Lab Quiz Prep PDF

DNA Extraction Lab: ------------------- ### Procedure: 1. 2. 3. 4. ### Purpose: 1. 2. 3. 4. Bioengineering Lab: ------------------- ### Procedure: 1. 2. 3. 4. 5. 6. 7. ### Purpose: 1. 2. 3. 4. 5. 6. 7. ### Goals: The overall goal of this process is to successfull...

DNA Extraction Lab: ------------------- ### Procedure: 1. 2. 3. 4. ### Purpose: 1. 2. 3. 4. Bioengineering Lab: ------------------- ### Procedure: 1. 2. 3. 4. 5. 6. 7. ### Purpose: 1. 2. 3. 4. 5. 6. 7. ### Goals: The overall goal of this process is to successfully introduce foreign DNA into bacterial cells to observe genetic transformation. By following the steps, the experiment demonstrates how new traits (e.g., resistance or fluorescence) can be expressed, providing a practical understanding of genetic engineering and its applications in biotechnology, medicine, and research. ### Outcome: The outcome is the successful transformation of bacterial cells with foreign DNA, allowing them to express new traits such as antibiotic resistance or fluorescence. This is confirmed by observing bacterial growth on selective media, demonstrating the practical application of genetic modification in fields like biotechnology and medicine. Electrophoresis Lab: -------------------- ### Procedure: 1. 2. 3. 4. 5. ### Purpose: 1. 2. 3. 4. 5. ### Results: If a rainbow pattern appears in the gel after electrophoresis, it means that the dye mixture has successfully separated into distinct bands, each representing a different dye component. This results in a series of colored bands corresponding to each dye, creating a rainbow-like effect on the gel. The rainbow pattern occurs because the dyes in the mixture have different sizes and charges, causing them to migrate at different rates during electrophoresis. Smaller or more negatively charged dyes move faster, while larger or less charged dyes move slower, creating distinct bands. ### Use or application: Real-world Application: In research and diagnostics, electrophoresis is commonly used for DNA, RNA, and protein analysis, enabling researchers to assess sample integrity and composition. This method could apply to quality control in pharmaceuticals or forensic analysis. The lab works by using an electric current to separate dyes based on their size and charge. Dyes with a stronger negative charge or smaller size move faster, while larger or less negatively charged dyes move slower. Crystal Violet might be a candidate in traveling to the negative (black), as it could have a positive charge under certain conditions.