GIT , Digestion, Absorption & Nutritional Principles.pdf

Physiology Gastrointestinal System Chapter 26 | Digestion, Absorption & Nutritional Principles Gastrointestinal System | Digestion & Absorption Contents : Introduction Carbohydrates Digestion Absorption Protein Digestion Absorption Nucleic acid Lipid Digestion Absorption Iron Absorption Chapter Summ...

Physiology Gastrointestinal System Chapter 26 | Digestion, Absorption & Nutritional Principles Gastrointestinal System | Digestion & Absorption Contents : Introduction Carbohydrates Digestion Absorption Protein Digestion Absorption Nucleic acid Lipid Digestion Absorption Iron Absorption Chapter Summary 3 7 22 32 48 59 62 73 81 97 Gastrointestinal System | Digestion & Absorption Introduction : The gastrointestinal system is the portal through which nutritive substances, vitamins, minerals, and fluids enter the body. Proteins, fats, and complex carbohydrates are broken down into absorbable units (digested), principally, although not exclusively, in the small intestine. The products of digestion and the vitamins, minerals, and water cross the mucosa and enter the lymph or the blood (absorption). Gastrointestinal System | Digestion & Absorption Gastrointestinal System | Digestion & Absorption Enzymes from the salivary glands attack carbohydrates (and fats in some species); enzymes from the stomach attack proteins and fats; and enzymes from the exocrine portion of the pancreas attack carbohydrates, proteins, lipids, DNA, and RNA. Other enzymes that complete the digestive process are found in the luminal membranes and the cytoplasm of the cells that line the small intestine. The action of the enzymes is aided by the hydrochloric acid secreted by the stomach and the bile secreted by the liver. Gastrointestinal System | Digestion & Absorption Most substances pass from the intestinal lumen into the enterocytes and then out of the enterocytes to the interstitial fluid. The processes responsible for movement across the luminal cell membrane are often quite different from those responsible for movement across the basal and lateral cell membranes to the interstitial fluid. Carbohydrates | Digestion Gastrointestinal System | Digestion & Absorption Digestion of Carbohydrates : The principal dietary carbohydrates are polysaccharides, disaccharides, and monosaccharides. Starches (glucose polymers) and their derivatives are the only polysaccharides that are digested to any degree in the human gastrointestinal tract by human enzymes. Amylopectin, which typically constitutes around 75% of dietary starch, is a branched molecule, whereas amylose is a straight chain with only 1:4α linkages. Gastrointestinal System | Digestion & Absorption Gastrointestinal System | Digestion & Absorption The disaccharides lactose (milk sugar) and sucrose (table sugar) are also ingested, along with the monosaccharides fructose and glucose. In the mouth, starch is attacked by salivary α-amylase. The optimal pH for this enzyme is 6.7. However, it remains partially active even once it moves into the stomach, despite the acidic gastric juice, because the active site is protected in the presence of substrate to some degree. Gastrointestinal System | Digestion & Absorption Gastrointestinal System | Digestion & Absorption In the small intestine, both the salivary and the pancreatic α-amylase also act on the ingested polysaccharides. The end products of α-amylase digestion are oligosaccharides: the disaccharide maltose; the trisaccharide maltotriose; and α-limit dextrins, polymers of glucose containing an average of about eight glucose molecules. Gastrointestinal System | Digestion & Absorption The oligosaccharidases responsible for the further digestion of the starch derivatives are located in the brush border of small intestinal epithelial cells. Some of these enzymes have more than one substrate. Isomaltase is mainly responsible for hydrolysis of 1:6α linkages. Along with maltase and sucrase, it also breaks down maltotriose and maltose. Sucrase and isomaltase are initially synthesized as a single glycoprotein chain that is inserted into the brush border membrane. Gastrointestinal System | Digestion & Absorption It is then hydrolyzed by pancreatic proteases into sucrase and isomaltase subunits. Sucrase hydrolyzes sucrose into a molecule of glucose and a molecule of fructose. In addition, lactase hydrolyzes lactose to glucose and galactose. Deficiency of one or more of the brush border oligosaccharidases may cause diarrhea, bloating, and flatulence after ingestion of sugar (Clinical Box). Gastrointestinal System | Digestion & Absorption Gastrointestinal System | Digestion & Absorption The diarrhea is due to the increased number of osmotically active oligosaccharide molecules that remain in the intestinal lumen, causing the volume of the intestinal contents to increase. In the colon, bacteria break down some of the oligosaccharides, further increasing the number of osmotically active particles. The bloating and flatulence are due to the production of gas (CO2 and H2) from disaccharide residues in the lower small intestine and colon. Gastrointestinal System | Digestion & Absorption Gastrointestinal System | Digestion & Absorption Gastrointestinal System | Digestion & Absorption CLINICAL BOX 26–1: Lactose Intolerance In most mammals and in many races of humans, intestinal lactase activity is high at birth, then declines to low levels during childhood and adulthood. The low lactase levels are associated with intolerance to milk (lactose intolerance). Most Europeans and their American descendants retain sufficient intestinal lactase activity in adulthood; the incidence of lactase deficiency in northern and western Europeans is only about 15%. Gastrointestinal System | Digestion & Absorption However, the incidence in blacks, American Indians, Asians, and Mediterranean populations is 70–100%. When such individuals ingest dairy products, they are unable to digest lactose sufficiently, and so symptoms such as bloating, pain, gas, and diarrhea are produced by the unabsorbed osmoles that are subsequently digested by colonic bacteria. Gastrointestinal System | Digestion & Absorption Therapeutic Highlights The simplest treatment for lactose intolerance is to avoid dairy products in the diet, but this can sometimes be challenging (or undesirable for the individual who loves ice cream). Symptoms can be ameliorated by administration of commercial lactase preparations, but this is expensive. Yogurt is better tolerated than milk in intolerant individuals because it contains its own bacterial lactase. Carbohydrates | Absorption Gastrointestinal System | Digestion & Absorption Absorption of Carbohydrates : Hexoses are rapidly absorbed across the wall of the small intestine. Essentially all the hexoses are removed before the remains of a meal reach the terminal part of the ileum. The sugar molecules pass from the mucosal cells to the blood in the capillaries draining into the portal vein. Gastrointestinal System | Digestion & Absorption The transport of glucose and galactose depends on Na+ in the intestinal lumen; a high concentration of Na+ on the mucosal surface of the cells facilitates sugar influx into the epithelial cells while a low concentration inhibits sugar influx into the epithelial cells. This is because these sugars and Na+ share the same cotransporter, or symport, the sodiumdependent glucose transporter (SGLT, Na+ glucose cotransporter). Gastrointestinal System | Digestion & Absorption Gastrointestinal System | Digestion & Absorption The members of this family of transporters, SGLT-1 and SGLT-2, resemble the glucose transporters (GLUTs) responsible for facilitated diffusion in that they cross the cell membrane 12 times and have their –COOH and – NH2 terminals on the cytoplasmic side of the membrane. However, there is no homology to the GLUT series of transporters. SGLT-1 is responsible for uptake of dietary glucose from the gut. Gastrointestinal System | Digestion & Absorption The related transporter, SGLT-2, is responsible for glucose transport out of the renal tubules. Because the intracellular Na+ concentration is low in intestinal cells (as it is in other cells), Na+ moves into the cell along its concentration gradient. Glucose moves with the Na+ and is released in the cell. The Na+ is transported into the lateral intercellular spaces, and the glucose is transported by GLUT2 into the interstitium and thence to the capillaries. Gastrointestinal System | Digestion & Absorption Thus, glucose transport is an example of secondary active transport; the energy for glucose transport is provided indirectly, by the active transport of Na+ out of the cell. This maintains the concentration gradient across the luminal border of the cell, so that more Na+ and consequently more glucose enter. When the Na+/glucose cotransporter is congenitally defective, the resulting glucose/galactose malabsorption causes severe diarrhea that is often fatal if glucose and galactose are not promptly removed from the diet. Gastrointestinal System | Digestion & Absorption Glucose and its polymers