Enzyme and Tumor Markers PDF

LECTURE ENZYME REFERENCE RANGE Pyruvate to Lactate 80-280 U/L @ 37°C Lactate Dehydrogenase Lactate to Pyruvate 100-225 U/L @ 37°C G6PD (Glucose-6-Phosphate Dehydrogenase...

LECTURE ENZYME REFERENCE RANGE Pyruvate to Lactate 80-280 U/L @ 37°C Lactate Dehydrogenase Lactate to Pyruvate 100-225 U/L @ 37°C G6PD (Glucose-6-Phosphate Dehydrogenase 10-15 U/g Hgb Aspartate Aminotransferase (AST) 8-40 or 5-30 U/L Alanine Aminotransferase (ALT) less than 55 or 6-37 U/L MALE: 6-45 U/L @ 37°C Gamma Glutamyl Transferase (GGT) FEMALE: 5-30 U/L @ 37°C Alkaline Phosphatase (ALP) 20-120 U/L @ 37°C ADULT: 6,000-12,000 U/L Pseudo/Acetylcholinesterase (PChE & AChE) INFANTS: lower Lipase 0-1.0 U/L LD1 – Heart, RBC, Renal Cortex LD2 – Heart, RBC, Renal Cortex LD3 – Lung, Lymphocyte, Spleen, Pancreas LD4 – Liver LD5 – Skeletal Muscle LDx/c – Human Testis, Sperm G6PD – RBC, Adrenal Cortex, Spleen, Thymus, Lymph Nodes CK – Skeletal Muscle, Cardiac Muscle, Brain INCREASE PATTERN IN AMI ENZYME RISES PEAK RETURN TO NORMAL LDH 12-24 hrs 48-72 hrs 10 days after post-AMI CK 4-8 hrs 12-24 hrs 48-72 hrs Total CK 2-4 hrs 12-36 hrs 2-4 days AST 6-8 hrs 12-24 hrs 5 days BLOOD HALF LIFE AST & ALT – 17 and 47 hrs mAST – 87 hrs HIGHEST ELEVATION LDH: Megaloblastic Anemia, Hemolytic Anemia, Advanced Malignancies (Leukemia, Lymphoma), Toxic Hepatitis CK: Duchenne Muscular Dystrophy AST: Acute Liver Disease (Acute Viral Hepatitis) GGT: Intra/Extrahepatic Cholestasis BONE ALP: Paget’s Disease INCREASE PATTERN IN ACUTE PANCREATITIS ENZYME RISES PEAK RETURN TO NORMAL Lipase 6 hrs 24 hrs 8-14 days Amylase 2-12 hrs 24 hrs 3-5 days CUSI, BIANCA ASHLEY MED224  LABORATORY ENZYME REFERENCE RANFE Total CK 30-200 U/L Creatine Kinase CK-MB