Lab Activity 2 PDF - Animal Cell Structure and Division

Laboratory Activity 2 Laboratory Worksheet 2: Animal Cell Structure and Division LEARNING OUTCOMES CLO 1. Identify and distinguish between prokaryotic and eukaryotic cells. LO1.1: Differentiate and identify the parts of prokaryotic and eukaryotic cell. LO1.2: Distingui...

Laboratory Activity 2 Laboratory Worksheet 2: Animal Cell Structure and Division LEARNING OUTCOMES CLO 1. Identify and distinguish between prokaryotic and eukaryotic cells. LO1.1: Differentiate and identify the parts of prokaryotic and eukaryotic cell. LO1.2: Distinguish the differences and similarities between mitosis and meiosis. LO1.3: Demonstrate the process of cell division. --------------------------------------------------------------------------------------------------------------------------------------- PRE-LABORATORY QUESTIONS --------------------------------------------------------------------------------------------------------------------------------------- The following questions will help you in preparing for the laboratory activity by ensuring you understand key concepts and techniques related to this topic. (The pre-lab questions are not graded but must be answered or studied to prepare for the pre-lab discussion and laboratory activity) 1. What are the key differences between mitosis and meiosis in terms of their purpose and outcomes? 2. Why is it necessary for organisms to have both mitosis and meiosis as part of their life cycle? 3. How do mitosis and meiosis contribute to growth, repair, and reproduction in living organisms? --------------------------------------------------------------------------------------------------------------------------------------- MATERIALS --------------------------------------------------------------------------------------------------------------------------------------- Materials Qty Specs Item 1 Piece Light microscope Prepared slides Lab kit Lab gown --------------------------------------------------------------------------------------------------------------------------------------- PROCEDURE --------------------------------------------------------------------------------------------------------------------------------------- A. Observing structures of gametes 1. Obtain prepared slide of ovary. 2. Focus the egg cell under scanner, LPO, and HPO. 3. Illustrate the image on your worksheet. 4. Locate the nucleus, plasma membrane, cytoplasm, and other parts you can see. B. Observing mitosis 1. Obtain prepared slides of mitosis. 2. View the specimen under scanner, LPO, and HPO. 3. Locate the different phases: interphase, prophase, metaphase, anaphase, telophase 4. Illustrate the cells as viewed under the microscope. C. Observing meiosis 1. Obtain prepared slides of meiosis. 2. View the specimen under scanner, LPO, and HPO. 3. Locate the different phases: leptotene, zygotene, pachytene, prophase I, diakinesis, metaphase I, anaphase I, telophase I, prophase II, metaphase II, anaphase II, telophase II. 4. Illustrate the cells as viewed under the microscope. NS0400L – FUNDAMENTALS OF ZOOLOGY  Laboratory Activity 2 Observe proper handling of the microscope. Handle microscope slides and coverslips carefully to avoid cuts from broken glass. Do not play with the heat and keep lighter away when not in use. SAFETY Adhere to all laboratory rules and guidelines. PRECAUTIONS All equipment and materials must be cleaned and dried properly. Working table must be cleaned. DISPOSAL AND Dispose waste materials properly according to your lab's disposal protocols. CLEAN-UP Lab Protocols: Mitosis and Meiosis: https://slcc.pressbooks.pub/collegebiologylab/chapter/protocol-mitosis-and- meiosis/ REFERENCES NS0400L – FUNDAMENTALS OF ZOOLOGY  Laboratory Activity 2 LABORATORY REPORT Name: ______________________________________________________ Score: LAB ACTIVITY 2 _______________________________________________________________ _______________________________________________________________ _____/85 Date Performed: ____________________ Group: ________ Date Submitted: _______________________ Results and Observations A. Observing Structure of Gametes Illustrate the ovary cell and label the different parts you are tasked to locate. Describe the specimen in the space provided. (5pts) _______________________________________ _______________________________________ _______________________________________ _______________________________________ _______________________________________ _______________________________________ _______________________________________ _______________________________________ _______________________________________ B. Observing Mitosis Illustrate each phase. Based on the appearance of these cells, defend why do you think these cells are under each phase. (20pts) Interphase: Prophase: NS0400L – FUNDAMENTALS OF ZOOLOGY  Laboratory Activity 2 Metaphase: Anaphase: Telophase: C. Observing Meiosis Illustrate each phase. Based on the appearance of these cells, defend why do you think these cells are under each phase. (40pts) Leptotene: Zygotene: NS0400L – FUNDAMENTALS OF ZOOLOGY  Laboratory Activity 2 Pachytene: Prophase I: Diakinesis: Metaphase I: Anaphase I: Telophase I: NS0400L – FUNDAMENTALS OF ZOOLOGY  Laboratory Activity 2 Prophase II: Metaphase II: Anaphase II: Telophase II: Post-Lab Questions Answer the following questions. Include relevant citations to support your explanation. No citation, no point. (Use the back portion of this paper) 1. Describe the purpose of mitosis in multicellular organisms. (3 pts) ________________________________________________________________________________________________________________________ ________________________________________________________________________________________________________________________ ________________________________________________________________________________________________________________________. 2. Why is it important that sister chromatids separate during mitosis? (3pts) ________________________________________________________________________________________________________________________ ________________________________________________________________________________________________________________________ ________________________________________________________________________________________________________________________. NS0400L – FUNDAMENTALS OF ZOOLOGY  Laboratory Activity 2 3. How does crossing over during prophase I contribute to genetic diversity? (3pts) ________________________________________________________________________________________________________________________ ________________________________________________________________________________________________________________________ ________________________________________________________________________________________________________________________. 4. Compare the end products of meiosis I and meiosis II. (3pts) ________________________________________________________________________________________________________________________ ________________________________________________________________________________________________________________________ ________________________________________________________________________________________________________________________. 5. If a cell with 46 chromosomes undergoes mitosis, how many chromosomes will the daughter cells have? (3pts) ________________________________________________________________________________________________________________________ ________________________________________________________________________________________________________________________ ________________________________________________________________________________________________________________________. Summary and Conclusions In a minimum of 10 sentences, summarize the concepts you learned from this activity (5 pts) _________________________________________________________________________________________________________________________________ _________________________________________________________________________________________________________________________________ _________________________________________________________________________________________________________________________________ _________________________________________________________________________________________________________________________________ _________________________________________________________________________________________________________________________________ _________________________________________________________________________________________________________________________________ _________________________________________________________________________________________________________________________________ _________________________________________________________________________________________________________________________________ _________________________________________________________________________________________________________________________________ _________________________________________________________________________________________________________________________________ _________________________________________________________________________________________________________________________________ _________________________________________________________________________________________________________________________________. NS0400L – FUNDAMENTALS OF ZOOLOGY