Pharmacology 2 Lab 1 PDF

University of Tripoli Faculty of pharmacy Department of pharmacology Pharmacology 2 lab 1 SNS and PSNS effect on blood pressure and heart rate By: Dr. Malak. M. Jafari Edited by : Balsam AL-belazi...

University of Tripoli Faculty of pharmacy Department of pharmacology Pharmacology 2 lab 1 SNS and PSNS effect on blood pressure and heart rate By: Dr. Malak. M. Jafari Edited by : Balsam AL-belazi 2024  a)Diagram of the Langendorff apparatus. Blue: tubing for the physiological solution. Red: tubing for the surrounding heating solution. b) The Langendorff apparatus, pump and aortic cannula feeding into a porcine heart. Isolated innervated rabbit heart mounted to the working heart system. A pair of needle electrodes is placed close to the superior cardiac branch (cathode) and cranial to the cervical VN (anode). ECG leads were placed into the right atrium for heart rate detection VN vagus nerve. The effect of Parasympathetic nerves system on blood pressure and heart rate : Decrease in Decrease in H.R Increase in pulse B.P pressure  Pulse pressure :is the difference between systolic and diastolic blood pressure (width of the blood pressure band on the plot ) If resting blood pressure is (systolic/diastolic) 120/80 millimeters of mercury (mmHg), pulse pressure is 40. Pulse pressure Why?? This is due to greater fall in diastolic than systolic pressure When interval between beats increased there is greater time for blood to run off from the arteries into arterioles and capillaries Small dose of acetylcholine dose not affect heart rate ? acetylcholine selectively dilate arterioles in low doses Why?? because Muscarenic receptors on blood vessels (M3) are more sensitive to acetylcholine than (M2) in heart Why vagal nerve stimulation decrease both HR and BP ? Since the motor fibers of the vagus nerve do not provide any significant innervations to blood vessel, the fall in blood pressure seen in vagal stimulation due entirely to decrease in heart rate High dose of acetylcholine  Pulse B.P H.R pressure  What is the transmitter released from post ganglionic nerve to produce the response shown in cardiac nerve stimulation ?? Noradrenaline Which receptor is responsible for noradrenalin effect on the heart ?? β1 β 1 activation lead to : Increase in cardiac contraction velocity Increase in stroke volume Inject a small dose of noradrenalin ?? M.Jafari M.Jafari Inject a high dose of noradrenaline ? M.Jafari M.Jafari what is the cardiac response for high adrenaline dose ??