can also be used to retain Na+ in diarrheal disease. As indicated, SGLT-1 also transports galactose, but fructose utilizes a different mechanism. Its absorption is independent of Na+ or the transport of glucose and galactose; it is transported instead by facilitated diffusion from the intestinal lumen into the enterocytes by GLUT5 and out of the enterocytes into the interstitium by GLUT2. Some fructose is converted to glucose in the mucosal cells. Gastrointestinal System | Digestion & Absorption Gastrointestinal System | Digestion & Absorption Insulin has little effect on intestinal transport of sugars. In this respect, intestinal absorption resembles glucose reabsorption in the proximal convoluted tubules of the kidneys ; neither process requires phosphorylation, and both are essentially normal in diabetes but are depressed by the drug phlorizin. The maximal rate of glucose absorption from the intestine is about 120 g/h. Proteins | Digestion Gastrointestinal System | Digestion & Absorption Protein Digestion: Protein digestion begins in the stomach, where pepsins cleave some of the peptide linkages. Like many of the other enzymes concerned with protein digestion, pepsins are secreted in the form of inactive precursors (proenzymes) and activated in the gastrointestinal tract. The pepsin precursors are called pepsinogens and are activated by gastric acid. Gastrointestinal System | Digestion & Absorption Gastrointestinal System | Digestion & Absorption Human gastric mucosa contains a number of related pepsinogens, which can be divided into two immunohistochemically distinct groups, pepsinogen I and pepsinogen II. Pepsinogen I is found only in acid-secreting regions, whereas pepsinogen II is also found in the pyloric region. Maximal acid secretion correlates with pepsinogen I levels. Gastrointestinal System | Digestion & Absorption Pepsins hydrolyze the bonds between aromatic amino acids such as phenylalanine or tyrosine and a second amino acid, so the products of peptic digestion are polypeptides of very diverse sizes. Because pepsins have a pH optimum of 1.6–3.2, their action is terminated when the gastric contents are mixed with the alkaline pancreatic juice in the duodenum and jejunum. Gastrointestinal System | Digestion & Absorption The pH of the intestinal contents in the duodenal bulb is 3.0–4.0, but rapidly rises; in the rest of the duodenum it is about 6.5. In the small intestine, the polypeptides formed by digestion in the stomach are further digested by the powerful proteolytic enzymes of the pancreas and intestinal mucosa. Gastrointestinal System | Digestion & Absorption Trypsin, the chymotrypsins, and elastase act at interior peptide bonds in the peptide molecules and are called endopeptidases. The formation of the active endopeptidases from their inactive precursors occurs only when they have reached their site of action, secondary to the action of the brush border hydrolase, enterokinase. Gastrointestinal System | Digestion & Absorption Gastrointestinal System | Digestion & Absorption The powerful protein-splitting enzymes of the pancreatic juice are secreted as inactive proenzymes. Trypsinogen is converted to the active enzyme trypsin by enterokinase when the pancreatic juice enters the duodenum. Enterokinase contains 41% polysaccharide, and this high polysaccharide content apparently prevents it from being digested itself before it can exert its effect. Gastrointestinal System | Digestion & Absorption Gastrointestinal System | Digestion & Absorption Trypsin converts chymotrypsinogens into chymotrypsins and other proenzymes into active enzymes. Trypsin can also activate trypsinogen; therefore, once some trypsin is formed, there is an auto-catalytic chain reaction. Enterokinase deficiency occurs as a congenital abnormality and leads to protein malnutrition. Gastrointestinal System | Digestion & Absorption Gastrointestinal System | Digestion & Absorption The carboxypeptidases of the pancreas are exopeptidases that hydrolyze the amino acids at the carboxyl ends of the polypeptides. Some free amino acids are liberated in the intestinal lumen, but others are liberated at the cell surface by the aminopeptidases, carboxypeptidases, endopeptidases, and dipeptidases in the brush border of the mucosal cells. Gastrointestinal System | Digestion & Absorption Gastrointestinal System | Digestion & Absorption Some dipeptides and tripeptides are actively transported into the intestinal cells and hydrolyzed by intracellular peptidases, with the amino acids entering the bloodstream. Thus, the