Dengue IgG/IgM Test PDF

**Dengue IgG/IgM test** **Principle:** Dengue rapid test kits are designed to quickly detect the presence of dengue virus or antibodies against it in human serum, plasma, or whole blood. The two primary components tested are the **NS1 antigen** and the **IgM/IgG antibodies**. The NS1 protein is a...

**Dengue IgG/IgM test** **Principle:** Dengue rapid test kits are designed to quickly detect the presence of dengue virus or antibodies against it in human serum, plasma, or whole blood. The two primary components tested are the **NS1 antigen** and the **IgM/IgG antibodies**. The NS1 protein is a non-structural protein produced by the dengue virus during the early stages of infection. The test utilizes a specific antibody that binds to the NS1 protein. When a serum sample is added, if NS1 is present, it will bind to the coated antibodies on the test strip. IgM and IgG antibodies are produced by the immune system in response to the dengue virus. The test contains immobilized antigens that specifically bind to IgM or IgG antibodies. When serum is added, any IgM or IgG antibodies will bind to the antigens on the test strip. A secondary antibody (usually conjugated to a dye or enzyme) is then introduced, which binds to the antibody-antigen complex, resulting in a color change. The presence of IgM indicates a recent infection, while IgG suggests a past infection. The appearance of control lines alongside test lines indicates whether the test is valid. The presence of test lines corresponding to NS1, IgM, or IgG indicates positive results, providing insight into the phase of the infection. **Materials required:** - Serum sample - Dengue NS1 antigen and IgM/IgG antibody test kit - Pipettes and tips - Microcentrifuge tubes - Test tubes - Syringe - Tourniquet - Alcohol swabs **Procedure:** 1. Collected blood samples from a classmate. 2. Prepared serum sample from the blood. 3. For NS1 antigen test, took the test strip and added specified volume of serum to the sample well of the test device. 4. Waited for the designated time (usually 20 minutes) for results to develop. 5. Made the observation. 6. For IgM/IgG antibody test, took the test strip and added the appropriate volume of serum to the designated wells of the test kit. 7. Incubated for the specified time (20 minutes) and read the results. **Observations:** Recorded the absence of test lines for both NS1 and IgM/IgG tests for the sample. **Conclusion:** Serological testing for dengue virus is a valuable tool for diagnosing dengue infections. Timely and accurate detection can aid in the management and treatment of affected individuals, ultimately reducing morbidity and mortality associated with the disease.