Protein Separation Techniques & G6PD Demonstration 4 PDF

**MATRIX. NO. : A211760** **NAME : INTAN AQEELAH MUSFIRAH BINTI MUSA** **[DEMONSTRATION 4 : PROTEIN SEPARATION TECHNIQUES & G6PD]** **(TOTAL 100 marks = 100%)** This session is divided into **FIVE (5)** main activities: 1. **ACTIVITY 1**: The short video on selected protein purification te...

**MATRIX. NO. : A211760** **NAME : INTAN AQEELAH MUSFIRAH BINTI MUSA** **[DEMONSTRATION 4 : PROTEIN SEPARATION TECHNIQUES & G6PD]** **(TOTAL 100 marks = 100%)** This session is divided into **FIVE (5)** main activities: 1. **ACTIVITY 1**: The short video on selected protein purification techniques AND to answer the questions. (Please visit Youtube: AN INTRODUCTION TO BASIC PROTEIN PURIFICATION (part 1 of 2) at [***https://youtu.be/dqFeW9nPJBc***](https://youtu.be/dqFeW9nPJBc)) (8:55 mins). 2. **ACTIVITY 2**: The short video on Understanding Crystallography and to answer the questions. (Please visit Youtube: Part 1: From Proteins to Crystals at ***[https://youtu.be/gLsC4wlrR2A]***) (7:47 mins) 3. **ACTIVITY 3**: The short video on Understanding Crystallography and to answer the questions. (Please visit Youtube: Part 2: From Crystals to Diamond at ***[https://youtu.be/WJKvDUo3KRk]***) (8:14 mins) 4. **ACTIVITY 4**: The short video on G6PD and to answer the questions. (Please visit Youtube: MEDICAL SCHOOL - GLUCOSE 6 PHOSPHATE DEHYDROGENASE (G6PD) DEFICIENCY at [***https://youtu.be/opZwQCYg9ao***](https://youtu.be/opZwQCYg9ao)) (9:27 mins). 5. **ACTIVITY 5**: The short video on G6PD and to answer the questions. (Please visit Youtube: G6PD Diet: Foods to Eat & Avoid at [***https://youtu.be/PZ3IOQYnTGM?si=UV6nFDyz7IqG1Q0M***](https://youtu.be/PZ3IOQYnTGM?si=UV6nFDyz7IqG1Q0M)) (5:51 mins) **[\ ]** **ACTIVITY 1:** AN INTRODUCTION TO BASIC PROTEIN PURIFICATION [ ] **(20 marks)** **Question 1**: Why protein isolation techniques are important in analysis of proteins? Give **TWO (2)** reasons. *Answer:* (4 marks) **Question 2**: List GENERAL steps in protein purification. *Answer:* (4 marks) **Question 3**: Briefly explain on various sources of sample for protein purification. *Answer:* (5 marks) **Question 4**: Briefly explain on disruption method of samples/specimen in protein purification. *Answer:* (5 marks) **Question 5**: List **TWO (2)** techniques for separating bulk mixtures in protein purification. *Answer:* (2 marks) **[\ ]** **ACTIVITY 2: UNDERSTANDING CRYSTALLOGRAPHY -- PART 1** **FROM PROTEINS TO CRYSTALS (20 marks)** **Question 1**: How can you determine the structure of a complex molecule from a single crystal via X-rays crystallography technique? *Answer:* (5 marks) **Question 2**: Briefly explain about the preparation for producing protein crystals. *Answer:* (5 marks) **Question 3**: Briefly explain about the key processes involved in the crystallisation laboratory. *Answer:* (6 marks) **Question 4**: Give FOUR (4) examples of the molecules used in this video for 3D structures generation via X-rays crystallography technique. *Answer:* (4 marks) **[\ ]** **ACTIVITY 3: UNDERSTANDING CRYSTALLOGRAPHY -- PART 2** **FROM CRYSTALS TO DIAMOND (20 marks)** **Question 1**: Briefly explain about the use of X-rays radiation in predicting the structure of molecules. *Answer:* (4 marks) **Question 2**: What were the limitations in earlier technique invented by the crystallographer scientist, William Henry Bragg (1920s) for X-rays generator? *Answer:* (3 marks) **Question 3**: What are the advancements made nowadays by using synchrotrons for X-rays generator. *Answer:* (3 marks) **Question 4**: Briefly explain the whole event starts from the core of synchrotrons until producing X-rays radiation and later to perform diffraction patterns analysis. *Answer:* (6 marks) **Question 5**: Briefly explain the association between diffraction patterns analysed with the predicted 3D structure of molecules. *Answer:* (4 marks) **[\ ]** **ACTIVITY 4: GLUCOSE 6 PHOSPHATE DEHYDROGENASE (G6PD) DEFICIENCY (20 marks)** **Question 1**: Briefly explain about the biological function of glucose-6-phosphate dehydrogenase (G6PD). *Answer:* (4 marks) **Question 2**: What is selective advantage of G6PD deficiency? *Answer:* (4 marks) **Question 3**: Briefly explain about severity level of G6PD. *Answer:* (4 marks) **Question 4**: Briefly explain on G6PD deficiency presentation in neonatal jaundice. *Answer:* (4 marks) **Question 5**: Briefly explain about fluorescent spot test in blood test. *Answer:* (4 marks) **ACTIVITY 5: G6PD DIET - FOODS TO EAT & AVOID (20 marks)** **Question 1**: Briefly explain about the typical clinical condition called haemolytic anaemia observed in patient with G6PD deficiency. *Answer:* (4 marks) **Question 2**: Name the foods that are safe to be consumed by patient with G6PD deficiency. *Answer:* (4 marks) **Question 3**: How you relate oxidative stress in erythrocytes with G6PD function? *Answer:* (4 marks) **Question 4**: Briefly explain in diet management programmes, why certain foods are recommended to be avoided by patient with G6PD deficiency. *Answer:* (4 marks) **Question 5**: Give FOUR (4) your recommendations to patients with G6PD deficiency for their healthy lifestyles. *Answer:* (4 marks) - *GOOD LUCK AND HAPPY LEARNING* -