final digestion to amino acids occurs in three locations: the intestinal lumen, the brush border, and the cytoplasm of the mucosal cells. Gastrointestinal System | Digestion & Absorption Proteins | Absorption Gastrointestinal System | Digestion & Absorption Absorption of Proteins : At least seven different transport systems transport amino acids into enterocytes. Five of these require Na+ and cotransport amino acids and Na+ in a fashion similar to the cotransport of Na+ and glucose. Two of these five also require Cl–. In two systems, transport is independent of Na+. Gastrointestinal System | Digestion & Absorption The dipeptides and tripeptides are transported into enterocytes by a system known as PepT1 (or peptide transporter 1) that requires H+ instead of Na+. Gastrointestinal System | Digestion & Absorption There is a very little absorption of larger peptides. In the enterocytes, amino acids released from the peptides by intracellular hydrolysis plus the amino acids absorbed from the intestinal lumen and brush border are transported out of the enterocytes along their basolateral borders by at least five transport systems. From there, they enter the hepatic portal blood. Absorption of amino acids is rapid in the duodenum and jejunum. Gastrointestinal System | Digestion & Absorption There is a little absorption in the ileum in health, because the majority of the free amino acids have already been assimilated at that point. Approximately 50% of the digested protein comes from ingested food, 25% from proteins in digestive juices, and 25% from desquamated mucosal cells. Only 2–5% of the protein in the small intestine escapes digestion and absorption. Gastrointestinal System | Digestion & Absorption Some of this is eventually digested by bacterial action in the colon. Almost all of the protein in the stools is not of dietary origin but comes from bacteria and cellular debris. Evidence suggests that the peptidase activities of the brush border and the mucosal cell cytoplasm are increased by resection of part of the ileum and that they are independently altered in starvation. Thus, these enzymes appear to be subject to homeostatic regulation. Gastrointestinal System | Digestion & Absorption In infants, moderate amounts of undigested proteins are also absorbed. The protein antibodies in maternal colostrum are largely secretory immunoglobulins (IgAs), the production of which is increased in the breast in late pregnancy. They cross the mammary epithelium by transcytosis and enter the circulation of the infant from the intestine, providing passive immunity against infections. Absorption is by endocytosis and subsequent exocytosis. Gastrointestinal System | Digestion & Absorption Absorption of intact proteins declines sharply after weaning, but adults still absorb small quantities. Foreign proteins that enter the circulation provoke the formation of antibodies, and the antigen–antibody reaction occurring on subsequent entry of more of the same protein may cause allergic symptoms. Thus, absorption of proteins from the intestine may explain the occurrence of allergic symptoms after eating certain foods. Gastrointestinal System | Digestion & Absorption The incidence of food allergy in children is said to be as high as 8%. Certain foods are more allergenic than others. Crustaceans, mollusks, and fish are common offenders, and allergic responses to legumes, cows’ milk, and egg white are also relatively frequent. However, in most individual food allergies do not occur, and there is an evidence for a genetic component in susceptibility. Gastrointestinal System | Digestion & Absorption Absorption of protein antigens, particularly bacterial and viral proteins, takes place in large microfold cells or M cells, specialized intestinal epithelial cells that overlie aggregates of lymphoid tissue (Peyer patches). These cells pass the antigens to the lymphoid cells, and lymphocytes are activated. The activated lymphoblasts enter the circulation, but they later return to the intestinal mucosa and other epithelia, where they secrete IgA in response to subsequent exposures to the same antigen. Gastrointestinal System | Digestion & Absorption Nucleic Acids Gastrointestinal System | Digestion & Absorption Nucleic Acids: Nucleic acids are split into nucleotides in the intestine by the pancreatic nucleases, and the nucleotides are split into the nucleosides and phosphoric acid by enzymes that appear to be located on the luminal surfaces of the mucosal cells. The nucleosides are then split into their constituent sugars