Histology Practical 3 PDF

# Level-1 Semester-1 HISTOLOGY Practical 3 - Cell Structure **Practical 3 - Cell Structure** (Cell inclusions + Nucleus + Division) **DR. M. YUSUF** ## CELL INCLUSIONS **GLYCOGEN - BEST'S CARMINE** * **Q1: Identify?** → Glycogen inclusions. * **Q2: Mention the stain?** → Best's Carmine * **Q3...

# Level-1 Semester-1 HISTOLOGY Practical 3 - Cell Structure **Practical 3 - Cell Structure** (Cell inclusions + Nucleus + Division) **DR. M. YUSUF** ## CELL INCLUSIONS **GLYCOGEN - BEST'S CARMINE** * **Q1: Identify?** → Glycogen inclusions. * **Q2: Mention the stain?** → Best's Carmine * **Q3: Name the organ?** → Liver. **α-GLYCOGEN-EM** * **Q1: Identify?** → Glycogen inclusions. * **Q2: Mention the type?** → Alpha (α). * **Q3: Name the organ?** → Liver. **B-GLYCOGEN-EM** * **Q1: The arrows point to** → B- Glycogen inclusions. * **Q2: Name the organ?** → Skeletal muscles. ## LIPID INCLUSIONS * **Lipid inclusions (H & E)** * **Lipid inclusions (Osmic acid)** * **Q1: Identify?** → Lipid inclusions. * **Q2: Mention the stain?** → H&E (Lt) + Osmic acid (Rt). ## THE NUCLEUS **THE NUCLEUS - LM** - **Q1: Identify the shape of the nuclei?** * Spherical * Multilobed * Kidney-shaped - **Q2: The arrows point to** → Nucleolus **STAINING AND APPEARANCE OF THE NUCLEUS** * **Vesicular (open-face) nucleus** * **Condensed nucleus** **THE NUCLEUS - EM** | The arrow | Nuclear membrane | |---|---| | 1 | Nucleolus | | 2 | Peripheral chromatin | | 3 | Nuclear sap | **THE NUCLEUS - EM** - **N** - nucleolus - **1** - peripheral chromatin - **2** - nucleolar-associated chromatin - **3** - chromatin islands - **4** - nuclear sap ## PHASES OF MITOSIS **PROPHASE** **METAPHASE** **ANAPHASE** **TELOPHASE** - Cleavage furrow