strains [92-95,97,102]. Aeromonas, Citrobacter, Enterobacter, non-coli Escherichia, Hafnia, Klebsiella, Proteus, Serratia, Vibrio, Yersinia, and most Salmonella strains do not display β-glucuronidase activity [93-95,101,102]. β-D-glucuronidase activity can be detected by using colored or fluorescent markers that change color after enzyme action, such as XGLUC (5-bromo-4-chloro-3-indoxyl-β-D-glucuronide), IBDG (indoxil-β-glucuronide), and MUGLU (4-methylumbelliferyl-β-D-glucuronide), respectively [57,97,100]. The presence of this enzyme in some strains of Bacteroides, Flavobacterium, Staphylococcus, Streptococcus, in anaerobic corynebacteria and Clostridium, has also been reported [93,95-97,102]. β-D-glucuronidase activity in fecal bacteria other then E. coli (Bacteroides, bifidobacteria, clostridia, enterococci and Lactobacillus) is very limited. Although all these glucuronidase positive bacteria could lead to false positive detections in the fecal coliform test, experimental results for environmental polluted waters indicate a significant correlation between fecal coliform detection using conventional techniques and the glucuronidase assay, suggesting that false positives are not significant [85,96]. The detection of total coliforms and fecal coliforms by enzymatic methods are much less time consuming than traditional techniques. With fluorescent markers and the use of a spectrofluorimeter the detection of coliforms can be performed in minutes [57,101]. However, in very low contaminated waters, Int. J. Environ. Res. Public Health 2010, 7 3682 enzymatic methods might not be able to detect coliform cells. Moreover, on-line monitoring of glucuronidase activity is currently too insensitive to replace culture based detection of E. coli. Nevertheless, on-line enzymatic methods can be a valuable complementary tool for high temporal resolution monitoring. More research is needed in order to enhance sensitive and lower detection limits of available on-line glucuronidase techniques. The seminal work of Leclerc et al. clarified the diversified roles that coliforms have in the environment and the real meanings of the tests on total coliforms and fecal coliforms. It was shown that Enterobacteriaceae encompass three groups of bacteria with very different roles in the environment. Group I harbored only E. coli. Since this species usually do not survive for long periods outside this environment (but see topic 10), it was considered a good and reliable indicator of fecal pollution (both animal and human). Group II, the ―ubiquitary‖ group, encompassed several species of Klebsiella (K. pneumoniae and K. oxytoca), Enterobacter (Enterobacter cloacae subsp. cloacae, E. aerogenes) and Citrobacter (C. amalonaticus, C. koseri and C. freundii). These bacteria live in the animal and human gut, but also in the environment, and are easily isolated from the soil, polluted water and plants. Their presence in polluted waters does not necessarily indicate fecal pollution. Finally Group III was composed of Raoultella planticola, R. terrigena, Enterobacter amnigenus and Kluyvera intermedia (Enterobacter intermedius), Serratia fonticola, and the genera Budvicia, Buttiauxella, Leclercia, Rahnella, Yersinia, and most species of Erwinia and Pantoea. These bacteria live in fresh waters, plants and small animals. They grow at 4 °C, but not at 41 °C. They are not indicators of fecal pollution, although can be detected in the total coliform test. Leclerc et al. concluded that: (1) in the enterobacteria, E. coli is the only true and reliable indicator of fecal pollution in environmental waters; (2) the traditional total coliform test should be abandoned, since it can detect bacteria that have no connection with fecal pollution; (3) the detection of fecal coliforms must be carried out at 44.5 °C, and positive results confirmed by identification to species levels in order to exclude false positives such as K. pneumoniae. 7.5.2. Streptococci and Enterococci Fecal streptococci also belong to the traditional indicators of fecal pollution. Fecal streptococci are Gram-positive, catalase-negative, non-sporeforming cocci that grow at 35 °C in a medium containing bile salts and sodium azide. Cells hydrolyze esculin [1,4,57]. Azide is a strong inhibitor of the respiratory chain. Since streptococci are one of the very few bacteria that have no respiratory chain, the test is very specific for this group, and false positives are rarely found [104,105]. Fecal enterococci (E. faecalis, E. faecium, E. avium and E. gallinarum) are fecal streptococci that grow in the presence of 6.5% NaCl at 45 °C. Selective media use these particular characteristics in order to separate enterococci from the other streptococci [104,105]. Several studies [104,106] have reported on the microbiological composition of human and animal (cattle, chicken, deer, dog, fowl, goose, and swine) feces. E. faecalis and E. faecium were present in human and animal feces. However, whereas human feces almost have only these two enterococci, in the animals others species