Clinical Chemistry Enzymes PDF
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Uploaded by HearteningGyrolite8118
Bulacan State University
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Summary
This document provides an overview of enzymes, clinical chemistry, and related clinical tests. The document covers a range of topics, including enzyme definitions, various enzyme classes, and their roles in clinical contexts, with specific information regarding hepatic and cardiac enzyme profiles. The document may be useful for students of biochemistry and/or medicine.
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CLINICAL CHEMISTRY ENZYMES ENZYME METHODS SUBSTRATES FACTS...
CLINICAL CHEMISTRY ENZYMES ENZYME METHODS SUBSTRATES FACTS HEPATIC ENZYME PROFILE Catalyzes reaction ALP Bodansky Β-glyceroPO4 Optimum pH: 10 Liver Kidney Shenowara Jones Β-glyceroPO4 Greatly elevated in Paget’s DEFINITION OF TERMS Bone King-Armstrong p-nitrophenylPO4 disease Apoenzyme: protein portion of enzyme without cofactor Placenta Bessy Lowry-Brock p-nitrophenylPO4 Avoid using EDTA-Citrate- Holoenzyme: complete active enzyme Intestine Oxalate WBC Active site: site where enzymatic reaction occurs ALT (SGPT) Reitman-Frankel (DNPH) Alanine α-keto Liver-specific Allosteric site: site other than the active site Liver Marked elevation with Isoenzyme: forms of enzyme that are different from each other but still catalyzes RBCs viral hepatitis same reaction De ritis ratio: CATEGORIES >1 = viral; LD2 P-ACP: unaffected by Cu++, inactivated by PROPERTY OF MEDTECH REVIEW NOTES18 | P a g e CLINICAL CHEMISTRY Tartrate PSA Most useful for tumor marker for prostate cancer RR: 0-4ng/mL ACUTE MYOCARDIAL INFARCTION MARKERS (Bishop, Rodriguez, Coderes) Marker Onset (hours) Peak (hours) Duration (hours) Myoglobin 1-3 5-12 18-30 Trop I 3-4 10-24 7days up to 10-14days Trop T 3-6 12-18 5-10 days CK-MB 4-6 12-24 48-72 AST 6-8 24 5 days LDH 12-24 48-72 10-14 days PROPERTY OF MEDTECH REVIEW NOTES19 | P a g e
