Chapter 26 Study Guide - Exploring Microbes in Ecosystems PDF
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This study guide details microbial ecology, including culturing techniques, molecular characterization, and in-situ observations. It covers learning outcomes related to microbial resistance to growth in labs and introduces enrichment cultures and methods for taxonomic classification. The guide also explores various identification techniques and their application in public health.
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**Chapter 26 Study Guide - Exploring Microbes in Ecosystems** **CHAPTER OVERVIEW** This chapter describes the science of microbial ecology. The importance of culturing to microbiology is discussed along with various culturing techniques to induce growth of difficult-to-culture microbes. The use of...
**Chapter 26 Study Guide - Exploring Microbes in Ecosystems** **CHAPTER OVERVIEW** This chapter describes the science of microbial ecology. The importance of culturing to microbiology is discussed along with various culturing techniques to induce growth of difficult-to-culture microbes. The use of molecular characterization for biological identification and methods for the in situ observation of microorganisms and the communities they inhabit is discussed. LEARNING OUTCOMES ================= Describe three reasons why most microorganisms resist growing in culture in the laboratory. What is an enrichment culture? Describe at least two new approaches used to coax growth in the laboratory of previous uncultured microbes. Describe some commonly used methods used to determine taxonomic classification of a microorganism. Compare sequence and non-sequence based methods used for microbial identification. If two different bacteria have an orthologous gene based on sequence analysis, does mean that the gene product is will be functionally identical? Determine if the following techniques can identify a microbe at the genus, species, and strain level? technique ID genus? ID species? ID strain? ----------------------- ----------- ------------- ------------ DNA/DNA hybridization SSU rRNA Sequence G/C content MLST RFLP ANI Why is it important to use microbial taxonomic identification methods in public health professions? Provide an example of how microbial identification supports public health. What is the difference between *in vitro* and *in situ* studies? Explain why microbial communities are often investigated *in situ.* How has metagenomics changed how we assess microbial diversity? Describe how microelectrode measurements can be important tools in the study of microbial community activity.