co-occur, like E. avium, E. cecorum, E. durans, E. gallinarum and E. hirae. It was concluded that in urban areas where contamination with dog and chicken feces is not likely, the best marker for human fecal pollution was E. faecalis. Int. J. Environ. Res. Public Health 2010, 7 3683 The intestinal enterococci group has been used as an index of fecal pollution. In human feces, the numbers of intestinal enterococci are generally about an order of magnitude lower than those of E. coli (Table 6). Most species do not grow in environmental waters. In this milieu, fecal enterococci are able to survive longer, are more resistant to drying and chlorination, than E. coli [1,84]. Table 7. Bacteria in the feces of farm and domestic warm-blooded animals a. Log10 cells/g wet weight feces Animal Fecal coliforms Fecal streptococci Clostridium perfringens Chicken 5.4 6.1 2.3 Duck 7.5 7.7 - Horse 4.1 6.8 300 Salmonella 0.1–0.67 Shigella 1 a Adapted from Medema et al.. Baudišová reported a comparative study on the survival of total coliforms, fecal coliforms and E. coli, in sterile and non-sterile river water. In sterile water, all bacteria survived for many months. However, in non-sterile conditions (closer to true environmental conditions), the elimination rate of all bacteria was considerably faster. Total coliforms survived the longest and E. coli the shortest. 9.3. Survival in Groundwater Survival of bacteria in groundwater is influenced by several factors, namely the survival in soil, since in order to reach the groundwater bacteria have to percolate through the soil. Generally, survival in soil (and concomitantly in groundwater) is enhanced by low temperatures, high soil humidity, neutral or alkaline soil pH and the presence of organic carbon. Int. J. Environ. Res. Public Health 2010, 7 3691 Table 10. Disappearance rates of fecal bacteria in groundwaters a. Bacterial group Disappearance rate (per day) E. coli 0.063–0.36 Fecal streptococci 0.03–0.24 Clostridium bifermentans spores 0.00 Salmonella enterica subsp. enterica serovar Typhimurium 0.13–0.22 a Adapted from Medema et al.. 10. Which Indicators of Fecal Pollution Should Be Used? Several fecal indicator bacteria in environmental waters are in current use. From these stand out fecal coliforms, E. coli and enterococci [1,6,137]. In environmental waters, most fecal coliform strains are E. coli. In particular situations, the presence of E. coli is definitively not associated with fecal pollution. These situations were firstly detected in some African countries, namely Nigeria, Ivory Coast and New Guinea (although not in others, such as Uganda) [42,99]. Recent studies carried out in temperate zones indicated that E. coli can persist in secondary, nonhost habitats, outside the hot tropical areas, and become naturalized in these habitats. Byappanahalli et al. reported that E. coli could be isolated from coastal temperate forest soils in Indiana (USA). The aquatic alga Cladophora glomerata (L.) from several Lake Michigan beaches was shown to harbor high densities of E. coli. Ishii et al. reported a study on the survival of E. coli in temperate riverine soils of northern Minnesota (USA). Viable E. coli populations were repeatedly isolated from northern temperate soils in three Lake Superior watersheds. Seasonal variation in the population density of soilborne E. coli was observed; the greatest cell densities were found in the summer to fall, and the lowest numbers, occurred during the winter to spring months. Horizontal, fluorophore-enhanced repetitive extragenic palindromic PCR (HFERP) DNA fingerprint analyses indicated that identical soilborne E. coli genotypes, overwintered in frozen soil and were present over time, and that these strains were different from E. coli strains obtained from wildlife commonly found in the studied habitats or river water. Soilborne E. coli strains had HFERP DNA fingerprints that were unique to specific soils and locations. In laboratory studies, naturalized E. coli strains had the ability to grow and replicate to high cell densities, in nonsterile soils when incubated at 30 or 37 °C and survived longer than 1 month when soil temperatures were lower than 25 °C. It was concluded that these E. coli strains became naturalized, autochthonous members of the soil microbial community. In a latter paper, Ksoll et al. studied epilithic periphyton communities at three sites on the Minnesota shoreline of Lake Superior (USA). Fecal coliform densities increased up to 4 orders of magnitude in early summer, and decreased during autumn. HFERP DNA fingerprint analyses indicated that waterfowl (geese, terns, and gulls) were the major primary source of periphyton E. coli strains that could be identified. Periphyton and sewage effluent were also major potential sources. Several periphyton E. coli isolates were genotypically identical, repeatedly isolated over time. Inoculated E. coli rapidly colonized natural periphyton in laboratory microcosms and persisted for several weeks, Int. J. Environ. Res. Public Health 2010, 7 3692 and