and purine and pyrimidine bases. Gastrointestinal System | Digestion & Absorption The bases are absorbed by active transport. Families of equilibrative (ie, passive) and concentrative (ie, secondary active) nucleoside transporters have recently been identified and are expressed on the apical membrane of enterocytes. Lipids | Digestion Gastrointestinal System | Digestion & Absorption Fat Digestion: A lingual lipase is secreted by Ebner glands on the dorsal surface of the tongue in some species, and the stomach also secretes a lipase. They are of little quantitative significance for lipid digestion other than in the setting of pancreatic insufficiency, but they may generate free fatty acids that signal to most distal parts of the gastrointestinal tract (eg, causing the release of CCK). Gastrointestinal System | Digestion & Absorption Most fat digestion therefore begins in the duodenum, pancreatic lipase being one of the most important enzymes involved. This enzyme hydrolyzes the 1- and 3-bonds of the triglycerides (triacylglycerols) with relative ease but acts on the 2-bonds at a very low rate, so the principal products of its action are free fatty acids and 2- monoglycerides (2-monoacylglycerols). Gastrointestinal System | Digestion & Absorption Gastrointestinal System | Digestion & Absorption It acts on fats that have been emulsified (see below). Its activity is facilitated when an amphipathic helix that covers the active site like a lid is bent back. Colipase, a protein with a molecular weight of about 11,000, is also secreted in the pancreatic juice, and when this molecule binds to the –COOHterminal domain of the pancreatic lipase, opening of the lid is facilitated. Gastrointestinal System | Digestion & Absorption Colipase is secreted in an inactive preform and is activated in the intestinal lumen by trypsin. Colipase is also critical for the action of lipase because it allows lipase to remain associated with droplets of dietary lipid even in the presence of bile acids. Gastrointestinal System | Digestion & Absorption Another pancreatic lipase that is activated by bile acids has been characterized. This 100,000-kDa cholesterol esteras represents about 4% of the total protein in pancreatic juice. In adults, pancreatic lipase is 10–60 times more active, but unlike pancreatic lipase, cholesterol esterase catalyzes the hydrolysis of cholesterol esters, esters of fat-soluble vitamins, and phospholipids, as well as triglycerides. A very similar enzyme is found in human milk. Gastrointestinal System | Digestion & Absorption Fats are relatively insoluble, which limits their ability to cross the unstirred layer and reach the surface of the mucosal cells. However, they are finely emulsified in the small intestine by the detergent action of bile acids, phosphatidylcholine, and monoglycerides. When the concentration of bile acids in the intestine is high, as it is after contraction of the gallbladder, lipids and bile acids interact spontaneously to form micelles. Gastrointestinal System | Digestion & Absorption Gastrointestinal System | Digestion & Absorption These cylindrical aggregates take up lipids, and although their lipid concentration varies, they generally contain fatty acids, monoglycerides, and cholesterol in their hydrophobic centers. Micellar formation further solubilizes the lipids and provides a mechanism for their transport to the enterocytes. Thus, the micelles move down their concentration gradient through the unstirred layer to the brush border of the mucosal cells. Gastrointestinal System | Digestion & Absorption The lipids diffuse out of the micelles, and a saturated aqueous solution of the lipids is maintained in contact with the brush border of the mucosal cells. Lipids collect in the micelles, with cholesterol in the hydrophobic center and amphipathic phospholipids and monoglycerides lined up with their hydrophilic heads on the outside and their hydrophobic tails in the center. The micelles play an important role in keeping lipids in solution and transporting them to the brush border of the intestinal epithelial cells, where they are absorbed. Lipids | Absorption Gastrointestinal System | Digestion & Absorption Fat Absorption : Traditionally, lipids were thought to enter the enterocytes by passive diffusion, but some evidence now suggests that carriers are involved. Inside the cells, the lipids are rapidly esterified, maintaining a favorable concentration gradient from the lumen into the cells. There are also carriers that export certain lipids back into the lumen, thereby