ANAPHY LAB NOTES - Microscopy PDF
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Uploaded by EnthralledThallium
New Era University
Josh Macapallag
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Summary
This document provides notes on microscope basics, including parts, figures, and functions. It covers the history of microscopy, including key figures like Zacharias Janssen and Antonie van Leeuwenhoek.
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Professor Josh Macapallag ANAPHY 1-CMT-1B 3. R evolving nosepiece - circular piece that L1: MICROSCOPE BASICS...
Professor Josh Macapallag ANAPHY 1-CMT-1B 3. R evolving nosepiece - circular piece that L1: MICROSCOPE BASICS allows rotation of different objective lenses 4. Arm - used for transporting the microscope 5. Stage - the flat surface below the objective. The microscope 6. Iris diaphragm - like an eye, it controlsthe ★ From the Greek workmicroscopium amount of light that passes through the ★ Mikros -small ;Skopein -look at bottom of the stage ★ Instrument of viewing 7. Coarse adjustment knob - used for quick ★ Allowed scientists to make newinsightson initial focusing. The bigger between the two the body and diseases 8. Fine adjustment knob - used to move the stage closer slowly for precise focus. The Notable figures: smaller between the two 1. Zacharias Janssen 9. Light source - provides light for the - created one of the earliest compound microscope microscopes (1600) 10.Base - a square-shaped support for 2. Antonie Van Leeuwenhoek transporting the microscope - made simple microscopes with his own 11.Objective lenses lenses. ★ Scanning (4x) - initial location - observed animal, blood, and sperm cells ★ Lowpower(10x)-initiallocation,or 3. Robert Hook for observing specimens that don’t - published Micrographia, copper-plate need high magnification illustrations. ★ High power (40x,43x,45x)-40xis - saw pores in a cork that resembled commonly used, while 43x and 45x honeycombs are considered high-dry powers - credited for naming the cell, the building (does not need oil) blocks of life ★ Oil immersion (100x) - used for extremely small specimens such as Main component:Compound light microscope bacteria and blood cells. It needs ★ Has compound lenses that magnifyobjects. cedar wood oil to function. It bends or refracts light Parts of the microscope 1. Eyepiece-alsoknownastheocularlens,is located at the top of the microscope and is responsible for magnifying an object 2. Body / eye / ocular tube - cylindricalpiece that connects the eyepiece to the objective lens. It holds the ocular lens in place
