Plant Virology PDF
Document Details
Uploaded by Deleted User
Tags
Summary
This document discusses methods for detecting and identifying plant viruses, including techniques like electron microscopy and in vitro studies. It also covers the characteristics and properties of plant viruses and their effects on plant hosts. Information about the sizes and shapes of different viruses is also included.
Full Transcript
in vitro ( in vitro - ) in vitro...
in vitro ( in vitro - ) in vitro (dilution end point, DEP) (~20oC) : pH 7,0) : 1 3. :1 10-1-10-7 ) (thermal inactivation point, TIP) C) TSWV: 45 oC ( TMV: 95 oC ( ) -70 oC (virion) https://www.bath.ac.uk/corporate-information/electron-microscopy/ 80-110 nm, Tospovirus 300x18 nm, Tobamovirus (TMV). (2300oC ( -4 mm Hg).. ( -10 ) (. X (phosphotungstate, PTA) ammonium molybdate) (uranyl acetate, UA) : : : Orthotospovirus EMDV MSV PTA GVA TMV )- )- : : : - ( 30 nm 300 nm ) Cocoa swollen shoot virus, Tobacco mosaic virus, 300 nm X 18 nm 28 x 130 nm Potato virus Y, (12 nm , 1000 nm ) 740 nm 30 x 18 nm (Geminiviridae) : Maize streak virus ) )- Potyvirus) Standard: TMV 300nm x18 nm 0,2