limiting their oral availability. This is the case for plant sterols as well as cholesterol. Gastrointestinal System | Digestion & Absorption The fate of the fatty acids in enterocytes depends on their size. Fatty acids containing less than 10–12 carbon atoms are water-soluble enough that they pass through the enterocyte unmodified and are actively transported into the portal blood. They circulate as free (unesterified) fatty acids. The fatty acids containing more than 10–12 carbon atoms are too insoluble for this. Gastrointestinal System | Digestion & Absorption Gastrointestinal System | Digestion & Absorption They are re-esterified to triglycerides in the enterocytes. In addition, some of the absorbed cholesterol is esterified. The triglycerides and cholesterol esters are then coated with a layer of protein, cholesterol, and phospholipid to form chylomicrons. These leave the cell and enter the lymphatics, because they are too large to pass through the junctions between capillary endothelial cells. Gastrointestinal System | Digestion & Absorption In mucosal cells, most of the triglyceride is formed by the acylation of the absorbed 2-monoglycerides, primarily in the smooth endoplasmic reticulum. However, some of the triglyceride is formed from glycerophosphate, which in turn is a product of glucose catabolism. Glycerophosphate is also converted into glycerophospholipids that participate in chylomicron formation. Gastrointestinal System | Digestion & Absorption The acylation of glycerophosphate and the formation of lipoproteins occur in the rough endoplasmic reticulum. Carbohydrate moieties are added to the proteins in the Golgi apparatus, and the finished chylomicrons are extruded by exocytosis from the basolateral aspect of the cell. Gastrointestinal System | Digestion & Absorption Absorption of long-chain fatty acids is greatest in the upper parts of the small intestine, but appreciable amounts are also absorbed in the ileum. On a moderate fat intake, 95% or more of the ingested fat is absorbed. The processes involved in fat absorption are not fully mature at birth, and infants fail to absorb 10–15% of ingested fat. Thus, they are more susceptible to the ill effects of disease processes that reduce fat absorption. Absorption of Iron Gastrointestinal System | Digestion & Absorption In adults, the amount of iron lost from the body is relatively small. The losses are generally unregulated, and total body stores of iron are regulated by changes in the rate at which it is absorbed from the intestine. Men lose about 0.6 mg/d, largely in the stools. Premenopausal women have a variable, larger loss averaging about twice this value because of the additional iron lost during menstruation. Gastrointestinal System | Digestion & Absorption The average daily iron intake in the United States and Europe is about 20 mg, but the amount absorbed is equal only to the losses. Thus, the amount of iron absorbed is normally about 3– 6% of the amount ingested. Gastrointestinal System | Digestion & Absorption Various dietary factors affect the availability of iron for absorption; for example, the phytic acid found in cereals reacts with iron to form insoluble compounds in the intestine, as do phosphates and oxalates. Most of the iron in the diet is in the ferric (Fe3+) form, whereas it is the ferrous (Fe2+) form that is absorbed. Fe3+ reductase activity is associated with the iron transporter in the brush borders of the enterocytes. Gastrointestinal System | Digestion & Absorption Gastrointestinal System | Digestion & Absorption Gastric secretions dissolve the iron and permit it to form soluble complexes with ascorbic acid and other substances that aid its reduction to the Fe2+ form. The importance of this function in humans is indicated by the fact that iron deficiency anemia is a troublesome and relatively frequent complication of partial gastrectomy. Almost all iron absorption occurs in the duodenum. Gastrointestinal System | Digestion & Absorption Transport of Fe2+ into the enterocytes occurs via divalent metal transporter 1 (DMT1). Some is stored in ferritin, and the remainder is transported out of the enterocytes by a basolateral transporter named ferroportin 1. A protein called hephaestin (Hp) is associated with ferroportin 1. It is not a transporter itself, but it facilitates basolateral transport. Gastrointestinal System | Digestion & Absorption Gastrointestinal System | Digestion & Absorption In the plasma, Fe2+ is converted to Fe3+ and bound to the iron transport protein transferrin. This protein