some cells were released to the overlying water. It was concluded that E. coli had became a naturalized member of the bacterial periphyton communities. The presence, persistence, and possible naturalization of E. coli in these habitats can confound the use of fecal coliforms as a reliable indicator of recent fecal contamination of environmental waters. Future studies should consider other nonhost habitats as potential sources of fecal coliform bacteria in aquatic environments. Considering these limitations, it appears to be advisable, in order to check the microbiological quality of drinking water, to complement the determination of Escherichia coli with the assay of enterococci. This rationale that has been followed, for many years, in the making of the drinking-water legislation in the European Union. However, for many developing countries, where limited financial resources are the norm and reality, the routine determination of these two parameters can be difficult to implement. In these circumstances, it appears common sense that is better to determine a (good) parameter, such as Escherichia coli, than have no analysis done. In this context, USA legislation emerges as a pragmatic approach to the problem. According to the American legislation, total coliforms are the routine parameter to be determined. Only when these determinations are repeatedly positive, it is mandatory to assess fecal coliforms [142,143]. Although total coliforms are not necessarily fecal bacteria, the rationale behind this system is correct, since: (1) a positive test in fecal coliforms (which is our target) is necessarily positive in the total coliform procedure; (2) the inverse is not necessarily true; (3) total coliforms are easily and cheaply assayed in waters. As an alternative to the determination of both E. coli and enterococci, the assay of ammonia in environmental waters can be useful and complement the determination of fecal coliforms. Ammonia is one of the key molecules in the nitrogen cycle. The presence of ammonia in surface waters can be due to direct contamination by agricultural fertilizers, and/or to microbial degradation of proteins, nucleic acids and urea, implying therefore the presence of a considerable concentration of organic matter in the water. Ammonia is rapidly oxidized in the environment and is typically found in natural waters at concentrations less than 0.1 mg/L. Concentrations significantly above this indicate gross contamination by fresh sanitary waste, where ammonia levels are typically very high (tens or hundreds of mg/L). Espigares et al. reported a comparative study of chemical and microbiological indicators (total and fecal coliforms, fecal streptococci and sulphite-reducing clostridia) in a stretch of the Guadalquivir River (Spain) and its affluents. Total coliforms were correlated with fecal coliforms, but were not correlated with fecal streptococci and clostridia. Fecal coliforms were correlated with the other indicators. Fecal streptococci and sulphite-reducing clostridia were correlated with the other indicators except for total coliforms. All these microbiological indicators were correlated with dissolved oxygen (negatively), dissolved organic carbon and ammonia (positively). Cabral and Marques in a study of a polluted river (Febros) in the Great Oporto area (northwest Portugal) found that ammonia was significantly correlated with all the microbiological assayed parameters—total and fecal coliforms, fecal streptococci and enterococci. These correlations are most probably due to the carry-over of organic matter in wastewaters, and to a high microbial ammonification activity. Int. J. Environ. Res. Public Health 2010, 7 3693 Simple and rapid in-field tests and automated and continuous systems are available for the assay of ammonia in environmental waters. More studies are needed in order to confirm the use of ammonia as a reliable parameter in a preliminary screening for emergency fecal pollution outbreaks. 11. Conclusions (1) Safe drinking water for all is one of the major challenges of the 21st century. (2) Microbiological control of drinking water should be the norm everywhere. (3) Routine basic microbiological analysis of drinking water should be carried out by assaying the presence of Escherichia coli by the culture methods. On-line monitoring of glucuronidase activity is currently too insensitive to replace culture based detection of E. coli but is a valuable complementary tool for high temporal resolution monitoring. Whenever financial resources are available, coliform determinations should be complemented with the quantification of enterococci. (4) More studies are needed in order to check if ammonia is reliable for a preliminary screening for emergency fecal pollution outbreaks. (5) Financial resources should be devoted to a better understanding of the ecology and behavior of human and animal fecal bacteria in environmental waters. References 1. WHO (World Health Organization). Guidelines for Drinking-water Quality, Incorporating 1st and 2nd Addenda, Volum