Bio621 Integrative Physiology Chapter 6 PDF

BIO621 Integrative Physiology CHAPTER 6 PROTEOMICS AND METABOLOMICS Nur Hilwani Ismail  FLOW OF DISCUSSION  What Is proteomics and metabolomics https://www.youtube.com/watch?v=BiyhyLh-zCQ...

BIO621 Integrative Physiology CHAPTER 6 PROTEOMICS AND METABOLOMICS Nur Hilwani Ismail  FLOW OF DISCUSSION  What Is proteomics and metabolomics https://www.youtube.com/watch?v=BiyhyLh-zCQ  Methods of measurement using NMR and FTIR spectroscopy NHI/BIO621/CHAPTER 6 2 NHI/BIO621/CHAPTER 6 3  Source: Beyene, B., & Haile, G. (2016). Review on proteomics technologies and its application for crop improvement. Innovative Systems Design and Engineering. NHI/BIO621/CHAPTER 6 4  PROTEOMICS  Proteomics is study of protein  It enable the identification of number protein in organism (which might differ from cell to cell over time)  Focus on change by mRNA( phosphorylation and glycosylation)  Variable due to cell development, cellular differentiation, cell cycle or carcinogenesis (Biomarker) NHI/BIO621/CHAPTER 6 5  APPLICATIONS OF PROTEOMICS  Bioinformatics  Agriculture  Protein interaction  Biomarker  Protein structure  Characterized brain cells at molecular level  Neurotoxicology  https://www.youtube.com/watch?v=ijBapoWdd6M NHI/BIO621/CHAPTER 6 6 NHI/BIO621/CHAPTER 6 7  METABOLOMICS  Study of substrates and product of metabolism that influenced both genetic and environmental factors  Molecules involved in the process : sugars, lipids, amino acids, fatty acids, phenolic compounds and alkaloids –the interaction rapidly change overtime  Molecules continuously absorbed, synthesised, degraded and interact with other molecules NHI/BIO621/CHAPTER 6 8 NHI/BIO621/CHAPTER 6 9  APPLICATION OF METABOLOMICS  Pharmaceutical  Preventive healthcare  Agricultural industries-improve genetic of plant  Biomarker (possible customized treatment)  https://www.youtube.com/watch?v=eCNy NHI/BIO621/CHAPTER 6 j5PzJQU 10  METHODS OF MEASUREMENT  NMR (Nuclear Magnetic Resonance) Spectroscopy https://www.youtube.com/watch?v=Sn3dNMv-67k  FTIR (Fourier Transform Infrared) Spectroscopy https://www.youtube.com/watch?v=KRoWMB3AR3s NHI/BIO621/CHAPTER 6 11  NMR  Observe local magnetic field around atomic nuclei  NMR signal is produced by excitation of the nuclei sample with radio waves into nuclear magnetic resonance later detected with radio receiver  https://www.youtube.com/watch?v=YFftG1B- mYA  https://www.youtube.com/watch?v=Sn3dNMv- 67k NHI/BIO621/CHAPTER 6 12  FTIR  Infrared radiation passed through a sample, some radiation is absorbed by the sample and some passes through (or transmitted)  Produce different fingerprint of chemical structure  https://www.youtube.com/watch?v=KRoWMB3A R3s NHI/BIO621/CHAPTER 6 13 NHI/BIO621/CHAPTER 6 14 NHI/BIO621/CHAPTER 6 15 NHI/BIO621/CHAPTER 6 16 NHI/BIO621/CHAPTER 6 17 NHI/BIO621/CHAPTER 6 18  Proteomics and drug discovery NHI/BIO621/CHAPTER 6 19 NHI/BIO621/CHAPTER 6 20 NHI/BIO621/CHAPTER 6 21  END OF THE SYLLABUS FOR BIO621 NHI/BIO621/CHAPTER 6 22