has two iron-binding sites. Normally, transferrin is about 35% saturated with iron, and the normal plasma iron level is about 130 μg/dL (23 μmol/L) in men and 110 μg/dL (19 μmol/L) in women. Heme binds to an apical transport protein in enterocytes and is carried into the cytoplasm. Gastrointestinal System | Digestion & Absorption In the cytoplasm, HO-2, a subtype of heme oxygenase, removes Fe2+ from the porphyrin and adds it to the intracellular Fe2+ pool. Seventy percent of the iron in the body is in hemoglobin, 3% in myoglobin, and the rest in ferritin, which is present not only in enterocytes, but also in many other cells. Apoferritin is a globular protein made up of 24 subunits. Gastrointestinal System | Digestion & Absorption Ferritin is readily visible under the electron microscope and has been used as a tracer in studies of phagocytosis and related phenomena. Ferritin molecules in lysosomal membranes may aggregate in deposits that contain as much as 50% iron. These deposits are called hemosiderin. Gastrointestinal System | Digestion & Absorption Intestinal absorption of iron is regulated by three factors: recent dietary intake of iron, the state of the iron stores in the body, and the state of erythropoiesis in the bone marrow. The normal operation of the factors that maintain iron balance is essential for health (Clinical Box 26– 2). Gastrointestinal System | Digestion & Absorption Clinical Box 26–2: Disorders of Iron Uptake : Iron deficiency causes anemia. Conversely, iron overload causes hemosiderin to accumulate in the tissues, producing hemosiderosis. Large amounts of hemosiderin can damage tissues, such as is seen in the common genetic disorder of hemochromatosis. Gastrointestinal System | Digestion & Absorption This syndrome is characterized by pigmentation of the skin, pancreatic damage with diabetes (“bronze diabetes”), cirrhosis of the liver, a high incidence of hepatic carcinoma, and gonadal atrophy. Hemochromatosis may be hereditary or acquired. The most common cause of the hereditary forms is a mutated HFE gene that is common in the white population. Gastrointestinal System | Digestion & Absorption It is located on the short arm of chromosome 6 and is closely linked to the HLA-A locus. It is still unknown precisely how mutations in HFE cause hemochromatosis, but individuals who are homogenous for HFE mutations absorb excess amounts of iron because HFE normally inhibits expression of the duodenal transporters that participate in iron uptake. Gastrointestinal System | Digestion & Absorption Acquired hemochromatosis occurs when the ironregulating system is overwhelmed by excess iron loads due to chronic destruction of red blood cells, liver disease, or repeated transfusions in diseases such as intractable anemia. Therapeutic Highlights If hereditary hemochromatosis is diagnosed before excessive amounts of iron accumulate in the tissues, life expectancy can be prolonged substantially by repeated withdrawal of blood. Chapter Summary Gastrointestinal System | Digestion & Absorption Chapter Summary: A typical mixed meal consists of carbohydrates, proteins, and lipids (the latter largely in the form of triglycerides). Each must be digested to allow its uptake into the body. Specific transporters carry the products of digestion into the body. In the process of carbohydrate assimilation, the epithelium can only transport monomers, whereas for proteins, short peptides can be absorbed in addition to amino acids. Gastrointestinal System | Digestion & Absorption The protein assimilation machinery, which rests heavily on the proteases in pancreatic juice, is arranged such that these enzymes are not activated until they reach their substrates in the small intestinal lumen. This is accomplished by the restricted localization of an activating enzyme, enterokinase. Lipids face special challenges to assimilation given their hydrophobicity. Bile acids solubilize the products of lipolysis in micelles and accelerate their ability to diffuse to the epithelial surface. Gastrointestinal System | Digestion & Absorption The assimilation of triglycerides is enhanced by this mechanism, whereas that of cholesterol and fat-soluble vitamins absolutely requires it. The catabolism of nutrients provides energy to the body in a controlled fashion, via stepwise oxidations and other reactions. A balanced diet is important for health, and certain substances obtained from the diet are essential to life. The caloric value of dietary intake must be approximately equal to energy expenditure for homeostasis.