PHBT402 Pharmaceutical Microbiology Assignment PDF

PHBTt402 Introduction to Pharmaceutical Microbiology Assign. # 1 Assignment on Lecture # 1 I. Multiple Choice Questions (Choose the one alternative that best completes the statement or a...

PHBTt402 Introduction to Pharmaceutical Microbiology Assign. # 1 Assignment on Lecture # 1 I. Multiple Choice Questions (Choose the one alternative that best completes the statement or answers the question) d. Fungi 4. Cell wall made of cellulose; 1. Which of the following distinguish the field of photosynthetic microbiology from other fields of biology? e. Helminths 5. Unicellular, complex cell structure A. the size of the organism studied lacking a cell wall f. Protozoa 6. Multicellular animals B. the techniques used to study organisms g. Viruses 7. Prokaryote without peptidoglycan regardless of their size cell wall C. both the size of the organism studied and the techniques employed in the study of organisms 6. Acid-fast organisms such as Mycobacterium tuberculosis resist decolorization by an acid-alcohol wash because of D. neither the size of the organism studied nor the the high concentration of __________ in their cell walls. techniques employed in the study of organisms A. proteins regardless of their size B. carbohydrates C. lipids 2. Which of the following is a beneficial activity of D. peptidoglycan microorganisms? A. Some microorganisms are used as food for humans. 7. Which of the following is not a characteristic of bacteria? B. Some microorganisms use carbon dioxide. A. are prokaryotic C. Some microorganisms provide nitrogen for plant B. have peptidoglycan cell walls growth. C. have the same shape D. Some microorganisms are used in sewage treatment D. grow by binary fission processes. E. have the ability to move E. all of the above 8. In the Gram-staining procedure, the primary stain is 3. It has been said that bacteria are essential for the A. iodine. existence of life on Earth. Which of the following is the B. safranin. essential function performed by bacteria? C. crystal violet. A. control insect populations D. alcohol. B. directly provide food for humans C. decompose organic material and recycle elements 9. In the Gram-staining procedure, the decolorizer is D. cause disease A. iodine. E. produce human hormones such as insulin B. safranin. C. crystal violet. 4. Into which field of microbiology would the following D. alcohol. scientists best fit? Researcher Who Field 10. In the Gram-staining procedure, the counterstain is a. Studies biodegradation of 1. Biotechnology A. iodine. toxic wastes B. safranin. b. Studies the causative agent 2. Immunology C. crystal violet. of Ebola virus disease D. alcohol. c. Studies the production of 3. Microbial ecology human proteins by bacteria 11. In the Gram-staining procedure, the mordant is d. Studies the symptoms of 4. Microbial genetics A. iodine. AIDS B. safranin. e. Studies the production of 5. Microbial physiology C. crystal violet. toxin by E. coli D. alcohol. f. Studies biodegradation of 6. Molecular biology pollutants 12. After the primary stain has been added but before the g. Develops gene therapy for a 7. Mycology decolorizer has been used, gram-positive organisms are disease stained __________ and gram-negative organisms are h. Studies the fungus Candida 8. Virology stained __________. albicans A. purple; purple B. purple; colorless 5. Match the microorganisms in column A to their C. purple; pink descriptions in column B. D. pink; pink Column A Column B a. Archaea 1. Not composed of cells b. Algae 2. Cell wall made of chitin c. Bacteria 3. Cell wall made of peptidoglycan  PHBTt402 Introduction to Pharmaceutical Microbiology Assign. # 1 13. After the decolorizer has been added, gram-positive C. Why does penicillin have no effect on most gram- organisms are stained __________ and gram-negative negative cells? organisms are stained __________. A. purple; purple D. How do essential molecules enter cells through B. purple; colorless each wall? C. purple; pink D. pink; pink E. Which cell wall is toxic to humans? 14. After the secondary stain has been added, gram-positive 19. Which of the following is not a distinguishing characteristic organisms are stained __________ and gram-negative of prokaryotic cells? organisms are stained __________. A. They usually have a single, circular chromosome. A. purple; purple B. They have 70S ribosomes. B. purple; colorless C. They have cell walls containing peptidoglycan. C. purple; pink D. Their DNA is not associated with histones. D. pink; pink E. They lack a plasma membrane. 15. If the decolorizer is left on too long in the Gram-staining 20. Use the following choices to answer questions 1–3. procedure, gram-positive organisms will be stained A. No change will result; the solution is isotonic. __________ and gram-negative organisms will be stained B. Water will move into the cell. __________. C. Water will move out of the cell. A. purple; blue D. The cell will undergo osmotic lysis. B. purple; colorless E. Sucrose will move into the cell from an area of C. purple; pink higher concentration to one of lower concentration. D. pink; pink 1. Which statement best describes what happens when a gram positive bacterium is placed in distilled water and 16. If the decolorizer is not left on long enough in the Gram- penicillin? staining procedure, gram-positive organisms will be 2. Which statement best describes what happens when a stained __________ and gram-negative organisms will be gram negative bacterium is placed in distilled water and stained __________. penicillin? A. purple; purple 3. Which statement best describes what happens when a B. purple; colorless gram positive bacterium is placed in an aqueous solution of C. purple; pink lysozyme and 10% sucrose? D. pink; pink 17. Which of the following is considered to be a differential staining procedure? A. Gram stain B. Acid-fast stain C. both Gram stain and Acid-fast stain D. Leifson’s flagella stain 18. Answer the following questions using the diagrams provided, which represent cross sections of bacterial cell walls. A. Which diagram represents a gram-positive bacterium? How can you tell? B. Explain how the Gram stain works to distinguish these two types of cell walls.